Transcriptome Analysis Reveals Roles of Sucrose in Anthocyanin Accumulation in ‘Kuerle Xiangli’ (<i>Pyrus sinkiangensis</i> Yü)
Pear (<i>Pyrus</i> L.) is one of the most important temperate fruit crops worldwide, with considerable economic value and significant health benefits. Red-skinned pears have an attractive appearance and relatively high anthocyanin accumulation, and are especially favored by customers. Ab...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/6/1064 |
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| author | Xiangzhan Zhang Bo Li Ruiwei Duan Chunhong Han Lei Wang Jian Yang Long Wang Suke Wang Yanli Su Huabai Xue |
| author_facet | Xiangzhan Zhang Bo Li Ruiwei Duan Chunhong Han Lei Wang Jian Yang Long Wang Suke Wang Yanli Su Huabai Xue |
| author_sort | Xiangzhan Zhang |
| collection | DOAJ |
| description | Pear (<i>Pyrus</i> L.) is one of the most important temperate fruit crops worldwide, with considerable economic value and significant health benefits. Red-skinned pears have an attractive appearance and relatively high anthocyanin accumulation, and are especially favored by customers. Abnormal weather conditions usually reduce the coloration of red pears. The application of exogenous sucrose obviously promotes anthocyanins accumulation in ‘Kuerle Xiangli’ (<i>Pyrus sinkiangensis</i> Yü); however, the underlying molecular mechanism of sucrose-mediated fruit coloration remains largely unknown. In this study, comprehensive transcriptome analysis was performed to identify the essential regulators and pathways associated with anthocyanin accumulation. The differentially expressed genes enriched in Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes items were analyzed. The transcript levels of some anthocyanin biosynthetic regulatory genes and structural genes were significantly induced by sucrose treatment. Sucrose application also stimulated the expression of some sugar transporter genes. Further RT-qPCR analysis confirmed the induction of anthocyanin biosynthetic genes. Taken together, the results revealed that sucrose promotes pear coloration most likely by regulating sugar metabolism and anthocyanin biosynthesis, and this study provides a comprehensive understanding of the complex molecular mechanisms underlying the coloration of red-skinned pear. |
| first_indexed | 2024-03-09T23:43:13Z |
| format | Article |
| id | doaj.art-cb1f8ef5ac894e94b14a1917757cbb95 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T23:43:13Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-cb1f8ef5ac894e94b14a1917757cbb952023-11-23T16:48:40ZengMDPI AGGenes2073-44252022-06-01136106410.3390/genes13061064Transcriptome Analysis Reveals Roles of Sucrose in Anthocyanin Accumulation in ‘Kuerle Xiangli’ (<i>Pyrus sinkiangensis</i> Yü)Xiangzhan Zhang0Bo Li1Ruiwei Duan2Chunhong Han3Lei Wang4Jian Yang5Long Wang6Suke Wang7Yanli Su8Huabai Xue9Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaZhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaPear (<i>Pyrus</i> L.) is one of the most important temperate fruit crops worldwide, with considerable economic value and significant health benefits. Red-skinned pears have an attractive appearance and relatively high anthocyanin accumulation, and are especially favored by customers. Abnormal weather conditions usually reduce the coloration of red pears. The application of exogenous sucrose obviously promotes anthocyanins accumulation in ‘Kuerle Xiangli’ (<i>Pyrus sinkiangensis</i> Yü); however, the underlying molecular mechanism of sucrose-mediated fruit coloration remains largely unknown. In this study, comprehensive transcriptome analysis was performed to identify the essential regulators and pathways associated with anthocyanin accumulation. The differentially expressed genes enriched in Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes items were analyzed. The transcript levels of some anthocyanin biosynthetic regulatory genes and structural genes were significantly induced by sucrose treatment. Sucrose application also stimulated the expression of some sugar transporter genes. Further RT-qPCR analysis confirmed the induction of anthocyanin biosynthetic genes. Taken together, the results revealed that sucrose promotes pear coloration most likely by regulating sugar metabolism and anthocyanin biosynthesis, and this study provides a comprehensive understanding of the complex molecular mechanisms underlying the coloration of red-skinned pear.https://www.mdpi.com/2073-4425/13/6/1064pearanthocyanin accumulationsucrosecoloration |
| spellingShingle | Xiangzhan Zhang Bo Li Ruiwei Duan Chunhong Han Lei Wang Jian Yang Long Wang Suke Wang Yanli Su Huabai Xue Transcriptome Analysis Reveals Roles of Sucrose in Anthocyanin Accumulation in ‘Kuerle Xiangli’ (<i>Pyrus sinkiangensis</i> Yü) Genes pear anthocyanin accumulation sucrose coloration |
| title | Transcriptome Analysis Reveals Roles of Sucrose in Anthocyanin Accumulation in ‘Kuerle Xiangli’ (<i>Pyrus sinkiangensis</i> Yü) |
| title_full | Transcriptome Analysis Reveals Roles of Sucrose in Anthocyanin Accumulation in ‘Kuerle Xiangli’ (<i>Pyrus sinkiangensis</i> Yü) |
| title_fullStr | Transcriptome Analysis Reveals Roles of Sucrose in Anthocyanin Accumulation in ‘Kuerle Xiangli’ (<i>Pyrus sinkiangensis</i> Yü) |
| title_full_unstemmed | Transcriptome Analysis Reveals Roles of Sucrose in Anthocyanin Accumulation in ‘Kuerle Xiangli’ (<i>Pyrus sinkiangensis</i> Yü) |
| title_short | Transcriptome Analysis Reveals Roles of Sucrose in Anthocyanin Accumulation in ‘Kuerle Xiangli’ (<i>Pyrus sinkiangensis</i> Yü) |
| title_sort | transcriptome analysis reveals roles of sucrose in anthocyanin accumulation in kuerle xiangli i pyrus sinkiangensis i yu |
| topic | pear anthocyanin accumulation sucrose coloration |
| url | https://www.mdpi.com/2073-4425/13/6/1064 |
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