Healing Capacity of Bone Surrounding Biofilm-Infected and Non-Infected Gutta-Percha: A Study of Rat Calvaria
This paper aims to evaluate the healing capacity of bony lesions around biofilm-infected and non-infected gutta-percha (GP) points. Bony defects were created in the calvaria of 28 Wistar rats. The rats were divided into three groups: Group 1—Implantation of infected GP particles in the bony defect;...
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MDPI AG
2021-07-01
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| Online Access: | https://www.mdpi.com/2076-3417/11/15/6710 |
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| author | Daniel Moreinos Ronald Wigler Yuval Geffen Sharon Akrish Shaul Lin |
| author_facet | Daniel Moreinos Ronald Wigler Yuval Geffen Sharon Akrish Shaul Lin |
| author_sort | Daniel Moreinos |
| collection | DOAJ |
| description | This paper aims to evaluate the healing capacity of bony lesions around biofilm-infected and non-infected gutta-percha (GP) points. Bony defects were created in the calvaria of 28 Wistar rats. The rats were divided into three groups: Group 1—Implantation of infected GP particles in the bony defect; Group 2—Positive control implantation of non-infected GP particles in the bony defect; and Group 3—Negative control, in which no GP particles were implanted. The biofilm consisted of three strains of bacteria: Enterococcus faecalis, Streptococcus sanguis, and Porphyromonas gingivalis. The animals were sacrificed 60 days postoperation, and histological assessments were performed. In Group 1, the biofilm-infected group, we observed a mild foreign body reaction with a few inflammatory cells adjacent to the capsule and a newly woven bone matrix surrounded by osteoblasts and mature bone. In Group 2, the non-infected GP particles group, minimal inflammatory cell reactions were observed in the adjacent tissue, and a newly woven bone matrix was surrounded by osteoblasts. This study shows that bone healing is possible around both sterile and infected GP points. This contradicts the claim that some root canal treatments fail because of non-microbial factors, including extruded root canal filling materials, which may cause foreign body reactions. The healing observed suggests that overextension should not be considered an indication for endodontic surgery. |
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| id | doaj.art-cb2b1ae988c04956b813be843c02bab0 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T09:20:13Z |
| publishDate | 2021-07-01 |
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| record_format | Article |
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| spelling | doaj.art-cb2b1ae988c04956b813be843c02bab02023-11-22T05:18:19ZengMDPI AGApplied Sciences2076-34172021-07-011115671010.3390/app11156710Healing Capacity of Bone Surrounding Biofilm-Infected and Non-Infected Gutta-Percha: A Study of Rat CalvariaDaniel Moreinos0Ronald Wigler1Yuval Geffen2Sharon Akrish3Shaul Lin4Endodontics and Dental Trauma Department, School of Graduate Dentistry, Rambam Health Care Campus, P.O. Box 9602, Haifa 3109601, IsraelDepartment of Endodontology, The Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv 6100000, IsraelDepartment of Pathology, Rambam Health Care Campus, Haifa 3500000, IsraelClinical Microbiology Laboratory, Rambam Health Care Campus, Haifa 3500000, IsraelEndodontics and Dental Trauma Department, School of Graduate Dentistry, Rambam Health Care Campus, P.O. Box 9602, Haifa 3109601, IsraelThis paper aims to evaluate the healing capacity of bony lesions around biofilm-infected and non-infected gutta-percha (GP) points. Bony defects were created in the calvaria of 28 Wistar rats. The rats were divided into three groups: Group 1—Implantation of infected GP particles in the bony defect; Group 2—Positive control implantation of non-infected GP particles in the bony defect; and Group 3—Negative control, in which no GP particles were implanted. The biofilm consisted of three strains of bacteria: Enterococcus faecalis, Streptococcus sanguis, and Porphyromonas gingivalis. The animals were sacrificed 60 days postoperation, and histological assessments were performed. In Group 1, the biofilm-infected group, we observed a mild foreign body reaction with a few inflammatory cells adjacent to the capsule and a newly woven bone matrix surrounded by osteoblasts and mature bone. In Group 2, the non-infected GP particles group, minimal inflammatory cell reactions were observed in the adjacent tissue, and a newly woven bone matrix was surrounded by osteoblasts. This study shows that bone healing is possible around both sterile and infected GP points. This contradicts the claim that some root canal treatments fail because of non-microbial factors, including extruded root canal filling materials, which may cause foreign body reactions. The healing observed suggests that overextension should not be considered an indication for endodontic surgery.https://www.mdpi.com/2076-3417/11/15/6710biofilmbone healing capacityinfected gutta-percharat calvarium |
| spellingShingle | Daniel Moreinos Ronald Wigler Yuval Geffen Sharon Akrish Shaul Lin Healing Capacity of Bone Surrounding Biofilm-Infected and Non-Infected Gutta-Percha: A Study of Rat Calvaria Applied Sciences biofilm bone healing capacity infected gutta-percha rat calvarium |
| title | Healing Capacity of Bone Surrounding Biofilm-Infected and Non-Infected Gutta-Percha: A Study of Rat Calvaria |
| title_full | Healing Capacity of Bone Surrounding Biofilm-Infected and Non-Infected Gutta-Percha: A Study of Rat Calvaria |
| title_fullStr | Healing Capacity of Bone Surrounding Biofilm-Infected and Non-Infected Gutta-Percha: A Study of Rat Calvaria |
| title_full_unstemmed | Healing Capacity of Bone Surrounding Biofilm-Infected and Non-Infected Gutta-Percha: A Study of Rat Calvaria |
| title_short | Healing Capacity of Bone Surrounding Biofilm-Infected and Non-Infected Gutta-Percha: A Study of Rat Calvaria |
| title_sort | healing capacity of bone surrounding biofilm infected and non infected gutta percha a study of rat calvaria |
| topic | biofilm bone healing capacity infected gutta-percha rat calvarium |
| url | https://www.mdpi.com/2076-3417/11/15/6710 |
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