Perlite as a Biocarrier for Augmentation of Biogas-Producing Reactors from Olive (<i>Olea europaea</i>) Waste
Biogas is mainly produced by anaerobic digestion (AD), and in the EU, the widely used substrate for AD is maize silage. Due to a rise in silage prices, the intention is to gradually replace maize with lignocellulose biomass. In the Mediterranean area, the olive industry produces large amounts of lig...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/17/8808 |
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| author | Tomislav Ivankovic Mislav Kontek Valentino Mihalic Antonia Ressler Vanja Jurisic |
| author_facet | Tomislav Ivankovic Mislav Kontek Valentino Mihalic Antonia Ressler Vanja Jurisic |
| author_sort | Tomislav Ivankovic |
| collection | DOAJ |
| description | Biogas is mainly produced by anaerobic digestion (AD), and in the EU, the widely used substrate for AD is maize silage. Due to a rise in silage prices, the intention is to gradually replace maize with lignocellulose biomass. In the Mediterranean area, the olive industry produces large amounts of lignocellulose wastes, namely olive cake and pruned biomass. Still, due to its high lignin content, it is resistant to biodegradation. This issue could be resolved by adding targeted microorganisms that enhance the substrate’s primary degradation, and the cells’ attachment to suitable biocarriers could boost the augmentation process. A microbial consortium customized for biodegradation of olive cake and pruned biomass was isolated, propagated and immobilized onto the biocarrier, perlite, a naturally occurring aluminosilicate material. The perlite proved to be a suitable biocarrier with numbers of immobilized bacteria as high as 2.1 ± 0.9 × 10<sup>11</sup> and 3.4 ± 0.6 × 10<sup>10</sup> CFU g<sup>−1</sup> when preparation was performed in aerobic and anaerobic conditions, respectively. Bioaugmentation of AD reactors significantly increased the biogas yield, but only if olive cake, not the pruned biomass, was used as a substrate. |
| first_indexed | 2024-03-10T03:01:10Z |
| format | Article |
| id | doaj.art-cb3b2de4d86e4426a7408f28b29fdb35 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T03:01:10Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-cb3b2de4d86e4426a7408f28b29fdb352023-11-23T12:47:15ZengMDPI AGApplied Sciences2076-34172022-09-011217880810.3390/app12178808Perlite as a Biocarrier for Augmentation of Biogas-Producing Reactors from Olive (<i>Olea europaea</i>) WasteTomislav Ivankovic0Mislav Kontek1Valentino Mihalic2Antonia Ressler3Vanja Jurisic4Faculty of Science, Department of Biology, University of Zagreb, 10000 Zagreb, CroatiaFaculty of Agriculture, University of Zagreb, 10000 Zagreb, CroatiaFaculty of Science, Department of Biology, University of Zagreb, 10000 Zagreb, CroatiaFaculty of Chemical Engineering and Technology, University of Zagreb, 10000 Zagreb, CroatiaFaculty of Agriculture, University of Zagreb, 10000 Zagreb, CroatiaBiogas is mainly produced by anaerobic digestion (AD), and in the EU, the widely used substrate for AD is maize silage. Due to a rise in silage prices, the intention is to gradually replace maize with lignocellulose biomass. In the Mediterranean area, the olive industry produces large amounts of lignocellulose wastes, namely olive cake and pruned biomass. Still, due to its high lignin content, it is resistant to biodegradation. This issue could be resolved by adding targeted microorganisms that enhance the substrate’s primary degradation, and the cells’ attachment to suitable biocarriers could boost the augmentation process. A microbial consortium customized for biodegradation of olive cake and pruned biomass was isolated, propagated and immobilized onto the biocarrier, perlite, a naturally occurring aluminosilicate material. The perlite proved to be a suitable biocarrier with numbers of immobilized bacteria as high as 2.1 ± 0.9 × 10<sup>11</sup> and 3.4 ± 0.6 × 10<sup>10</sup> CFU g<sup>−1</sup> when preparation was performed in aerobic and anaerobic conditions, respectively. Bioaugmentation of AD reactors significantly increased the biogas yield, but only if olive cake, not the pruned biomass, was used as a substrate.https://www.mdpi.com/2076-3417/12/17/8808bioaugmentationbiogas productionolive wastepruned biomassolive cakebiogas substrate |
| spellingShingle | Tomislav Ivankovic Mislav Kontek Valentino Mihalic Antonia Ressler Vanja Jurisic Perlite as a Biocarrier for Augmentation of Biogas-Producing Reactors from Olive (<i>Olea europaea</i>) Waste Applied Sciences bioaugmentation biogas production olive waste pruned biomass olive cake biogas substrate |
| title | Perlite as a Biocarrier for Augmentation of Biogas-Producing Reactors from Olive (<i>Olea europaea</i>) Waste |
| title_full | Perlite as a Biocarrier for Augmentation of Biogas-Producing Reactors from Olive (<i>Olea europaea</i>) Waste |
| title_fullStr | Perlite as a Biocarrier for Augmentation of Biogas-Producing Reactors from Olive (<i>Olea europaea</i>) Waste |
| title_full_unstemmed | Perlite as a Biocarrier for Augmentation of Biogas-Producing Reactors from Olive (<i>Olea europaea</i>) Waste |
| title_short | Perlite as a Biocarrier for Augmentation of Biogas-Producing Reactors from Olive (<i>Olea europaea</i>) Waste |
| title_sort | perlite as a biocarrier for augmentation of biogas producing reactors from olive i olea europaea i waste |
| topic | bioaugmentation biogas production olive waste pruned biomass olive cake biogas substrate |
| url | https://www.mdpi.com/2076-3417/12/17/8808 |
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