Functional and Metabolic Characterization of Microglia Culture in a Defined Medium
Microglia are the endogenous immune cells of the brain and act as sensor of infection and pathologic injury to the brain, leading to a rapid plastic process of activation that culminates in the endocytosis and phagocytosis of damaged tissue. Microglia cells are the most plastic cells in the brain. M...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-02-01
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| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fncel.2020.00022/full |
| _version_ | 1811215020391923712 |
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| author | Alejandro Montilla Alejandro Montilla Alejandro Montilla Alazne Zabala Alazne Zabala Alazne Zabala Carlos Matute Carlos Matute Carlos Matute María Domercq María Domercq María Domercq |
| author_facet | Alejandro Montilla Alejandro Montilla Alejandro Montilla Alazne Zabala Alazne Zabala Alazne Zabala Carlos Matute Carlos Matute Carlos Matute María Domercq María Domercq María Domercq |
| author_sort | Alejandro Montilla |
| collection | DOAJ |
| description | Microglia are the endogenous immune cells of the brain and act as sensor of infection and pathologic injury to the brain, leading to a rapid plastic process of activation that culminates in the endocytosis and phagocytosis of damaged tissue. Microglia cells are the most plastic cells in the brain. Microglia isolation from their environment as well as culturing them in the presence of serum alter their function and lead to a rapid loss of their signature gene expression. Previous studies have identified pivotal factors allowing microglia culture in the absence of serum. Here, we have further characterized the function, expression of markers, metabolic status and response to pro and anti-inflammatory stimulus of microglia isolated by magnetic-activated cell sorting and cultured in a chemically defined medium. We have compared this new method with previous traditional protocols of culturing microglia that use high concentrations of serum. |
| first_indexed | 2024-04-12T06:15:17Z |
| format | Article |
| id | doaj.art-cb2ea2303efd4549b232d3bf2af2f5b9 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-04-12T06:15:17Z |
| publishDate | 2020-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-cb2ea2303efd4549b232d3bf2af2f5b92022-12-22T03:44:32ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022020-02-011410.3389/fncel.2020.00022506355Functional and Metabolic Characterization of Microglia Culture in a Defined MediumAlejandro Montilla0Alejandro Montilla1Alejandro Montilla2Alazne Zabala3Alazne Zabala4Alazne Zabala5Carlos Matute6Carlos Matute7Carlos Matute8María Domercq9María Domercq10María Domercq11Department of Neurosciences, University of the Basque Country, Leioa, SpainAchucarro Basque Center for Neuroscience-UPV/EHU, Leioa, SpainInstituto de Salud Carlos III, Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED), Leioa, SpainDepartment of Neurosciences, University of the Basque Country, Leioa, SpainAchucarro Basque Center for Neuroscience-UPV/EHU, Leioa, SpainInstituto de Salud Carlos III, Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED), Leioa, SpainDepartment of Neurosciences, University of the Basque Country, Leioa, SpainAchucarro Basque Center for Neuroscience-UPV/EHU, Leioa, SpainInstituto de Salud Carlos III, Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED), Leioa, SpainDepartment of Neurosciences, University of the Basque Country, Leioa, SpainAchucarro Basque Center for Neuroscience-UPV/EHU, Leioa, SpainInstituto de Salud Carlos III, Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED), Leioa, SpainMicroglia are the endogenous immune cells of the brain and act as sensor of infection and pathologic injury to the brain, leading to a rapid plastic process of activation that culminates in the endocytosis and phagocytosis of damaged tissue. Microglia cells are the most plastic cells in the brain. Microglia isolation from their environment as well as culturing them in the presence of serum alter their function and lead to a rapid loss of their signature gene expression. Previous studies have identified pivotal factors allowing microglia culture in the absence of serum. Here, we have further characterized the function, expression of markers, metabolic status and response to pro and anti-inflammatory stimulus of microglia isolated by magnetic-activated cell sorting and cultured in a chemically defined medium. We have compared this new method with previous traditional protocols of culturing microglia that use high concentrations of serum.https://www.frontiersin.org/article/10.3389/fncel.2020.00022/fullmicrogliacultureramificationphagocytosismetabolic reprogrammingpurinergic receptors |
| spellingShingle | Alejandro Montilla Alejandro Montilla Alejandro Montilla Alazne Zabala Alazne Zabala Alazne Zabala Carlos Matute Carlos Matute Carlos Matute María Domercq María Domercq María Domercq Functional and Metabolic Characterization of Microglia Culture in a Defined Medium Frontiers in Cellular Neuroscience microglia culture ramification phagocytosis metabolic reprogramming purinergic receptors |
| title | Functional and Metabolic Characterization of Microglia Culture in a Defined Medium |
| title_full | Functional and Metabolic Characterization of Microglia Culture in a Defined Medium |
| title_fullStr | Functional and Metabolic Characterization of Microglia Culture in a Defined Medium |
| title_full_unstemmed | Functional and Metabolic Characterization of Microglia Culture in a Defined Medium |
| title_short | Functional and Metabolic Characterization of Microglia Culture in a Defined Medium |
| title_sort | functional and metabolic characterization of microglia culture in a defined medium |
| topic | microglia culture ramification phagocytosis metabolic reprogramming purinergic receptors |
| url | https://www.frontiersin.org/article/10.3389/fncel.2020.00022/full |
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