Nigella sativa seed decreases endothelial dysfunction in streptozotocin-induced diabetic rat aorta
Objective: Diabetes is an important risk factor for cardiovascular events. The great percent of morbidity in patients with diabetes is due to endothelial dysfunction. The present study investigated the effects of hydroalcholic extract of Nigella sativa (N. sativa) on contractile and dilatation resp...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2016-01-01
|
| Series: | Avicenna Journal of Phytomedicine |
| Subjects: | |
| Online Access: | http://ajp.mums.ac.ir/pdf_4187_31003b0cd75a46747be9f1d5e5b35f62.html |
| _version_ | 1818843778721513472 |
|---|---|
| author | Abbasali Abbasnezhad Saeed Niazmand Maryam Mahmoudabady Mohammad Soukhtanloo Seyed Abdolrahim Rezaee Seyed Mojtaba Mousavi |
| author_facet | Abbasali Abbasnezhad Saeed Niazmand Maryam Mahmoudabady Mohammad Soukhtanloo Seyed Abdolrahim Rezaee Seyed Mojtaba Mousavi |
| author_sort | Abbasali Abbasnezhad |
| collection | DOAJ |
| description | Objective: Diabetes is an important risk factor for cardiovascular events. The great percent of morbidity in patients with diabetes is due to endothelial dysfunction. The present study investigated the effects of hydroalcholic extract of Nigella sativa (N. sativa) on contractile and dilatation response of isolated aorta in streptozotocin (STZ)-induced diabetic rat. Materials and Methods: Rats were divided into six experimental groups (control, untreated STZ-diabetic, and N. sativa hydroalcholic extract or metformin-treated diabetic rats). Treated rats received N. sativa extract (100, 200, and 400 mg/kg) or metformin (300 mg/kg) by gavage, daily for 6 weeks. Isolated rat thoracic rings were mounted in an organ bath system then contractile and dilatation responses induced by phenylephrine (PE), acetylcholine (ACh), potassium chloride (KCl), and sodium nitroprusside (SNP) were evaluated in different situations. Results: The lower concentrations of N. sativa seed extract (DE 100 and DE 200) and metformin significantly reduced the contractile responses to higher concentrations of PE (10-6 - 10-5 M) compared to diabetic group (pThe relaxation response to Ach 10-8M, was increased in DE 200 and metformin groups compared to diabetic group (p |
| first_indexed | 2024-12-19T05:03:17Z |
| format | Article |
| id | doaj.art-cb2135395f43462b87f423d5b36a5ee7 |
| institution | Directory Open Access Journal |
| issn | 2228-7930 2228-7949 |
| language | English |
| last_indexed | 2024-12-19T05:03:17Z |
| publishDate | 2016-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Phytomedicine |
| spelling | doaj.art-cb2135395f43462b87f423d5b36a5ee72022-12-21T20:35:01ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492016-01-016167764187Nigella sativa seed decreases endothelial dysfunction in streptozotocin-induced diabetic rat aortaAbbasali Abbasnezhad0Saeed Niazmand1Maryam Mahmoudabady2Mohammad Soukhtanloo3Seyed Abdolrahim Rezaee4Seyed Mojtaba Mousavi5Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranCardiovascular Research Center, Mashhad University of Medical Sciences, Mashhad, IranNeurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Immunology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranObjective: Diabetes is an important risk factor for cardiovascular events. The great percent of morbidity in patients with diabetes is due to endothelial dysfunction. The present study investigated the effects of hydroalcholic extract of Nigella sativa (N. sativa) on contractile and dilatation response of isolated aorta in streptozotocin (STZ)-induced diabetic rat. Materials and Methods: Rats were divided into six experimental groups (control, untreated STZ-diabetic, and N. sativa hydroalcholic extract or metformin-treated diabetic rats). Treated rats received N. sativa extract (100, 200, and 400 mg/kg) or metformin (300 mg/kg) by gavage, daily for 6 weeks. Isolated rat thoracic rings were mounted in an organ bath system then contractile and dilatation responses induced by phenylephrine (PE), acetylcholine (ACh), potassium chloride (KCl), and sodium nitroprusside (SNP) were evaluated in different situations. Results: The lower concentrations of N. sativa seed extract (DE 100 and DE 200) and metformin significantly reduced the contractile responses to higher concentrations of PE (10-6 - 10-5 M) compared to diabetic group (pThe relaxation response to Ach 10-8M, was increased in DE 200 and metformin groups compared to diabetic group (phttp://ajp.mums.ac.ir/pdf_4187_31003b0cd75a46747be9f1d5e5b35f62.htmlDiabetes MellitusNigella sativaEndothelial dysfunctionIsolated aortaRat |
| spellingShingle | Abbasali Abbasnezhad Saeed Niazmand Maryam Mahmoudabady Mohammad Soukhtanloo Seyed Abdolrahim Rezaee Seyed Mojtaba Mousavi Nigella sativa seed decreases endothelial dysfunction in streptozotocin-induced diabetic rat aorta Avicenna Journal of Phytomedicine Diabetes Mellitus Nigella sativa Endothelial dysfunction Isolated aorta Rat |
| title | Nigella sativa seed decreases endothelial dysfunction in streptozotocin-induced diabetic rat aorta |
| title_full | Nigella sativa seed decreases endothelial dysfunction in streptozotocin-induced diabetic rat aorta |
| title_fullStr | Nigella sativa seed decreases endothelial dysfunction in streptozotocin-induced diabetic rat aorta |
| title_full_unstemmed | Nigella sativa seed decreases endothelial dysfunction in streptozotocin-induced diabetic rat aorta |
| title_short | Nigella sativa seed decreases endothelial dysfunction in streptozotocin-induced diabetic rat aorta |
| title_sort | nigella sativa seed decreases endothelial dysfunction in streptozotocin induced diabetic rat aorta |
| topic | Diabetes Mellitus Nigella sativa Endothelial dysfunction Isolated aorta Rat |
| url | http://ajp.mums.ac.ir/pdf_4187_31003b0cd75a46747be9f1d5e5b35f62.html |
| work_keys_str_mv | AT abbasaliabbasnezhad nigellasativaseeddecreasesendothelialdysfunctioninstreptozotocininduceddiabeticrataorta AT saeedniazmand nigellasativaseeddecreasesendothelialdysfunctioninstreptozotocininduceddiabeticrataorta AT maryammahmoudabady nigellasativaseeddecreasesendothelialdysfunctioninstreptozotocininduceddiabeticrataorta AT mohammadsoukhtanloo nigellasativaseeddecreasesendothelialdysfunctioninstreptozotocininduceddiabeticrataorta AT seyedabdolrahimrezaee nigellasativaseeddecreasesendothelialdysfunctioninstreptozotocininduceddiabeticrataorta AT seyedmojtabamousavi nigellasativaseeddecreasesendothelialdysfunctioninstreptozotocininduceddiabeticrataorta |