Effect of Caging on <i>Cryptosporidium parvum</i> Proliferation in Mice
Cryptosporidiosis is an enteric infection caused by several protozoan species in the genus <i>Cryptosporidium</i> (phylum Apicomplexa). Immunosuppressed mice are commonly used to model this infection. Surprisingly, for a pathogen like <i>Cryptosporidium parvum</i>, which is r...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/6/1242 |
| _version_ | 1797484226337046528 |
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| author | Hannah N. Creasey Wen Zhang Giovanni Widmer |
| author_facet | Hannah N. Creasey Wen Zhang Giovanni Widmer |
| author_sort | Hannah N. Creasey |
| collection | DOAJ |
| description | Cryptosporidiosis is an enteric infection caused by several protozoan species in the genus <i>Cryptosporidium</i> (phylum Apicomplexa). Immunosuppressed mice are commonly used to model this infection. Surprisingly, for a pathogen like <i>Cryptosporidium parvum</i>, which is readily transmitted fecal-orally, mice housed in the same cage can develop vastly different levels of infection, ranging from undetectable to lethal. The motivation for this study was to investigate this phenomenon and assess the association between the severity of cryptosporidiosis and the fecal microbiota. To this aim, the association between severity of cryptosporidiosis and caging (group caged vs. individually caged) and between the microbiota taxonomy and the course of the infection was examined. In contrast to mice caged in groups of four, a majority of mice caged individually did not excrete a detectable level of oocysts. Microbiota α diversity in samples collected between three days prior to infection and one day post-infection was negatively correlated with the severity of cryptosporidiosis, suggesting a causal negative relationship between microbiota diversity and susceptibility to <i>C. parvum</i>. |
| first_indexed | 2024-03-09T22:59:23Z |
| format | Article |
| id | doaj.art-cb3b80f7a72d4e088976967edc0c35dc |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T22:59:23Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-cb3b80f7a72d4e088976967edc0c35dc2023-11-23T18:05:26ZengMDPI AGMicroorganisms2076-26072022-06-01106124210.3390/microorganisms10061242Effect of Caging on <i>Cryptosporidium parvum</i> Proliferation in MiceHannah N. Creasey0Wen Zhang1Giovanni Widmer2Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536, USACummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536, USACummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536, USACryptosporidiosis is an enteric infection caused by several protozoan species in the genus <i>Cryptosporidium</i> (phylum Apicomplexa). Immunosuppressed mice are commonly used to model this infection. Surprisingly, for a pathogen like <i>Cryptosporidium parvum</i>, which is readily transmitted fecal-orally, mice housed in the same cage can develop vastly different levels of infection, ranging from undetectable to lethal. The motivation for this study was to investigate this phenomenon and assess the association between the severity of cryptosporidiosis and the fecal microbiota. To this aim, the association between severity of cryptosporidiosis and caging (group caged vs. individually caged) and between the microbiota taxonomy and the course of the infection was examined. In contrast to mice caged in groups of four, a majority of mice caged individually did not excrete a detectable level of oocysts. Microbiota α diversity in samples collected between three days prior to infection and one day post-infection was negatively correlated with the severity of cryptosporidiosis, suggesting a causal negative relationship between microbiota diversity and susceptibility to <i>C. parvum</i>.https://www.mdpi.com/2076-2607/10/6/1242<i>Cryptosporidium</i>cryptosporidiosismicrobiotaconstrained ordinationdysbiosis |
| spellingShingle | Hannah N. Creasey Wen Zhang Giovanni Widmer Effect of Caging on <i>Cryptosporidium parvum</i> Proliferation in Mice Microorganisms <i>Cryptosporidium</i> cryptosporidiosis microbiota constrained ordination dysbiosis |
| title | Effect of Caging on <i>Cryptosporidium parvum</i> Proliferation in Mice |
| title_full | Effect of Caging on <i>Cryptosporidium parvum</i> Proliferation in Mice |
| title_fullStr | Effect of Caging on <i>Cryptosporidium parvum</i> Proliferation in Mice |
| title_full_unstemmed | Effect of Caging on <i>Cryptosporidium parvum</i> Proliferation in Mice |
| title_short | Effect of Caging on <i>Cryptosporidium parvum</i> Proliferation in Mice |
| title_sort | effect of caging on i cryptosporidium parvum i proliferation in mice |
| topic | <i>Cryptosporidium</i> cryptosporidiosis microbiota constrained ordination dysbiosis |
| url | https://www.mdpi.com/2076-2607/10/6/1242 |
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