Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis
The NSP4 protein is a multifunctional protein that plays a role in the morphogenesis and pathogenesis of the rotavirus. Although NSP4 is considered an enterotoxin, the relationship between gastroenteritis severity and amino acid variations in NSP4 of the human rotavirus remains unclear. In this stud...
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| Format: | Article |
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MDPI AG
2013-03-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/5/3/792 |
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| author | Juan F. Contreras Reyes S. Tamez Carlos E. Hernández Cristina Rodríguez Guadalupe González-Ochoa Griselda E. Menchaca |
| author_facet | Juan F. Contreras Reyes S. Tamez Carlos E. Hernández Cristina Rodríguez Guadalupe González-Ochoa Griselda E. Menchaca |
| author_sort | Juan F. Contreras |
| collection | DOAJ |
| description | The NSP4 protein is a multifunctional protein that plays a role in the morphogenesis and pathogenesis of the rotavirus. Although NSP4 is considered an enterotoxin, the relationship between gastroenteritis severity and amino acid variations in NSP4 of the human rotavirus remains unclear. In this study, we analyzed the sequence diversity of NSP4 and the severity of gastroenteritis of children with moderate to severe gastroenteritis. The rotavirus-infected children were hospitalized before the rotavirus vaccine program in Mexico. All children had diarrhea within 1-4 days, 44 (88%) were vomiting and 35 (70%) had fevers. The severity analysis showed that 13 (26%) cases had mild gastroenteritis, 23 (46%) moderate gastroenteritis and 14 (28%) severe. NSP4 phylogenetic analysis showed three clusters within the genotype E1. Sequence analysis revealed similar mutations inside each cluster, and an uncommon variation in residue 144 was found in five of the Mexican NSP4 sequences. Most of the amino acid variations were located in the VP4 and VP6 binding site domains, with no relationship to different grades of gastroenteritis. This finding indicates that severe gastroenteritis caused by the rotavirus appears to be related to diverse viral or cellular factors instead of NSP4 activity as a unique pathogenic factor. |
| first_indexed | 2024-12-23T06:37:43Z |
| format | Article |
| id | doaj.art-cb12e000046f44089e84f21ade74c032 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-23T06:37:43Z |
| publishDate | 2013-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-cb12e000046f44089e84f21ade74c0322022-12-21T17:56:45ZengMDPI AGViruses1999-49152013-03-015379280510.3390/v5030792Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe GastroenteritisJuan F. ContrerasReyes S. TamezCarlos E. HernándezCristina RodríguezGuadalupe González-OchoaGriselda E. MenchacaThe NSP4 protein is a multifunctional protein that plays a role in the morphogenesis and pathogenesis of the rotavirus. Although NSP4 is considered an enterotoxin, the relationship between gastroenteritis severity and amino acid variations in NSP4 of the human rotavirus remains unclear. In this study, we analyzed the sequence diversity of NSP4 and the severity of gastroenteritis of children with moderate to severe gastroenteritis. The rotavirus-infected children were hospitalized before the rotavirus vaccine program in Mexico. All children had diarrhea within 1-4 days, 44 (88%) were vomiting and 35 (70%) had fevers. The severity analysis showed that 13 (26%) cases had mild gastroenteritis, 23 (46%) moderate gastroenteritis and 14 (28%) severe. NSP4 phylogenetic analysis showed three clusters within the genotype E1. Sequence analysis revealed similar mutations inside each cluster, and an uncommon variation in residue 144 was found in five of the Mexican NSP4 sequences. Most of the amino acid variations were located in the VP4 and VP6 binding site domains, with no relationship to different grades of gastroenteritis. This finding indicates that severe gastroenteritis caused by the rotavirus appears to be related to diverse viral or cellular factors instead of NSP4 activity as a unique pathogenic factor.http://www.mdpi.com/1999-4915/5/3/792rotavirussevere gastroenteritisNSP4 |
| spellingShingle | Juan F. Contreras Reyes S. Tamez Carlos E. Hernández Cristina Rodríguez Guadalupe González-Ochoa Griselda E. Menchaca Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis Viruses rotavirus severe gastroenteritis NSP4 |
| title | Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis |
| title_full | Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis |
| title_fullStr | Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis |
| title_full_unstemmed | Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis |
| title_short | Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis |
| title_sort | mutation distribution in the nsp4 protein in rotaviruses isolated from mexican children with moderate to severe gastroenteritis |
| topic | rotavirus severe gastroenteritis NSP4 |
| url | http://www.mdpi.com/1999-4915/5/3/792 |
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