Seroprevalence of neutralizing antibodies against adenovirus type 14 and 55 in healthy adults in Southern China<subtitle>Prevalence of Ad14 and 55-neutralizing antibodies</subtitle>
Re-emerging human adenovirus types 14 (Ad14) and 55 (Ad55) have caused severe respiratory diseases and even deaths during recent outbreaks. However, the seroprevalence of neutralizing antibodies (nAbs) in healthy adults, which may reflect previous circulation and help to predict potential outbreaks,...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2017-01-01
|
| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1038/emi.2017.29 |
| _version_ | 1797676906820141056 |
|---|---|
| author | Xuehua Zheng Xia Rong Ying Feng Xikui Sun Liang Li Qian Wang Min Wang Wenkuan Liu Chufang Li Yiyu Yang Rong Zhou Jiahai Lu Liqiang Feng Ling Chen |
| author_facet | Xuehua Zheng Xia Rong Ying Feng Xikui Sun Liang Li Qian Wang Min Wang Wenkuan Liu Chufang Li Yiyu Yang Rong Zhou Jiahai Lu Liqiang Feng Ling Chen |
| author_sort | Xuehua Zheng |
| collection | DOAJ |
| description | Re-emerging human adenovirus types 14 (Ad14) and 55 (Ad55) have caused severe respiratory diseases and even deaths during recent outbreaks. However, the seroprevalence of neutralizing antibodies (nAbs) in healthy adults, which may reflect previous circulation and help to predict potential outbreaks, remains unclear. In this study, we established micro-neutralizing (MN) assays on the basis of recombinant Ad14 and Ad55 reporter viruses, and we investigated serum nAbs in healthy blood donors from Southern China. We found that the overall seropositive rates were 24.8% and 22.4% for Ad14 and Ad55 nAbs, respectively. The seropositive rates were low in individuals younger than 20, and they gradually increased with age. Ad55-seropositive individuals tended to have high nAb titers (>1000), while low (72–200) and moderate (201–1000) nAb levels were frequently observed in Ad14-seropositive ones. Surprisingly, the seropositive rates and nAb levels were associated with the blood type but not the gender of the blood donors, with type AB individuals displaying higher seropositive rates and nAb levels. Interestingly, a significant positive correlation was observed between Ad14 and Ad55 seroprevalence, and higher titers of nAbs were detected in double-positive individuals compared to single-positive ones. These results clarified the human humoral immune responses against Ad14 and Ad55 and revealed a low level of herd immunity in some subpopulations, which emphasized the importance of monitoring these two highly virulent adenoviruses and reinforced the development of prophylactic vaccines. |
| first_indexed | 2024-03-11T22:37:15Z |
| format | Article |
| id | doaj.art-cb2c9590e50a4d208f564a80158131cd |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-03-11T22:37:15Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-cb2c9590e50a4d208f564a80158131cd2023-09-22T12:08:35ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512017-01-01611810.1038/emi.2017.29Seroprevalence of neutralizing antibodies against adenovirus type 14 and 55 in healthy adults in Southern China<subtitle>Prevalence of Ad14 and 55-neutralizing antibodies</subtitle>Xuehua Zheng0Xia Rong1Ying Feng2Xikui Sun3Liang Li4Qian Wang5Min Wang6Wenkuan Liu7Chufang Li8Yiyu Yang9Rong Zhou10Jiahai Lu11Liqiang Feng12Ling Chen13State Key Laboratory of Respiratory Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, ChinaInstitute of Clinical Blood Transfusion, Guangzhou Blood Center, Guangzhou 510095, ChinaState Key Laboratory of Respiratory Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, ChinaState Key Laboratory of Respiratory Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, ChinaState Key Laboratory of Respiratory Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, ChinaState Key Laboratory of Respiratory Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, ChinaInstitute of Clinical Blood Transfusion, Guangzhou Blood Center, Guangzhou 510095, ChinaThe First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510230, ChinaThe First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510230, ChinaPediatric Intensive Care Unit, Guangzhou Women and Children’s Medical Center, Guangzhou 510180, ChinaThe First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510230, ChinaDepartment of Medical Statistics and Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou 510080, ChinaState Key Laboratory of Respiratory Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, ChinaState Key Laboratory of Respiratory Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, ChinaRe-emerging human adenovirus types 14 (Ad14) and 55 (Ad55) have caused severe respiratory diseases and even deaths during recent outbreaks. However, the seroprevalence of neutralizing antibodies (nAbs) in healthy adults, which may reflect previous circulation and help to predict potential outbreaks, remains unclear. In this study, we established micro-neutralizing (MN) assays on the basis of recombinant Ad14 and Ad55 reporter viruses, and we investigated serum nAbs in healthy blood donors from Southern China. We found that the overall seropositive rates were 24.8% and 22.4% for Ad14 and Ad55 nAbs, respectively. The seropositive rates were low in individuals younger