Assessment of Risk Factors in Synanthropic and Wild Rodents Infected by Pathogenic <i>Leptospira</i> spp. Captured in Southern Chile
Leptospirosis is caused by pathogenic <i>Leptospira</i>, and synanthropic and wildlife species of rodents are an important source of infection; however, much of the information about infection progression was obtained from murine models. The aim of this study was to assess infection stat...
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MDPI AG
2020-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/11/2133 |
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| author | Jhuliana Luna Miguel Salgado Carlos Tejeda Manuel Moroni Gustavo Monti |
| author_facet | Jhuliana Luna Miguel Salgado Carlos Tejeda Manuel Moroni Gustavo Monti |
| author_sort | Jhuliana Luna |
| collection | DOAJ |
| description | Leptospirosis is caused by pathogenic <i>Leptospira</i>, and synanthropic and wildlife species of rodents are an important source of infection; however, much of the information about infection progression was obtained from murine models. The aim of this study was to assess infection status and risk factors associated with pathogenic <i>Leptospira</i> in synanthropic and wild rodent species and describe histopathological lesions in several organs from naturally infected animals. In a cross-sectional study, 121 rodents from three synanthropic species and two wild species were trapped in dairy farms in Southern Chile. Liver, heart, kidney, and lungs from trapped animals were fixed in formalin and stained with hematoxylin–eosin. Tissues with lesions consistent with <i>Leptospira</i> infection were tested by immunohistochemistry (IHC) and real-time polymerase chain reaction (qPCR) using the <i>LipL32</i> antigen. Risk factors were assessed by a conditional mixed-logistic regression model. More than half (56.7%) of the negative reactors to the microscopic agglutination test were identified as infected either by IHC/qPCR. A lower risk of infection compared to the rest of the seasons was found in the fall, and the synanthropic species have a lower risk of infection in comparison with the wildlife species. IHC and qPCR contributed to the identification of pathogenic <i>Leptospira</i> in related histological lesions and 50% more infections than serology. |
| first_indexed | 2024-03-10T14:48:07Z |
| format | Article |
| id | doaj.art-cb1fdf11c76546278f3dfc6ace4ff719 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T14:48:07Z |
| publishDate | 2020-11-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-cb1fdf11c76546278f3dfc6ace4ff7192023-11-20T21:13:13ZengMDPI AGAnimals2076-26152020-11-011011213310.3390/ani10112133Assessment of Risk Factors in Synanthropic and Wild Rodents Infected by Pathogenic <i>Leptospira</i> spp. Captured in Southern ChileJhuliana Luna0Miguel Salgado1Carlos Tejeda2Manuel Moroni3Gustavo Monti4Graduate School, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia 5090000, ChilePreventive Veterinary Medicine Institute, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia 5090000, ChilePreventive Veterinary Medicine Institute, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia 5090000, ChileVeterinary Pathology Institute, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia 5090000, ChilePreventive Veterinary Medicine Institute, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia 5090000, ChileLeptospirosis is caused by pathogenic <i>Leptospira</i>, and synanthropic and wildlife species of rodents are an important source of infection; however, much of the information about infection progression was obtained from murine models. The aim of this study was to assess infection status and risk factors associated with pathogenic <i>Leptospira</i> in synanthropic and wild rodent species and describe histopathological lesions in several organs from naturally infected animals. In a cross-sectional study, 121 rodents from three synanthropic species and two wild species were trapped in dairy farms in Southern Chile. Liver, heart, kidney, and lungs from trapped animals were fixed in formalin and stained with hematoxylin–eosin. Tissues with lesions consistent with <i>Leptospira</i> infection were tested by immunohistochemistry (IHC) and real-time polymerase chain reaction (qPCR) using the <i>LipL32</i> antigen. Risk factors were assessed by a conditional mixed-logistic regression model. More than half (56.7%) of the negative reactors to the microscopic agglutination test were identified as infected either by IHC/qPCR. A lower risk of infection compared to the rest of the seasons was found in the fall, and the synanthropic species have a lower risk of infection in comparison with the wildlife species. IHC and qPCR contributed to the identification of pathogenic <i>Leptospira</i> in related histological lesions and 50% more infections than serology.https://www.mdpi.com/2076-2615/10/11/2133leptospirosisrodentsinfection status |
| spellingShingle | Jhuliana Luna Miguel Salgado Carlos Tejeda Manuel Moroni Gustavo Monti Assessment of Risk Factors in Synanthropic and Wild Rodents Infected by Pathogenic <i>Leptospira</i> spp. Captured in Southern Chile Animals leptospirosis rodents infection status |
| title | Assessment of Risk Factors in Synanthropic and Wild Rodents Infected by Pathogenic <i>Leptospira</i> spp. Captured in Southern Chile |
| title_full | Assessment of Risk Factors in Synanthropic and Wild Rodents Infected by Pathogenic <i>Leptospira</i> spp. Captured in Southern Chile |
| title_fullStr | Assessment of Risk Factors in Synanthropic and Wild Rodents Infected by Pathogenic <i>Leptospira</i> spp. Captured in Southern Chile |
| title_full_unstemmed | Assessment of Risk Factors in Synanthropic and Wild Rodents Infected by Pathogenic <i>Leptospira</i> spp. Captured in Southern Chile |
| title_short | Assessment of Risk Factors in Synanthropic and Wild Rodents Infected by Pathogenic <i>Leptospira</i> spp. Captured in Southern Chile |
| title_sort | assessment of risk factors in synanthropic and wild rodents infected by pathogenic i leptospira i spp captured in southern chile |
| topic | leptospirosis rodents infection status |
| url | https://www.mdpi.com/2076-2615/10/11/2133 |
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