Heating-mediated purification of active FGF21 and structure-based design of its variant with enhanced potency

Heating-mediated purification of active FGF21 and structure-based design of its variant with enhanced potency

Abstract Fibroblast growth factor 21 (FGF21) has pharmaceutical potential against obesity-related metabolic disorders, including non-alcoholic fatty liver disease. Since thermal stability is a desirable factor for therapeutic proteins, we investigated the thermal behavior of human FGF21. FGF21 remai...

Full description

Bibliographic Details
Main Authors:	Ye-Eun Jung, Kyeong Won Lee, Jae Hyun Cho, Da-Woon Bae, Bo-Gyeong Jeong, Yeon-Ju Jung, Soo-Bong Park, Young Jun An, Kyungchan Kim, Ga Seul Lee, Lin-Woo Kang, Jeong Hee Moon, Jung-Hyun Lee, Eun-Kyoung Kim, Hyung-Soon Yim, Sun-Shin Cha
Format:	Article
Language:	English
Published:	Nature Portfolio 2023-01-01
Series:	Scientific Reports
Online Access:	https://doi.org/10.1038/s41598-023-27717-x

Similar Items

FGF11 influences 3T3‐L1 preadipocyte differentiation by modulating the expression of PPARγ regulators
by: Kyeong Won Lee, et al.
Published: (2019-04-01)

Silencing of hypothalamic FGF11 prevents diet-induced obesity
by: Jae Hyun Cho, et al.
Published: (2022-09-01)

Eclipta prostrata promotes the induction of anagen, sustains the anagen phase through regulation of FGF-7 and FGF-5
by: Keun-Hyeun Lee, et al.
Published: (2019-01-01)

Crystal structure of AmpC BER and molecular docking lead to the discovery of broad inhibition activities of halisulfates against β-lactamases
by: Bo-Gyeong Jeong, et al.
Published: (2021-01-01)

Paeonol oxime inhibits bFGF-induced angiogenesis and reduces VEGF levels in fibrosarcoma cells.
by: Hyo-Jeong Lee, et al.
Published: (2010-01-01)

Highly Pathogenic Avian Influenza A(H5N6) in Domestic Cats, South Korea
by: KyungHyun Lee, et al.
Published: (2018-12-01)

Psycho-oncology in Korea: past, present and future
by: Hyun Jeong Lee, et al.
Published: (2017-05-01)

Additive effect of bFGF and selenium on expansion and paracrine action of human amniotic fluid-derived mesenchymal stem cells
by: Junghyun Park, et al.
Published: (2018-11-01)

Injury induces endothelial to mesenchymal transition in the mouse corneal endothelium in vivo via FGF2
by: JeongGoo Lee, et al.
Published: (2019-01-01)

Chrysin-Induced G Protein-Coupled Estrogen Receptor Activation Suppresses Pancreatic Cancer
by: Hyun Kyung Lim, et al.
Published: (2022-08-01)

How to Build a Learning Capability for Innovation? A Framework of Market-Based Learning Process
by: Hyun Jung Lee, et al.
Published: (2015-04-01)

Cytoskeletal alteration modulates cancer cell invasion through RhoA-YAP signaling in stromal fibroblasts.
by: Do Kyeong Kim, et al.
Published: (2019-01-01)

Identification of differentially expressed microRNAs as potential biomarkers for carcinoma ex pleomorphic adenoma
by: Hyojin Kim, et al.
Published: (2022-08-01)

Ultrasonography-guided reduction of pediatric radial neck fractures
by: Jung Eun Lee, et al.
Published: (2017-12-01)

A study on the mechanically equivalent surrogate plate of UMo dispersion fuel using tungsten
by: Hyun-Jung Kim, et al.
Published: (2019-04-01)

Fabrication and Characterization of Al<sub>2</sub>O<sub>3</sub>-Siloxane Composite Thermal Pads for Thermal Interface Materials
by: Seul-Ki Kim, et al.
Published: (2024-02-01)

Complete plastid genome sequence of Neolitsea aciculata (Laurales: Lauraceae), an evergreen broad-leaved tree endemic to East Asia
by: Eun-Kyeong Han, et al.
Published: (2021-09-01)

The Potency of Abamectin Formulations against the Pine Wood Nematode, Bursaphelenchus xylophilus
by: Jong-won Lee, et al.
Published: (2023-06-01)