than 20, and they gradually increased with age. Ad55-seropositive individuals tended to have high nAb titers (>1000), while low (72–200) and moderate (201–1000) nAb levels were frequently observed in Ad14-seropositive ones. Surprisingly, the seropositive rates and nAb levels were associated with the blood type but not the gender of the blood donors, with type AB individuals displaying higher seropositive rates and nAb levels. Interestingly, a significant positive correlation was observed between Ad14 and Ad55 seroprevalence, and higher titers of nAbs were detected in double-positive individuals compared to single-positive ones. These results clarified the human humoral immune responses against Ad14 and Ad55 and revealed a low level of herd immunity in some subpopulations, which emphasized the importance of monitoring these two highly virulent adenoviruses and reinforced the development of prophylactic vaccines.https://www.tandfonline.com/doi/10.1038/emi.2017.29human adenovirus type 14human adenovirus type 55neutralizing antibodyseroprevalenceSouthern China |
| spellingShingle | Xuehua Zheng Xia Rong Ying Feng Xikui Sun Liang Li Qian Wang Min Wang Wenkuan Liu Chufang Li Yiyu Yang Rong Zhou Jiahai Lu Liqiang Feng Ling Chen Seroprevalence of neutralizing antibodies against adenovirus type 14 and 55 in healthy adults in Southern China<subtitle>Prevalence of Ad14 and 55-neutralizing antibodies</subtitle> Emerging Microbes and Infections human adenovirus type 14 human adenovirus type 55 neutralizing antibody seroprevalence Southern China |
| title | Seroprevalence of neutralizing antibodies against adenovirus type 14 and 55 in healthy adults in Southern China<subtitle>Prevalence of Ad14 and 55-neutralizing antibodies</subtitle> |
| title_full | Seroprevalence of neutralizing antibodies against adenovirus type 14 and 55 in healthy adults in Southern China<subtitle>Prevalence of Ad14 and 55-neutralizing antibodies</subtitle> |
| title_fullStr | Seroprevalence of neutralizing antibodies against adenovirus type 14 and 55 in healthy adults in Southern China<subtitle>Prevalence of Ad14 and 55-neutralizing antibodies</subtitle> |
| title_full_unstemmed | Seroprevalence of neutralizing antibodies against adenovirus type 14 and 55 in healthy adults in Southern China<subtitle>Prevalence of Ad14 and 55-neutralizing antibodies</subtitle> |
| title_short | Seroprevalence of neutralizing antibodies against adenovirus type 14 and 55 in healthy adults in Southern China<subtitle>Prevalence of Ad14 and 55-neutralizing antibodies</subtitle> |
| title_sort | seroprevalence of neutralizing antibodies against adenovirus type 14 and 55 in healthy adults in southern china subtitle prevalence of ad14 and 55 neutralizing antibodies subtitle |
| topic | human adenovirus type 14 human adenovirus type 55 neutralizing antibody seroprevalence Southern China |
| url | https://www.tandfonline.com/doi/10.1038/emi.2017.29 |
| work_keys_str_mv | AT xuehuazheng seroprevalenceofneutralizingantibodiesagainstadenovirustype14and55inhealthyadultsinsouthernchinasubtitleprevalenceofad14and55neutralizingantibodiessubtitle AT xiarong seroprevalenceofneutralizingantibodiesagainstadenovirustype14and55inhealthyadultsinsouthernchinasubtitleprevalenceofad14and55neutralizingantibodiessubtitle AT yingfeng seroprevalenceofneutralizingantibodiesagainstadenovirustype14and55inhealthyadultsinsouthernchinasubtitleprevalenceofad14and55neutralizingantibodiessubtitle AT xikuisun seroprevalenceofneutralizingantibodiesagainstadenovirustype14and55inhealthyadultsinsouthernchinasubtitleprevalenceofad14and55neutralizingantibodiessubtitle AT liangli seroprevalenceofneutralizingantibodiesagainstadenovirustype14and55inhealthyadultsinsouthernchinasubtitleprevalenceofad14and55neutralizingantibodiessubtitle AT qianwang seroprevalenceofneutralizingantibodiesagainstadenovirustype14and55inhealthyadultsinsouthernchinasubtitleprevalenceofad14and55neutralizingantibodiessubtitle AT minwang seroprevalenceofneutralizingantibodiesagainstadenovirustype14and55inhealthyadultsinsouthernchinasubtitleprevalenceofad14and55neutralizingantibodiessubtitle AT wenkuanliu seroprevalenceofneutralizingantibodiesagainstadenovirustype14and55inhealthyadultsinsouthernchinasubtitleprevalenceofad14and55neutralizingantibodiessubtitle AT chufangli seroprevalenceofneutralizingantibodiesagainstadenovirustype14and55inhealthyadultsinsouthernchinasubtitleprevalenceofad14and55neutralizingantibodiessubtitle AT yiyuyang seroprevalenceofneutralizingantibodiesagainstadenovirustype14and55inhealthyadultsinsouthernchinasubtitleprevalenceofad14and55neutralizingantibodiessubtitle AT rongzhou seroprevalenceofneutralizingantibodiesagainstadenovirustype14and55inhealthyadultsinsouthernchinasubtitleprevalenceofad14and55neutralizingantibodiessubtitle AT jiahailu seroprevalenceofneutralizingantibodiesagainstadenovirustype14and55inhealthyadultsinsouthernchinasubtitleprevalenceofad14and55neutralizingantibodiessubtitle AT liqiangfeng seroprevalenceofneutralizingantibodiesagainstadenovirustype14and55inhealthyadultsinsouthernchinasubtitleprevalenceofad14and55neutralizingantibodiessubtitle AT lingchen seroprevalenceofneutralizingantibodiesagainstadenovirustype14and55inhealthyadultsinsouthernchinasubtitleprevalenceofad14and55neutralizingantibodiessubtitle |