Wearable cassette for paper-based aptasensor designed to detection of illicit drug; ketamine- a wearable cassette prototype comparison study
The advent of 3D printing technology has spurred innovation, particularly in healthcare and biosensing. One notable application is the creation of wearable biosensors for detecting substances like ketamine, a potent anesthetic and pain reliever with medical and recreational uses. Monitoring ketamine...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Biosensors and Bioelectronics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S259013702400027X |
| _version_ | 1797233998227832832 |
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| author | Shariq Suleman Nigar Anzar Shikha Patil Zaira Azmi Suhel Parvez Jagriti Narang |
| author_facet | Shariq Suleman Nigar Anzar Shikha Patil Zaira Azmi Suhel Parvez Jagriti Narang |
| author_sort | Shariq Suleman |
| collection | DOAJ |
| description | The advent of 3D printing technology has spurred innovation, particularly in healthcare and biosensing. One notable application is the creation of wearable biosensors for detecting substances like ketamine, a potent anesthetic and pain reliever with medical and recreational uses. Monitoring ketamine levels is crucial due to potential misuse and health risks. Utilizing 3D printing, manufacturers can produce intricate and customizable wearable biosensors designed for ketamine detection. This flexibility permits the incorporation of various sensor types, enhancing accuracy. Traditional detection methods are often cumbersome, making 3D printing a transformative tool for real-time monitoring. The application of 3D printing in wearable biosensors has the potential to revolutionize personalized healthcare, ensuring the safe and effective usage of ketamine. In this paper 3D printed paper-based wearable aptamer cassette (3DP-PWC) has been developed by immobilizing Ketamine Aptamer on ZnO-NPs electrodes. Electrochemical techniques such as cyclic voltammetry (CV), linear sweep voltammetry (LSV) and electrochemical impedance spectroscopy (EIS) were employed for validating results. The sensor’s versatility was demonstrated across beverages encompassing both alcoholic and non-alcoholic options. Two prototypes—a bracelet and a pendant—were developed and compared, showing promising results. Here, we reported a 3D-printing paper based wearable aptasensor for the ketamine detection. This pioneering developed sensor showed a low limit detection (LOD) of 0.01 μg/mL (lower than the physiological detection threshold 0.084 μg/mL) with linear-range was between 0.01 and 5 μmL and an optimal response time of 25 s. |
| first_indexed | 2024-04-24T16:25:04Z |
| format | Article |
| id | doaj.art-cb31432f96a4432fa3275ba0c638392e |
| institution | Directory Open Access Journal |
| issn | 2590-1370 |
| language | English |
| last_indexed | 2024-04-24T16:25:04Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj.art-cb31432f96a4432fa3275ba0c638392e2024-03-31T04:37:56ZengElsevierBiosensors and Bioelectronics: X2590-13702024-06-0118100463Wearable cassette for paper-based aptasensor designed to detection of illicit drug; ketamine- a wearable cassette prototype comparison studyShariq Suleman0Nigar Anzar1Shikha Patil2Zaira Azmi3Suhel Parvez4Jagriti Narang5Department of Biotechnology, School of Chemical and Life Science, Jamia Hamdard, New Delhi, 110062, IndiaDepartment of Biotechnology, School of Chemical and Life Science, Jamia Hamdard, New Delhi, 110062, IndiaDepartment of Biotechnology, School of Chemical and Life Science, Jamia Hamdard, New Delhi, 110062, IndiaDepartment of Biotechnology, School of Chemical and Life Science, Jamia Hamdard, New Delhi, 110062, IndiaDepartment of Toxicology, School of Chemical and Life Science, Jamia Hamdard, New Delhi, 110062, IndiaDepartment of Biotechnology, School of Chemical and Life Science, Jamia Hamdard, New Delhi, 110062, India; Corresponding author. Department of Biotechnology, School of Chemical and Life Science, Jamia Hamdard, New Delhi, India.The advent of 3D printing technology has spurred innovation, particularly in healthcare and biosensing. One notable application is the creation of wearable biosensors for detecting substances like ketamine, a potent anesthetic and pain reliever with medical and recreational uses. Monitoring ketamine levels is crucial due to potential misuse and health risks. Utilizing 3D printing, manufacturers can produce intricate and customizable wearable biosensors designed for ketamine detection. This flexibility permits the incorporation of various sensor types, enhancing accuracy. Traditional detection methods are often cumbersome, making 3D printing a transformative tool for real-time monitoring. The application of 3D printing in wearable biosensors has the potential to revolutionize personalized healthcare, ensuring the safe and effective usage of ketamine. In this paper 3D printed paper-based wearable aptamer cassette (3DP-PWC) has been developed by immobilizing Ketamine Aptamer on ZnO-NPs electrodes. Electrochemical techniques such as cyclic voltammetry (CV), linear sweep voltammetry (LSV) and electrochemical impedance spectroscopy (EIS) were employed for validating results. The sensor’s versatility was demonstrated across beverages encompassing both alcoholic and non-alcoholic options. Two prototypes—a bracelet and a pendant—were developed and compared, showing promising results. Here, we reported a 3D-printing paper based wearable aptasensor for the ketamine detection. This pioneering developed sensor showed a low limit detection (LOD) of 0.01 μg/mL (lower than the physiological detection threshold 0.084 μg/mL) with linear-range was between 0.01 and 5 μmL and an optimal response time of 25 s.http://www.sciencedirect.com/science/article/pii/S259013702400027XPaper based electrode3D printed wearable cassetteKetamine drugNanoparticlesAptasensor |
| spellingShingle | Shariq Suleman Nigar Anzar Shikha Patil Zaira Azmi Suhel Parvez Jagriti Narang Wearable cassette for paper-based aptasensor designed to detection of illicit drug; ketamine- a wearable cassette prototype comparison study Biosensors and Bioelectronics: X Paper based electrode 3D printed wearable cassette Ketamine drug Nanoparticles Aptasensor |
| title | Wearable cassette for paper-based aptasensor designed to detection of illicit drug; ketamine- a wearable cassette prototype comparison study |
| title_full | Wearable cassette for paper-based aptasensor designed to detection of illicit drug; ketamine- a wearable cassette prototype comparison study |
| title_fullStr | Wearable cassette for paper-based aptasensor designed to detection of illicit drug; ketamine- a wearable cassette prototype comparison study |
| title_full_unstemmed | Wearable cassette for paper-based aptasensor designed to detection of illicit drug; ketamine- a wearable cassette prototype comparison study |
| title_short | Wearable cassette for paper-based aptasensor designed to detection of illicit drug; ketamine- a wearable cassette prototype comparison study |
| title_sort | wearable cassette for paper based aptasensor designed to detection of illicit drug ketamine a wearable cassette prototype comparison study |
| topic | Paper based electrode 3D printed wearable cassette Ketamine drug Nanoparticles Aptasensor |
| url | http://www.sciencedirect.com/science/article/pii/S259013702400027X |
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