Salivary microbiome in patients undergoing hemodialysis and its associations with the duration of the dialysis
Abstract Background Chronic kidney disease (CKD) patients, especially those with end stage renal disease (ESRD) undergoing hemodialysis (HD), exhibit high prevalence of periodontitis. This cross-sectional study aimed to investigate the periodontal status of HD patients and its relationship with sali...
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| Format: | Article |
| Language: | English |
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BMC
2020-09-01
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| Series: | BMC Nephrology |
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| Online Access: | http://link.springer.com/article/10.1186/s12882-020-02009-y |
| _version_ | 1818251068844277760 |
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| author | Xiaobo Duan Xiaolei Chen Megha Gupta Dutmanee Seriwatanachai Hanxiao Xue Qiuchan Xiong Tong Xu Dan Li Anchun Mo Xi Tang Xuedong Zhou Yuqing Li Quan Yuan |
| author_facet | Xiaobo Duan Xiaolei Chen Megha Gupta Dutmanee Seriwatanachai Hanxiao Xue Qiuchan Xiong Tong Xu Dan Li Anchun Mo Xi Tang Xuedong Zhou Yuqing Li Quan Yuan |
| author_sort | Xiaobo Duan |
| collection | DOAJ |
| description | Abstract Background Chronic kidney disease (CKD) patients, especially those with end stage renal disease (ESRD) undergoing hemodialysis (HD), exhibit high prevalence of periodontitis. This cross-sectional study aimed to investigate the periodontal status of HD patients and its relationship with salivary microbiome. Methods One hundred eight HD patients and one hundred healthy control individuals were recruited. They were subjected to periodontal examination followed by saliva samples collection for 16S rRNA gene sequencing. Results The HD patients were with worse periodontal health status, and exhibited higher salivary microbial diversity and lower richness. The periodontal pathogens were significantly enriched in the HD patients. The inferred functional analyze showed microbes enriched in the HD patients were mainly related to metabolism. Despite the periodontal status and overall structure of the microbiome were not significantly altered as the HD duration prolonged, the abundance of Lachnospiraceae [G-2] sp. |HMT_096| is positively correlated with the duration of HD and the community periodontal index (CPI). Five OTUs (operational taxonomic units) belonging to the phyla Firmicutes were enriched as the duration prolonged, and four OTUs originated from the phyla Proteobacteria were negatively related with the CPI index. ESRD patients undergoing HD exhibited microbiota structural, compositional and functional differences compared with the healthy controls. And the species changed as the duration of hemodialysis prolonged. Conclusions End stage renal disease changes salivary microbiome and is a risk factor for oral dysbiosis. |
| first_indexed | 2024-12-12T16:02:25Z |
| format | Article |
| id | doaj.art-cb1876392e6e46908af63ea25d397149 |
| institution | Directory Open Access Journal |
| issn | 1471-2369 |
| language | English |
| last_indexed | 2024-12-12T16:02:25Z |
| publishDate | 2020-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Nephrology |
| spelling | doaj.art-cb1876392e6e46908af63ea25d3971492022-12-22T00:19:23ZengBMCBMC Nephrology1471-23692020-09-0121111110.1186/s12882-020-02009-ySalivary microbiome in patients undergoing hemodialysis and its associations with the duration of the dialysisXiaobo Duan0Xiaolei Chen1Megha Gupta2Dutmanee Seriwatanachai3Hanxiao Xue4Qiuchan Xiong5Tong Xu6Dan Li7Anchun Mo8Xi Tang9Xuedong Zhou10Yuqing Li11Quan Yuan12State Key Laboratory of Oral Diseases & National Clinical Research Centre for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityDepartment of Nephrology, West China Hospital, Sichuan UniversityDepartment of Preventive Dental Sciences, Division of Pedodontics, College of Dentistry, Al-Showajra Academic Campus, Jazan UniversityDepartment of Oral Biology, Faculty of Dentistry, Mahidol UniversityState Key Laboratory of Oral Diseases & National Clinical Research Centre for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityState Key Laboratory of Oral Diseases & National Clinical Research Centre for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityState Key Laboratory of Oral Diseases & National Clinical Research Centre for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityState Key Laboratory of Oral Diseases & National Clinical Research Centre for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityDepartment of Oral Implantology, West China Hospital of Stomatology, Sichuan UniversityDepartment of Nephrology, West China Hospital, Sichuan UniversityState Key Laboratory of Oral Diseases & National Clinical Research Centre for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityState Key Laboratory of Oral Diseases & National Clinical Research Centre for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityState Key Laboratory of Oral Diseases & National Clinical Research Centre for Oral Diseases, West China Hospital of Stomatology, Sichuan UniversityAbstract Background Chronic kidney disease (CKD) patients, especially those with end stage renal disease (ESRD) undergoing hemodialysis (HD), exhibit high prevalence of periodontitis. This cross-sectional study aimed to investigate the periodontal status of HD patients and its relationship with salivary microbiome. Methods One hundred eight HD patients and one hundred healthy control individuals were recruited. They were subjected to periodontal examination followed by saliva samples collection for 16S rRNA gene sequencing. Results The HD patients were with worse periodontal health status, and exhibited higher salivary microbial diversity and lower richness. The periodontal pathogens were significantly enriched in the HD patients. The inferred functional analyze showed microbes enriched in the HD patients were mainly related to metabolism. Despite the periodontal status and overall structure of the microbiome were not significantly altered as the HD duration prolonged, the abundance of Lachnospiraceae [G-2] sp. |HMT_096| is positively correlated with the duration of HD and the community periodontal index (CPI). Five OTUs (operational taxonomic units) belonging to the phyla Firmicutes were enriched as the duration prolonged, and four OTUs originated from the phyla Proteobacteria were negatively related with the CPI index. ESRD patients undergoing HD exhibited microbiota structural, compositional and functional differences compared with the healthy controls. And the species changed as the duration of hemodialysis prolonged. Conclusions End stage renal disease changes salivary microbiome and is a risk factor for oral dysbiosis.http://link.springer.com/article/10.1186/s12882-020-02009-ySalivaOral microbiomeHemodialysisPeriodontal disease |
| spellingShingle | Xiaobo Duan Xiaolei Chen Megha Gupta Dutmanee Seriwatanachai Hanxiao Xue Qiuchan Xiong Tong Xu Dan Li Anchun Mo Xi Tang Xuedong Zhou Yuqing Li Quan Yuan Salivary microbiome in patients undergoing hemodialysis and its associations with the duration of the dialysis BMC Nephrology Saliva Oral microbiome Hemodialysis Periodontal disease |
| title | Salivary microbiome in patients undergoing hemodialysis and its associations with the duration of the dialysis |
| title_full | Salivary microbiome in patients undergoing hemodialysis and its associations with the duration of the dialysis |
| title_fullStr | Salivary microbiome in patients undergoing hemodialysis and its associations with the duration of the dialysis |
| title_full_unstemmed | Salivary microbiome in patients undergoing hemodialysis and its associations with the duration of the dialysis |
| title_short | Salivary microbiome in patients undergoing hemodialysis and its associations with the duration of the dialysis |
| title_sort | salivary microbiome in patients undergoing hemodialysis and its associations with the duration of the dialysis |
| topic | Saliva Oral microbiome Hemodialysis Periodontal disease |
| url | http://link.springer.com/article/10.1186/s12882-020-02009-y |
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