Pathogenesis of Isolated Newcastle Disease Virus Genotype VII .1.1 in Turkey Poults in Egypt
Recurrent infection with Newcastle disease virus in flocks that have received vaccinations and high economic losses in Egypt in the last few years urged us to study the diversity and genetic changes in isolated NDV from chickens and its pathogenesis in other species such as turkey poults. Fifteen p...
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| Format: | Article |
| Language: | English |
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Assiut University
2022-09-01
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| Series: | Journal of Advanced Veterinary Research |
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| Online Access: | https://advetresearch.com/index.php/AVR/article/view/1046 |
| _version_ | 1797657007540404224 |
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| author | Ahmed M. E. Hegazy Amr Abd El-Fattah Bedair Hala M. N. Tolba |
| author_facet | Ahmed M. E. Hegazy Amr Abd El-Fattah Bedair Hala M. N. Tolba |
| author_sort | Ahmed M. E. Hegazy |
| collection | DOAJ |
| description |
Recurrent infection with Newcastle disease virus in flocks that have received vaccinations and high economic losses in Egypt in the last few years urged us to study the diversity and genetic changes in isolated NDV from chickens and its pathogenesis in other species such as turkey poults. Fifteen positive NDVs were isolated from chicken flocks suffering from a respiratory infection. Sequencing of three isolates out from the 15 NDV positive isolates (20%) revealed that NDV was genotype VII.1.1. When compared to other previously isolated worldwide and Egyptian strains, the three isolates’ amino acid sequences show (99.1-99.8 %) identity with genotype VII.1.1.Thirty four weeks old Black Burzi turkey poults, separated into two groups: control (n=15) and infected (n=15), were used to study the pathogenesis of the isolated NDV genotype VII.1.1. Each afflicted bird was given an inoculation with 0.1 mL of 106EID50 of NDV genotype VII.1.1( ND /chicken /Egypt /Dakahlia /31 /2020) at 4 weeks of age via an ocular route. Proventriculus, conjunctiva, lung, spleen, trachea, and caecal tonsil samples were collected from both groups at (6, 12, 24, 48 hours, and 5 days after infection) and tested for the presence of NDV using Quantitative Reverse transcription-polymerase Chain Reaction (QRT-PCR). The virus was found in the challenged birds’ spleen as soon as 12 hours after infection, followed by the lungs and trachea. After 2 and 5 days after NDV infection, histologically significant lesions were found, particularly in lymphoid organs. It is concluded that the presence of NDV in Egyptian flocks of chickens could induce major disease in commercial turkeys, necessitating the development of novel vaccinations based on the circulating NDV genotype VII.1.1 in Egypt to protect domestic poultry from recurrent infection.
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| first_indexed | 2024-03-11T17:39:08Z |
| format | Article |
| id | doaj.art-cb14dbbadd5849b0b06d50ecf2accc08 |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-03-11T17:39:08Z |
| publishDate | 2022-09-01 |
| publisher | Assiut University |
| record_format | Article |
| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-cb14dbbadd5849b0b06d50ecf2accc082023-10-18T14:26:00ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772022-09-01124Pathogenesis of Isolated Newcastle Disease Virus Genotype VII .1.1 in Turkey Poults in EgyptAhmed M. E. Hegazy0Amr Abd El-Fattah Bedair1Hala M. N. Tolba2Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt.Veterinary sector in Damietta, Veterinary organization, Egypt.Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt. Recurrent infection with Newcastle disease virus in flocks that have received vaccinations and high economic losses in Egypt in the last few years urged us to study the diversity and genetic changes in isolated NDV from chickens and its pathogenesis in other species such as turkey poults. Fifteen positive NDVs were isolated from chicken flocks suffering from a respiratory infection. Sequencing of three isolates out from the 15 NDV positive isolates (20%) revealed that NDV was genotype VII.1.1. When compared to other previously isolated worldwide and Egyptian strains, the three isolates’ amino acid sequences show (99.1-99.8 %) identity with genotype VII.1.1.Thirty four weeks old Black Burzi turkey poults, separated into two groups: control (n=15) and infected (n=15), were used to study the pathogenesis of the isolated NDV genotype VII.1.1. Each afflicted bird was given an inoculation with 0.1 mL of 106EID50 of NDV genotype VII.1.1( ND /chicken /Egypt /Dakahlia /31 /2020) at 4 weeks of age via an ocular route. Proventriculus, conjunctiva, lung, spleen, trachea, and caecal tonsil samples were collected from both groups at (6, 12, 24, 48 hours, and 5 days after infection) and tested for the presence of NDV using Quantitative Reverse transcription-polymerase Chain Reaction (QRT-PCR). The virus was found in the challenged birds’ spleen as soon as 12 hours after infection, followed by the lungs and trachea. After 2 and 5 days after NDV infection, histologically significant lesions were found, particularly in lymphoid organs. It is concluded that the presence of NDV in Egyptian flocks of chickens could induce major disease in commercial turkeys, necessitating the development of novel vaccinations based on the circulating NDV genotype VII.1.1 in Egypt to protect domestic poultry from recurrent infection. https://advetresearch.com/index.php/AVR/article/view/1046Newcastle disease virus, Turkey poults, virus shedding, RT-PCR, Histological changes |
| spellingShingle | Ahmed M. E. Hegazy Amr Abd El-Fattah Bedair Hala M. N. Tolba Pathogenesis of Isolated Newcastle Disease Virus Genotype VII .1.1 in Turkey Poults in Egypt Journal of Advanced Veterinary Research Newcastle disease virus, Turkey poults, virus shedding, RT-PCR, Histological changes |
| title | Pathogenesis of Isolated Newcastle Disease Virus Genotype VII .1.1 in Turkey Poults in Egypt |
| title_full | Pathogenesis of Isolated Newcastle Disease Virus Genotype VII .1.1 in Turkey Poults in Egypt |
| title_fullStr | Pathogenesis of Isolated Newcastle Disease Virus Genotype VII .1.1 in Turkey Poults in Egypt |
| title_full_unstemmed | Pathogenesis of Isolated Newcastle Disease Virus Genotype VII .1.1 in Turkey Poults in Egypt |
| title_short | Pathogenesis of Isolated Newcastle Disease Virus Genotype VII .1.1 in Turkey Poults in Egypt |
| title_sort | pathogenesis of isolated newcastle disease virus genotype vii 1 1 in turkey poults in egypt |
| topic | Newcastle disease virus, Turkey poults, virus shedding, RT-PCR, Histological changes |
| url | https://advetresearch.com/index.php/AVR/article/view/1046 |
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