Plasticity of cone photoreceptors in adult zebrafish revealed by thyroid hormone exposure
Abstract Vertebrate color vision is predominantly mediated by the presence of multiple cone photoreceptor subtypes that are each maximally sensitive to different wavelengths of light. Thyroid hormone (TH) has been shown to be essential in the spatiotemporal patterning of cone subtypes in many specie...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-42686-x |
| _version_ | 1797576725528313856 |
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| author | Ashley A. Farre Preston Thomas Johnson Huang Rachel A. Poulsen Emmanuel Owusu Poku Deborah L. Stenkamp |
| author_facet | Ashley A. Farre Preston Thomas Johnson Huang Rachel A. Poulsen Emmanuel Owusu Poku Deborah L. Stenkamp |
| author_sort | Ashley A. Farre |
| collection | DOAJ |
| description | Abstract Vertebrate color vision is predominantly mediated by the presence of multiple cone photoreceptor subtypes that are each maximally sensitive to different wavelengths of light. Thyroid hormone (TH) has been shown to be essential in the spatiotemporal patterning of cone subtypes in many species, including cone subtypes that express opsins that are encoded by tandemly replicated genes. TH has been shown to differentially regulate the tandemly replicated lws opsin genes in zebrafish, and exogenous treatments alter the expression levels of these genes in larvae and juveniles. In this study, we sought to determine whether gene expression in cone photoreceptors remains plastic to TH treatment in adults. We used a transgenic lws reporter line, multiplexed fluorescence hybridization chain reaction in situ hybridization, and qPCR to examine the extent to which cone gene expression can be altered by TH in adults. Our studies revealed that opsin gene expression, and the expression of other photoreceptor genes, remains plastic to TH treatment in adult zebrafish. In addition to retinal plasticity, exogenous TH treatment alters skin pigmentation patterns in adult zebrafish after 5 days. Taken together, our results show a remarkable level of TH-sensitive plasticity in the adult zebrafish. |
| first_indexed | 2024-03-10T21:56:53Z |
| format | Article |
| id | doaj.art-cb1b6893592649f898233217f5ceb823 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T21:56:53Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-cb1b6893592649f898233217f5ceb8232023-11-19T13:05:41ZengNature PortfolioScientific Reports2045-23222023-09-0113111410.1038/s41598-023-42686-xPlasticity of cone photoreceptors in adult zebrafish revealed by thyroid hormone exposureAshley A. Farre0Preston Thomas1Johnson Huang2Rachel A. Poulsen3Emmanuel Owusu Poku4Deborah L. Stenkamp5Department of Biological Sciences, University of IdahoWWAMI Medical Education Program, University of Washington School of Medicine, University of IdahoUniversity of Washington School of MedicineBrigham Young University, IdahoDepartment of Biological Sciences, University of IdahoDepartment of Biological Sciences, University of IdahoAbstract Vertebrate color vision is predominantly mediated by the presence of multiple cone photoreceptor subtypes that are each maximally sensitive to different wavelengths of light. Thyroid hormone (TH) has been shown to be essential in the spatiotemporal patterning of cone subtypes in many species, including cone subtypes that express opsins that are encoded by tandemly replicated genes. TH has been shown to differentially regulate the tandemly replicated lws opsin genes in zebrafish, and exogenous treatments alter the expression levels of these genes in larvae and juveniles. In this study, we sought to determine whether gene expression in cone photoreceptors remains plastic to TH treatment in adults. We used a transgenic lws reporter line, multiplexed fluorescence hybridization chain reaction in situ hybridization, and qPCR to examine the extent to which cone gene expression can be altered by TH in adults. Our studies revealed that opsin gene expression, and the expression of other photoreceptor genes, remains plastic to TH treatment in adult zebrafish. In addition to retinal plasticity, exogenous TH treatment alters skin pigmentation patterns in adult zebrafish after 5 days. Taken together, our results show a remarkable level of TH-sensitive plasticity in the adult zebrafish.https://doi.org/10.1038/s41598-023-42686-x |
| spellingShingle | Ashley A. Farre Preston Thomas Johnson Huang Rachel A. Poulsen Emmanuel Owusu Poku Deborah L. Stenkamp Plasticity of cone photoreceptors in adult zebrafish revealed by thyroid hormone exposure Scientific Reports |
| title | Plasticity of cone photoreceptors in adult zebrafish revealed by thyroid hormone exposure |
| title_full | Plasticity of cone photoreceptors in adult zebrafish revealed by thyroid hormone exposure |
| title_fullStr | Plasticity of cone photoreceptors in adult zebrafish revealed by thyroid hormone exposure |
| title_full_unstemmed | Plasticity of cone photoreceptors in adult zebrafish revealed by thyroid hormone exposure |
| title_short | Plasticity of cone photoreceptors in adult zebrafish revealed by thyroid hormone exposure |
| title_sort | plasticity of cone photoreceptors in adult zebrafish revealed by thyroid hormone exposure |
| url | https://doi.org/10.1038/s41598-023-42686-x |
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