A study on karyotype of Assam Macaque, Macaca assamensis (Primate, Cercopithecidae) by using conventional staining method
This research was the first karyotypic study of Assam macaque (Macaca assamensis). Blood samples were taken from two males and two females kept in Nakhon Ratchasima Zoo and Songkhla Zoo. After the standard whole blood lymphocyte culture at 37ºC for 72 hr. in presence of Colchicine, the metaphase spr...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2005-11-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://www.sjst.psu.ac.th/journal/27-6-pdf/08-assam-macaque.pdf |
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| author | Bunjongrat, R. Khunsook, S. Kaensa, W. Tanomtong, A. |
| author_facet | Bunjongrat, R. Khunsook, S. Kaensa, W. Tanomtong, A. |
| author_sort | Bunjongrat, R. |
| collection | DOAJ |
| description | This research was the first karyotypic study of Assam macaque (Macaca assamensis). Blood samples were taken from two males and two females kept in Nakhon Ratchasima Zoo and Songkhla Zoo. After the standard whole blood lymphocyte culture at 37ºC for 72 hr. in presence of Colchicine, the metaphase spreads were performed on microscopic slides and air-dried. Conventional Giemsa's staining were applied to stain the chromosome. The results showed that the number of diploid chromosome of Assam macaque was 2n = 42, the fundamental number (NF) was 83 in male and 84 in female. The type of autosomes were 6 large metacentric, 8 large submetacentric, 4 medium metacentric, 12 medium submetacentric, 8 small metacentric and 2 small submetacentric chromosomes. In addition, a pair of the short arm of chromosome 13 showed clearly observable satellite chromosome. X-chromosome was the medium submetacentric and Y chromosome was the smallest telocentric chromosome.The karyotype formula for the male Assam macaque (Macaca assamensis) is as follows :2n (42) = L6 m+L8 sm+M4 m+M12 sm+S8 m+S2 sm+X+Y = L6 m+L8 sm+M m+M13 sm+S8 m+S2 sm+S1 t The karyotype formula for the female Assam macaque (Macaca assamensis) is as follows :2n (42) = L6 m+L8 sm+M4 m+M12 sm+S8 m+S2 sm +X+X = L6 m+L8 sm+M4 m+M14 sm+S8 m+S2 sm |
| first_indexed | 2024-12-19T22:03:54Z |
| format | Article |
| id | doaj.art-cb12ed784b7647dcb78cddee07dd073b |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-19T22:03:54Z |
| publishDate | 2005-11-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-cb12ed784b7647dcb78cddee07dd073b2022-12-21T20:04:04ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952005-11-0127611991208A study on karyotype of Assam Macaque, Macaca assamensis (Primate, Cercopithecidae) by using conventional staining methodBunjongrat, R.Khunsook, S.Kaensa, W.Tanomtong, A.This research was the first karyotypic study of Assam macaque (Macaca assamensis). Blood samples were taken from two males and two females kept in Nakhon Ratchasima Zoo and Songkhla Zoo. After the standard whole blood lymphocyte culture at 37ºC for 72 hr. in presence of Colchicine, the metaphase spreads were performed on microscopic slides and air-dried. Conventional Giemsa's staining were applied to stain the chromosome. The results showed that the number of diploid chromosome of Assam macaque was 2n = 42, the fundamental number (NF) was 83 in male and 84 in female. The type of autosomes were 6 large metacentric, 8 large submetacentric, 4 medium metacentric, 12 medium submetacentric, 8 small metacentric and 2 small submetacentric chromosomes. In addition, a pair of the short arm of chromosome 13 showed clearly observable satellite chromosome. X-chromosome was the medium submetacentric and Y chromosome was the smallest telocentric chromosome.The karyotype formula for the male Assam macaque (Macaca assamensis) is as follows :2n (42) = L6 m+L8 sm+M4 m+M12 sm+S8 m+S2 sm+X+Y = L6 m+L8 sm+M m+M13 sm+S8 m+S2 sm+S1 t The karyotype formula for the female Assam macaque (Macaca assamensis) is as follows :2n (42) = L6 m+L8 sm+M4 m+M12 sm+S8 m+S2 sm +X+X = L6 m+L8 sm+M4 m+M14 sm+S8 m+S2 smhttp://www.sjst.psu.ac.th/journal/27-6-pdf/08-assam-macaque.pdfcytogeneticschromosomeAssam macaque (Macaca assamensis) |
| spellingShingle | Bunjongrat, R. Khunsook, S. Kaensa, W. Tanomtong, A. A study on karyotype of Assam Macaque, Macaca assamensis (Primate, Cercopithecidae) by using conventional staining method Songklanakarin Journal of Science and Technology (SJST) cytogenetics chromosome Assam macaque (Macaca assamensis) |
| title | A study on karyotype of Assam Macaque, Macaca assamensis (Primate, Cercopithecidae) by using conventional staining method |
| title_full | A study on karyotype of Assam Macaque, Macaca assamensis (Primate, Cercopithecidae) by using conventional staining method |
| title_fullStr | A study on karyotype of Assam Macaque, Macaca assamensis (Primate, Cercopithecidae) by using conventional staining method |
| title_full_unstemmed | A study on karyotype of Assam Macaque, Macaca assamensis (Primate, Cercopithecidae) by using conventional staining method |
| title_short | A study on karyotype of Assam Macaque, Macaca assamensis (Primate, Cercopithecidae) by using conventional staining method |
| title_sort | study on karyotype of assam macaque macaca assamensis primate cercopithecidae by using conventional staining method |
| topic | cytogenetics chromosome Assam macaque (Macaca assamensis) |
| url | http://www.sjst.psu.ac.th/journal/27-6-pdf/08-assam-macaque.pdf |
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