Artichoke extracts with potential application in chemoprevention and inflammatory processes
Abstract The aim of this work is to study three cultivars of artichoke (Cynara cardunculus var. scolymus): Gauchito, Guri and Oro Verde in terms of their in vitro chemoprevention and anti-inflammatory properties. These cultivars show good productive performance. The phenolic composition of their fre...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade de São Paulo
2022-05-01
|
| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100575&lng=en&tlng=en |
| _version_ | 1818551141068177408 |
|---|---|
| author | Rosana Rotondo Pablo Santa Cruz Marianela Masin Milagros Bürgi Javier Girardini Stella M. García Gustavo R. Rodríguez Ricardo L. E. Furlan Andrea M. Escalante |
| author_facet | Rosana Rotondo Pablo Santa Cruz Marianela Masin Milagros Bürgi Javier Girardini Stella M. García Gustavo R. Rodríguez Ricardo L. E. Furlan Andrea M. Escalante |
| author_sort | Rosana Rotondo |
| collection | DOAJ |
| description | Abstract The aim of this work is to study three cultivars of artichoke (Cynara cardunculus var. scolymus): Gauchito, Guri and Oro Verde in terms of their in vitro chemoprevention and anti-inflammatory properties. These cultivars show good productive performance. The phenolic composition of their fresh leaves and edible bracts was analyzed by high performance liquid chromatography and high resolution mass spectrometry (HPLC-HRMS), showing mainly caffeoylquinic acids and flavonoids. Caffeoylquinic acids were quantified and the highest content was found in Gauchito cultivar. In this cultivar, the content of dicaffeoylquinic acids in fresh bracts was six times higher than that in fresh leaves (10064.5 ± 378.3 mg/kg versus 1451.0 ± 209.3 mg/kg respectively). Luteolin flavonoids were detected in leaves. The extracts from fresh bracts and leaves were assessed in their in vitro bioactivity against human neuroblastoma cells (SH-SY5Y). Inhibition of SH-SY5Y cells proliferation by Gauchito and Guri leaf extracts (8 µg/mL) was higher than 50 %. The leaf extracts of the same cultivars showed an inhibitory effect on human interferon IFN-I, decreasing its activity 50% at 40 µg/mL. Interestingly, the bract extracts did not show in vitro bioactivity at these concentrations, nor did the pure compounds chlorogenic acid, cynarin, apigenin and luteolin (at 2 µg/mL). These results suggest that Gauchito and Guri leaf extracts have potential for human neuroblastoma chemoprevention and treatment of inflammatory processes. |
| first_indexed | 2024-12-12T08:55:55Z |
| format | Article |
| id | doaj.art-cb1272beeb044d85ad38eb54a6695b6e |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-12-12T08:55:55Z |
| publishDate | 2022-05-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-cb1272beeb044d85ad38eb54a6695b6e2022-12-22T00:29:59ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902022-05-015810.1590/s2175-97902022e19238Artichoke extracts with potential application in chemoprevention and inflammatory processesRosana RotondoPablo Santa CruzMarianela MasinMilagros BürgiJavier GirardiniStella M. GarcíaGustavo R. RodríguezRicardo L. E. FurlanAndrea M. Escalantehttps://orcid.org/0000-0001-8279-5174Abstract The aim of this work is to study three cultivars of artichoke (Cynara cardunculus var. scolymus): Gauchito, Guri and Oro Verde in terms of their in vitro chemoprevention and anti-inflammatory properties. These cultivars show good productive performance. The phenolic composition of their fresh leaves and edible bracts was analyzed by high performance liquid chromatography and high resolution mass spectrometry (HPLC-HRMS), showing mainly caffeoylquinic acids and flavonoids. Caffeoylquinic acids were quantified and the highest content was found in Gauchito cultivar. In this cultivar, the content of dicaffeoylquinic acids in fresh bracts was six times higher than that in fresh leaves (10064.5 ± 378.3 mg/kg versus 1451.0 ± 209.3 mg/kg respectively). Luteolin flavonoids were detected in leaves. The extracts from fresh bracts and leaves were assessed in their in vitro bioactivity against human neuroblastoma cells (SH-SY5Y). Inhibition of SH-SY5Y cells proliferation by Gauchito and Guri leaf extracts (8 µg/mL) was higher than 50 %. The leaf extracts of the same cultivars showed an inhibitory effect on human interferon IFN-I, decreasing its activity 50% at 40 µg/mL. Interestingly, the bract extracts did not show in vitro bioactivity at these concentrations, nor did the pure compounds chlorogenic acid, cynarin, apigenin and luteolin (at 2 µg/mL). These results suggest that Gauchito and Guri leaf extracts have potential for human neuroblastoma chemoprevention and treatment of inflammatory processes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100575&lng=en&tlng=enCynara cardunculus var. scolymus. Neuroblastoma. InterferonPhenolic compounds |
| spellingShingle | Rosana Rotondo Pablo Santa Cruz Marianela Masin Milagros Bürgi Javier Girardini Stella M. García Gustavo R. Rodríguez Ricardo L. E. Furlan Andrea M. Escalante Artichoke extracts with potential application in chemoprevention and inflammatory processes Brazilian Journal of Pharmaceutical Sciences Cynara cardunculus var. scolymus. Neuroblastoma. Interferon Phenolic compounds |
| title | Artichoke extracts with potential application in chemoprevention and inflammatory processes |
| title_full | Artichoke extracts with potential application in chemoprevention and inflammatory processes |
| title_fullStr | Artichoke extracts with potential application in chemoprevention and inflammatory processes |
| title_full_unstemmed | Artichoke extracts with potential application in chemoprevention and inflammatory processes |
| title_short | Artichoke extracts with potential application in chemoprevention and inflammatory processes |
| title_sort | artichoke extracts with potential application in chemoprevention and inflammatory processes |
| topic | Cynara cardunculus var. scolymus. Neuroblastoma. Interferon Phenolic compounds |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100575&lng=en&tlng=en |
| work_keys_str_mv | AT rosanarotondo artichokeextractswithpotentialapplicationinchemopreventionandinflammatoryprocesses AT pablosantacruz artichokeextractswithpotentialapplicationinchemopreventionandinflammatoryprocesses AT marianelamasin artichokeextractswithpotentialapplicationinchemopreventionandinflammatoryprocesses AT milagrosburgi artichokeextractswithpotentialapplicationinchemopreventionandinflammatoryprocesses AT javiergirardini artichokeextractswithpotentialapplicationinchemopreventionandinflammatoryprocesses AT stellamgarcia artichokeextractswithpotentialapplicationinchemopreventionandinflammatoryprocesses AT gustavorrodriguez artichokeextractswithpotentialapplicationinchemopreventionandinflammatoryprocesses AT ricardolefurlan artichokeextractswithpotentialapplicationinchemopreventionandinflammatoryprocesses AT andreamescalante artichokeextractswithpotentialapplicationinchemopreventionandinflammatoryprocesses |