The complete chloroplast genome sequence of the North American sclerophyllous evergreen shrub, Quercus turbinella (Fagaceae)
by: Amarsanaa Gantsetseg, et al.
Published: (2024-01-01)

The impact of Renin-Angiotensin System Inhibitors on bone fracture risk: a nationwide nested case-control study
by: Kwang Min Kim, et al.
Published: (2024-01-01)

Current evidence for the roles of early-life endocrine disruptors on pubertal timing in girls
by: Jeong Eun Lee, et al.
Published: (2019-06-01)

Optimizing Nitrogen Balance Is Associated with Better Outcomes in Neurocritically Ill Patients
by: Tae Jung Kim, et al.
Published: (2020-10-01)

Characteristics of the Discoloration Switching Phenomenon of 4H-SiC Single Crystals Grown by PVT Method Using ToF-SIMS and Micro-Raman Analysis
by: Seul-Ki Kim, et al.
Published: (2024-02-01)

Association of Tumor Necrosis Factor Inhibitors with the Risk of Nontuberculous Mycobacterial Infection in Patients with Rheumatoid Arthritis: A Nationwide Cohort Study
by: Hyun Jin Park, et al.
Published: (2023-11-01)

Mesoporous Silica Particle as an RNA Adsorbent for Facile Purification of In Vitro-Transcribed RNA
by: Eunbin Cho, et al.
Published: (2023-08-01)

An Immunological Perspective on the Mechanism of Drug Induced Liver Injury: Focused on Drugs for Treatment of Hepatocellular Carcinoma and Liver Transplantation
by: Soon Kyu Lee, et al.
Published: (2023-03-01)

Genetic Architectures and Cell-of-Origin in Glioblastoma
by: Hyun Jung Kim, et al.
Published: (2021-01-01)

Prenatally diagnosed congenital agenesis of the gallbladder: A case report
by: Jeong-suk Kim, et al.
Published: (2022-09-01)

Prognostic Impact of Depends on Combined Tumor Location and Microsatellite Instability Status in Stage II/III Colorectal Cancers Treated with Adjuvant Chemotherapy
by: Hyeon Jeong Oh, et al.
Published: (2019-01-01)

Data resource profile: the Korean Community Health Status Indicators (K-CHSI) database
by: Hye-Eun Lee, et al.
Published: (2023-02-01)

Fractional Carbon Dioxide Laser as an "Add-on" Treatment for Vitiligo: A Meta-analysis with Systematic Review.
by: Hyun Jung Kim, et al.
Published: (2017-11-01)

Diagnostic Strategy of CT Urography as a Prior Examination in the Detection of Bladder Cancer
by: Yeo Kyoung Nam, et al.
Published: (2018-07-01)

Knowledge and Visual Differentiation Ability of the Pressure Injury Classification System and Incontinence-Associated Dermatitis among Hospital Nurses: A Descriptive Study
by: Seungmi Park, et al.
Published: (2024-01-01)

Cytotoxic 4-Hydroxyprorocentrolide and Prorocentrolide C from Cultured Dinoflagellate <i>Prorocentrum lima</i> Induce Human Cancer Cell Death through Apoptosis and Cell Cycle Arrest
by: Seon Min Lee, et al.
Published: (2020-05-01)

Choroidal detachment and hypotony following selective laser trabeculoplasty: a case report
by: Woong Hee Kim, et al.
Published: (2023-06-01)

Comparison of Six Serological Immunoassays for the Detection of SARS-CoV-2 Neutralizing Antibody Levels in the Vaccinated Population
by: Hee-Jung Lee, et al.
Published: (2022-04-01)

Increased Physical Activity Decreases Prevalence of Periodontitis: The Korean National Health and Nutrition Examination Survey (KNHANES Ⅵ) (2013-2015)
by: Eun-Jeong KIM, et al.
Published: (2020-02-01)

Mechanistic Investigation of WWOX Function in NF-kB-Induced Skin Inflammation in Psoriasis
by: Min-Jeong Shin, et al.
Published: (2023-12-01)

Epicardial adipose tissue thickness is an indicator for coronary artery stenosis in asymptomatic type 2 diabetic patients: its assessment by cardiac magnetic resonance
by: Kim Hyun, et al.
Published: (2012-07-01)

Immune-Enhancing Effects of Red <i>Platycodon grandiflorus</i> Root Extract via p38 MAPK-Mediated NF-κB Activation
by: Eun-Jung Park, et al.
Published: (2020-08-01)