Simultaneous Tests of Theaflavin-3,3′-digallate as an Anti-Diabetic Drug in Human Hepatoma G2 Cells and Zebrafish (<i>Danio rerio</i>)
Theaflavin-3,3′-digallate (TF3) is the most important theaflavin monomer in black tea. TF3 was proved to reduce blood glucose level in mice and rats. However, the elaborate anti-diabetic mechanism was not well elucidated. In this work, human hepatoma G2 (HepG2) cells and zebrafish (<i>Danio re...
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MDPI AG
2021-12-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/12/4379 |
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| author | Hui Zhou Yuanyuan Wu Eunhye Kim Haibo Pan Puming He Bo Li Yi Charlie Chen Youying Tu |
| author_facet | Hui Zhou Yuanyuan Wu Eunhye Kim Haibo Pan Puming He Bo Li Yi Charlie Chen Youying Tu |
| author_sort | Hui Zhou |
| collection | DOAJ |
| description | Theaflavin-3,3′-digallate (TF3) is the most important theaflavin monomer in black tea. TF3 was proved to reduce blood glucose level in mice and rats. However, the elaborate anti-diabetic mechanism was not well elucidated. In this work, human hepatoma G2 (HepG2) cells and zebrafish (<i>Danio rerio</i>) were used simultaneously to reveal anti-diabetic effect of TF3. The results showed that TF3 could effectively rise glucose absorption capacity in insulin-resistant HepG2 cells and regulate glucose level in diabetic zebrafish. The hypoglycemic effect was mediated through down-regulating phosphoenolpyruvate carboxykinase and up-regulating glucokinase. More importantly, TF3 could significantly improve β cells regeneration in diabetic zebrafish at low concentrations (5 μg/mL and 10 μg/mL), which meant TF3 had a strong anti-diabetic effect. Obviously, this work provided the potential benefit of TF3 on hypoglycemic effect, regulating glucose metabolism enzymes, and protecting β cells. TF3 might be a promising agent for combating diabetes. |
| first_indexed | 2024-03-10T03:23:46Z |
| format | Article |
| id | doaj.art-cb1aeb1596884715af160c12635f3259 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T03:23:46Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-cb1aeb1596884715af160c12635f32592023-11-23T09:56:53ZengMDPI AGNutrients2072-66432021-12-011312437910.3390/nu13124379Simultaneous Tests of Theaflavin-3,3′-digallate as an Anti-Diabetic Drug in Human Hepatoma G2 Cells and Zebrafish (<i>Danio rerio</i>)Hui Zhou0Yuanyuan Wu1Eunhye Kim2Haibo Pan3Puming He4Bo Li5Yi Charlie Chen6Youying Tu7Department of Tea Science, Zhejiang University, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, Hangzhou 310058, ChinaDepartment of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, Hangzhou 310058, ChinaDepartment of Tea Science, Zhejiang University, Hangzhou 310058, ChinaCollege of Science, Technology and Mathematics, Alderson Broaddus University, Philippi, WV 26416, USADepartment of Tea Science, Zhejiang University, Hangzhou 310058, ChinaTheaflavin-3,3′-digallate (TF3) is the most important theaflavin monomer in black tea. TF3 was proved to reduce blood glucose level in mice and rats. However, the elaborate anti-diabetic mechanism was not well elucidated. In this work, human hepatoma G2 (HepG2) cells and zebrafish (<i>Danio rerio</i>) were used simultaneously to reveal anti-diabetic effect of TF3. The results showed that TF3 could effectively rise glucose absorption capacity in insulin-resistant HepG2 cells and regulate glucose level in diabetic zebrafish. The hypoglycemic effect was mediated through down-regulating phosphoenolpyruvate carboxykinase and up-regulating glucokinase. More importantly, TF3 could significantly improve β cells regeneration in diabetic zebrafish at low concentrations (5 μg/mL and 10 μg/mL), which meant TF3 had a strong anti-diabetic effect. Obviously, this work provided the potential benefit of TF3 on hypoglycemic effect, regulating glucose metabolism enzymes, and protecting β cells. TF3 might be a promising agent for combating diabetes.https://www.mdpi.com/2072-6643/13/12/4379theaflavin-3,3′-digallateanti-diabetic effectHepG2 cellsdiabetic zebrafish modelglucose metabolism enzymes |
| spellingShingle | Hui Zhou Yuanyuan Wu Eunhye Kim Haibo Pan Puming He Bo Li Yi Charlie Chen Youying Tu Simultaneous Tests of Theaflavin-3,3′-digallate as an Anti-Diabetic Drug in Human Hepatoma G2 Cells and Zebrafish (<i>Danio rerio</i>) Nutrients theaflavin-3,3′-digallate anti-diabetic effect HepG2 cells diabetic zebrafish model glucose metabolism enzymes |
| title | Simultaneous Tests of Theaflavin-3,3′-digallate as an Anti-Diabetic Drug in Human Hepatoma G2 Cells and Zebrafish (<i>Danio rerio</i>) |
| title_full | Simultaneous Tests of Theaflavin-3,3′-digallate as an Anti-Diabetic Drug in Human Hepatoma G2 Cells and Zebrafish (<i>Danio rerio</i>) |
| title_fullStr | Simultaneous Tests of Theaflavin-3,3′-digallate as an Anti-Diabetic Drug in Human Hepatoma G2 Cells and Zebrafish (<i>Danio rerio</i>) |
| title_full_unstemmed | Simultaneous Tests of Theaflavin-3,3′-digallate as an Anti-Diabetic Drug in Human Hepatoma G2 Cells and Zebrafish (<i>Danio rerio</i>) |
| title_short | Simultaneous Tests of Theaflavin-3,3′-digallate as an Anti-Diabetic Drug in Human Hepatoma G2 Cells and Zebrafish (<i>Danio rerio</i>) |
| title_sort | simultaneous tests of theaflavin 3 3 digallate as an anti diabetic drug in human hepatoma g2 cells and zebrafish i danio rerio i |
| topic | theaflavin-3,3′-digallate anti-diabetic effect HepG2 cells diabetic zebrafish model glucose metabolism enzymes |
| url | https://www.mdpi.com/2072-6643/13/12/4379 |
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