Cell Lysis, labeling efficacy and biodistribution of intravenously administered Technetium-99 m labeled multifunctional zirconia nanoparticles in animal model
Nanoparticles, to be used in biomedical applications, have been the subject of extensive investigation in recent years. Recently, zirconia and iron have gained significant relevance to the field due to their superior mechanical and structural qualities as well as their numerous biological applicatio...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Arabian Journal of Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535223007293 |
| _version_ | 1797615950901542912 |
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| author | Ifra SanaUllah Saira Riaz Irfan Ullah Khan Daoud Ali S. Shamaila M. Akram Raza Amna Sajjad Anjum N. Sabri Shahzad Naseem |
| author_facet | Ifra SanaUllah Saira Riaz Irfan Ullah Khan Daoud Ali S. Shamaila M. Akram Raza Amna Sajjad Anjum N. Sabri Shahzad Naseem |
| author_sort | Ifra SanaUllah |
| collection | DOAJ |
| description | Nanoparticles, to be used in biomedical applications, have been the subject of extensive investigation in recent years. Recently, zirconia and iron have gained significant relevance to the field due to their superior mechanical and structural qualities as well as their numerous biological applications. In this research, Fe3O4-stabilized zirconia nanopowders are fabricated using a low-cost sol–gel approach. Iron oxide sol is added in 1–10 wt% increments to a 0.1 M zirconia sol. The obtained crystallite size values match well with previously reported values for the tetragonally stable (t-zirconia) phase. Hardness values of approximately 1278 HV, along with fracture toughness values of ∼ 24.48 MPa.m-1/2 are observed for stable tetragonal zirconia. Results from cell lysis experiments indicate that the synthesized nanoparticles have the potential to combat cancerous cells. An encapsulation efficacy of up to ∼ 73% is observed after a 120-minute time period. Radio labeling techniques are employed to label the as-synthesized zirconia nanoparticles using Sodium Pertechnetate (Na99mTcO4) labeled radiopharmaceuticals, allowing for biodistribution assessment. Multiple CT scans of rabbits are performed to obtain accurate results and evaluate their survival. Studies show that radiolabeled nanoparticles are highly absorbed in the animals' bladders, making them a promising choice for tumor therapy. Various physical properties of the animals are periodically examined after administration of the nanoparticle-based injection for several months, and no abnormalities are found. The results obtained through these characterizations indicate that these nanoparticles can be used as future therapeutic agents as well as drug carriers. |
| first_indexed | 2024-03-11T07:34:15Z |
| format | Article |
| id | doaj.art-cb28785e53804d4bbd4bf41e1d3e180a |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-11T07:34:15Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-cb28785e53804d4bbd4bf41e1d3e180a2023-11-17T05:25:49ZengElsevierArabian Journal of Chemistry1878-53522023-12-011612105267Cell Lysis, labeling efficacy and biodistribution of intravenously administered Technetium-99 m labeled multifunctional zirconia nanoparticles in animal modelIfra SanaUllah0Saira Riaz1Irfan Ullah Khan2Daoud Ali3S. Shamaila4M. Akram Raza5Amna Sajjad6Anjum N. Sabri7Shahzad Naseem8Centre of Excellence in Solid State Physics, University of the Punjab, QAC, Lahore 54590, PakistanCentre of Excellence in Solid State Physics, University of the Punjab, QAC, Lahore 54590, Pakistan; Corresponding authors.Department of Cyclotron and Allied Radiopharmaceutics, INMOL Cancer Hospital, Lahore 54600, PakistanDepartment of Zoology, College of Science, King Saud University, Riyadh 11451, Saudia ArabiaWaterloo Institute for Nanotechnology, University of Waterloo, Ontario, CanadaCentre of Excellence in Solid State Physics, University of the Punjab, QAC, Lahore 54590, PakistanDepartment of Zoology, Government College University, Faisalabad, PakistanDepartment of Microbiology and Molecular Genetics, University of the Punjab, QAC, Lahore 54590, PakistanCentre of Excellence in Solid State Physics, University of the Punjab, QAC, Lahore 54590, Pakistan; Corresponding authors.Nanoparticles, to be used in biomedical applications, have been the subject of extensive investigation in recent years. Recently, zirconia and iron have gained significant relevance to the field due to their superior mechanical and structural qualities as well as their numerous biological applications. In this research, Fe3O4-stabilized zirconia nanopowders are fabricated using a low-cost sol–gel approach. Iron oxide sol is added in 1–10 wt% increments to a 0.1 M zirconia sol. The obtained crystallite size values match well with previously reported values for the tetragonally stable (t-zirconia) phase. Hardness values of approximately 1278 HV, along with fracture toughness values of ∼ 24.48 MPa.m-1/2 are observed for stable tetragonal zirconia. Results from cell lysis experiments indicate that the synthesized nanoparticles have the potential to combat cancerous cells. An encapsulation efficacy of up to ∼ 73% is observed after a 120-minute time period. Radio labeling techniques are employed to label the as-synthesized zirconia nanoparticles using Sodium Pertechnetate (Na99mTcO4) labeled radiopharmaceuticals, allowing for biodistribution assessment. Multiple CT scans of rabbits are performed to obtain accurate results and evaluate their survival. Studies show that radiolabeled nanoparticles are highly absorbed in the animals' bladders, making them a promising choice for tumor therapy. Various physical properties of the animals are periodically examined after administration of the nanoparticle-based injection for several months, and no abnormalities are found. The results obtained through these characterizations indicate that these nanoparticles can be used as future therapeutic agents as well as drug carriers.http://www.sciencedirect.com/science/article/pii/S1878535223007293Iron oxideZirconiaHoneyAnticancerDrug loading |
| spellingShingle | Ifra SanaUllah Saira Riaz Irfan Ullah Khan Daoud Ali S. Shamaila M. Akram Raza Amna Sajjad Anjum N. Sabri Shahzad Naseem Cell Lysis, labeling efficacy and biodistribution of intravenously administered Technetium-99 m labeled multifunctional zirconia nanoparticles in animal model Arabian Journal of Chemistry Iron oxide Zirconia Honey Anticancer Drug loading |
| title | Cell Lysis, labeling efficacy and biodistribution of intravenously administered Technetium-99 m labeled multifunctional zirconia nanoparticles in animal model |
| title_full | Cell Lysis, labeling efficacy and biodistribution of intravenously administered Technetium-99 m labeled multifunctional zirconia nanoparticles in animal model |
| title_fullStr | Cell Lysis, labeling efficacy and biodistribution of intravenously administered Technetium-99 m labeled multifunctional zirconia nanoparticles in animal model |
| title_full_unstemmed | Cell Lysis, labeling efficacy and biodistribution of intravenously administered Technetium-99 m labeled multifunctional zirconia nanoparticles in animal model |
| title_short | Cell Lysis, labeling efficacy and biodistribution of intravenously administered Technetium-99 m labeled multifunctional zirconia nanoparticles in animal model |
| title_sort | cell lysis labeling efficacy and biodistribution of intravenously administered technetium 99 m labeled multifunctional zirconia nanoparticles in animal model |
| topic | Iron oxide Zirconia Honey Anticancer Drug loading |
| url | http://www.sciencedirect.com/science/article/pii/S1878535223007293 |
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