Use of Staged Molecular Analysis to Determine Causes of Unexplained Central Nervous System Infections
No agent is implicated in most central nervous system (CNS) infections. To investigate cerebrospinal fluid samples from patients with CNS infections of unknown cause in 1 hospital in Taiwan, we used a staged molecular approach, incorporating techniques including multiplex MassTag PCR, 16S rRNA PCR,...
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2013-09-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/19/9/13-0474_article |
| _version_ | 1818259720315600896 |
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| author | Chien-Chin Hsu Rafal Tokarz Thomas Briese Hung-Chin Tsai Phenix-Lan Quan W. Ian Lipkin |
| author_facet | Chien-Chin Hsu Rafal Tokarz Thomas Briese Hung-Chin Tsai Phenix-Lan Quan W. Ian Lipkin |
| author_sort | Chien-Chin Hsu |
| collection | DOAJ |
| description | No agent is implicated in most central nervous system (CNS) infections. To investigate cerebrospinal fluid samples from patients with CNS infections of unknown cause in 1 hospital in Taiwan, we used a staged molecular approach, incorporating techniques including multiplex MassTag PCR, 16S rRNA PCR, DNA microarray, and high-throughput pyrosequencing. We determined the infectious agent for 31 (24%) of 131 previously negative samples. Candidate pathogens were identified for 25 (27%) of 94 unexplained meningitis cases and 6 (16%) of 37 unexplained encephalitis cases. Epstein-Barr virus (18 infections) accounted for most of the identified agents in unexplained meningitis cases, followed by Escherichia coli (5), enterovirus (2), human herpesvirus 2 (1), and Mycobacterium tuberculosis. Herpesviruses were identified in samples from patients with unexplained encephalitis cases, including varicella-zoster virus (3 infections), human herpesvirus 1 (2), and cytomegalovirus (1). Our study confirms the power of multiplex MassTag PCR as a rapid diagnostic tool for identifying pathogens causing unexplained CNS infections. |
| first_indexed | 2024-12-12T18:19:55Z |
| format | Article |
| id | doaj.art-cb3b95d7590e4a0a8278c04c8b2e84db |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-12T18:19:55Z |
| publishDate | 2013-09-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-cb3b95d7590e4a0a8278c04c8b2e84db2022-12-22T00:16:11ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592013-09-011991470147710.3201/eid1909.130474Use of Staged Molecular Analysis to Determine Causes of Unexplained Central Nervous System InfectionsChien-Chin HsuRafal TokarzThomas BrieseHung-Chin TsaiPhenix-Lan QuanW. Ian LipkinNo agent is implicated in most central nervous system (CNS) infections. To investigate cerebrospinal fluid samples from patients with CNS infections of unknown cause in 1 hospital in Taiwan, we used a staged molecular approach, incorporating techniques including multiplex MassTag PCR, 16S rRNA PCR, DNA microarray, and high-throughput pyrosequencing. We determined the infectious agent for 31 (24%) of 131 previously negative samples. Candidate pathogens were identified for 25 (27%) of 94 unexplained meningitis cases and 6 (16%) of 37 unexplained encephalitis cases. Epstein-Barr virus (18 infections) accounted for most of the identified agents in unexplained meningitis cases, followed by Escherichia coli (5), enterovirus (2), human herpesvirus 2 (1), and Mycobacterium tuberculosis. Herpesviruses were identified in samples from patients with unexplained encephalitis cases, including varicella-zoster virus (3 infections), human herpesvirus 1 (2), and cytomegalovirus (1). Our study confirms the power of multiplex MassTag PCR as a rapid diagnostic tool for identifying pathogens causing unexplained CNS infections.https://wwwnc.cdc.gov/eid/article/19/9/13-0474_articleCNS infectionsmeningitisencephalitisMassTag PCR16S rRNA PCRmicroarray |
| spellingShingle | Chien-Chin Hsu Rafal Tokarz Thomas Briese Hung-Chin Tsai Phenix-Lan Quan W. Ian Lipkin Use of Staged Molecular Analysis to Determine Causes of Unexplained Central Nervous System Infections Emerging Infectious Diseases CNS infections meningitis encephalitis MassTag PCR 16S rRNA PCR microarray |
| title | Use of Staged Molecular Analysis to Determine Causes of Unexplained Central Nervous System Infections |
| title_full | Use of Staged Molecular Analysis to Determine Causes of Unexplained Central Nervous System Infections |
| title_fullStr | Use of Staged Molecular Analysis to Determine Causes of Unexplained Central Nervous System Infections |
| title_full_unstemmed | Use of Staged Molecular Analysis to Determine Causes of Unexplained Central Nervous System Infections |
| title_short | Use of Staged Molecular Analysis to Determine Causes of Unexplained Central Nervous System Infections |
| title_sort | use of staged molecular analysis to determine causes of unexplained central nervous system infections |
| topic | CNS infections meningitis encephalitis MassTag PCR 16S rRNA PCR microarray |
| url | https://wwwnc.cdc.gov/eid/article/19/9/13-0474_article |
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