Isolation and Antimicrobial Activities of Lactic Acid Bacteria Originated From Indonesian Local Goat’s Colostrum
The objectives of this study were to isolate lactic acid bacteria (LAB) from Indonesian local goat colostrum and to characterize their suitable properties for bacteriocin production. LAB was isolated from goat colostrum. The characterization of LAB was carried out based on the shape, colony dispersa...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Universitas Jenderal Soedirman (UNSOED), Faculty of Animal Science
2018-09-01
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| Series: | Animal Production: Indonesian Journal of Animal Production |
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| Online Access: | http://animalproduction.net/index.php/JAP/article/view/731 |
| _version_ | 1797231159467311104 |
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| author | Triana Setyawardani Juni Sumarmono |
| author_facet | Triana Setyawardani Juni Sumarmono |
| author_sort | Triana Setyawardani |
| collection | DOAJ |
| description | The objectives of this study were to isolate lactic acid bacteria (LAB) from Indonesian local goat colostrum and to characterize their suitable properties for bacteriocin production. LAB was isolated from goat colostrum. The characterization of LAB was carried out based on the shape, colony dispersal, and catalase test. For antimicrobial activity, LAB was tested by a well diffusion method followed by an antimicrobial activity test against pathogenic bacteria B. cereus, E.coli, S. aureus and S. thypimurium. A total of 8 strains of LAB were successfully isolated from goat colostrum and coded CT1 to CT8. All the isolates were rod-shaped, single or paired colonies, negative catalase, and glucose fermenting LAB. The isolates consist of four L. casei, two L. brevis or L. plantarum, one L. rhamnosus, and one, L. paracasei. CT3 isolate has 84% similarity with L. plantarum and 14.3% with L. brevis 1while CT8 isolate is 71% similar to L. brevis 1 and 28.9% to L. plantarum. Purity evaluation showed that CT3 and CT8 were L. plantarum. Well difusion test showed that all LAB strains possess very solid resistances, with diameters over 17 mm, against B. cereus, E.coli, S. aureus and S. thypimurium. The average inhibitory resistance against B. Cereus, E.coli, S.aureus and S.typhimurium was was 17.68 mm, 19.38, 19.30 and 19.03 mm, respectively. LAB isolated from Indonesian local goat colostrum are potential candidates for bacteriocin-producing bacteria. |
| first_indexed | 2024-04-24T15:39:57Z |
| format | Article |
| id | doaj.art-cb3c5978098040d4b4f8b506d7c51814 |
| institution | Directory Open Access Journal |
| issn | 2541-5875 2541-5875 |
| language | English |
| last_indexed | 2024-04-24T15:39:57Z |
| publishDate | 2018-09-01 |
| publisher | Universitas Jenderal Soedirman (UNSOED), Faculty of Animal Science |
| record_format | Article |
| series | Animal Production: Indonesian Journal of Animal Production |
| spelling | doaj.art-cb3c5978098040d4b4f8b506d7c518142024-04-02T00:33:56ZengUniversitas Jenderal Soedirman (UNSOED), Faculty of Animal ScienceAnimal Production: Indonesian Journal of Animal Production2541-58752541-58752018-09-0120317318110.20884/1.jap.2018.20.3.731515Isolation and Antimicrobial Activities of Lactic Acid Bacteria Originated From Indonesian Local Goat’s ColostrumTriana Setyawardani0Juni Sumarmono1Faculty of Animal Science, Jenderal Soedirman UniversityFaculty of Animal Science, Jenderal Soedirman UniversityThe objectives of this study were to isolate lactic acid bacteria (LAB) from Indonesian local goat colostrum and to characterize their suitable properties for bacteriocin production. LAB was isolated from goat colostrum. The characterization of LAB was carried out based on the shape, colony dispersal, and catalase test. For antimicrobial activity, LAB was tested by a well diffusion method followed by an antimicrobial activity test against pathogenic bacteria B. cereus, E.coli, S. aureus and S. thypimurium. A total of 8 strains of LAB were successfully isolated from goat colostrum and coded CT1 to CT8. All the isolates were rod-shaped, single or paired colonies, negative catalase, and glucose fermenting LAB. The isolates consist of four L. casei, two L. brevis or L. plantarum, one L. rhamnosus, and one, L. paracasei. CT3 isolate has 84% similarity with L. plantarum and 14.3% with L. brevis 1while CT8 isolate is 71% similar to L. brevis 1 and 28.9% to L. plantarum. Purity evaluation showed that CT3 and CT8 were L. plantarum. Well difusion test showed that all LAB strains possess very solid resistances, with diameters over 17 mm, against B. cereus, E.coli, S. aureus and S. thypimurium. The average inhibitory resistance against B. Cereus, E.coli, S.aureus and S.typhimurium was was 17.68 mm, 19.38, 19.30 and 19.03 mm, respectively. LAB isolated from Indonesian local goat colostrum are potential candidates for bacteriocin-producing bacteria.http://animalproduction.net/index.php/JAP/article/view/731colostrum, lactic acid bacteria, pathogen, antimicrobial activity |
| spellingShingle | Triana Setyawardani Juni Sumarmono Isolation and Antimicrobial Activities of Lactic Acid Bacteria Originated From Indonesian Local Goat’s Colostrum Animal Production: Indonesian Journal of Animal Production colostrum, lactic acid bacteria, pathogen, antimicrobial activity |
| title | Isolation and Antimicrobial Activities of Lactic Acid Bacteria Originated From Indonesian Local Goat’s Colostrum |
| title_full | Isolation and Antimicrobial Activities of Lactic Acid Bacteria Originated From Indonesian Local Goat’s Colostrum |
| title_fullStr | Isolation and Antimicrobial Activities of Lactic Acid Bacteria Originated From Indonesian Local Goat’s Colostrum |
| title_full_unstemmed | Isolation and Antimicrobial Activities of Lactic Acid Bacteria Originated From Indonesian Local Goat’s Colostrum |
| title_short | Isolation and Antimicrobial Activities of Lactic Acid Bacteria Originated From Indonesian Local Goat’s Colostrum |
| title_sort | isolation and antimicrobial activities of lactic acid bacteria originated from indonesian local goat s colostrum |
| topic | colostrum, lactic acid bacteria, pathogen, antimicrobial activity |
| url | http://animalproduction.net/index.php/JAP/article/view/731 |
| work_keys_str_mv | AT trianasetyawardani isolationandantimicrobialactivitiesoflacticacidbacteriaoriginatedfromindonesianlocalgoatscolostrum AT junisumarmono isolationandantimicrobialactivitiesoflacticacidbacteriaoriginatedfromindonesianlocalgoatscolostrum |