Novel Inactivated Subtype B Avian Metapneumovirus Vaccine Induced Humoral and Cellular Immune Responses
Avian metapneumovirus (aMPV), a highly contagious agent, is widespread and causes acute upper respiratory tract disease in chickens and turkeys. However, currently, there is no vaccine licensed in China. Herein, we describe the development of an inactivated aMPV/B vaccine using the aMPV/B strain LN1...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/8/4/762 |
| _version_ | 1797544778925080576 |
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| author | Yuanling Bao Mengmeng Yu Peng Liu Fujun Hou Farooque Muhammad Zhihao Wang Xinyi Li Zhuo Zhang Suyan Wang Yuntong Chen Hongyu Cui Aijing Liu Xiaole Qi Qing Pan Yanping Zhang Li Gao Kai Li Changjun Liu Xijun He Xiaomei Wang Yulong Gao |
| author_facet | Yuanling Bao Mengmeng Yu Peng Liu Fujun Hou Farooque Muhammad Zhihao Wang Xinyi Li Zhuo Zhang Suyan Wang Yuntong Chen Hongyu Cui Aijing Liu Xiaole Qi Qing Pan Yanping Zhang Li Gao Kai Li Changjun Liu Xijun He Xiaomei Wang Yulong Gao |
| author_sort | Yuanling Bao |
| collection | DOAJ |
| description | Avian metapneumovirus (aMPV), a highly contagious agent, is widespread and causes acute upper respiratory tract disease in chickens and turkeys. However, currently, there is no vaccine licensed in China. Herein, we describe the development of an inactivated aMPV/B vaccine using the aMPV/B strain LN16. Combined with a novel adjuvant containing immune-stimulating complexes (ISCOMs), the novel vaccine could induce high virus-specific and VN antibodies. In addition, it activated B and T lymphocytes and promoted the expression of IL-4 and IFN-γ. Importantly, boosting vaccination with the inactivated aMPV/B vaccine could provide 100% protection against aMPV/B infection with reduced virus shedding and turbinate inflammation. The protection efficacy could last for at least 6 months. This study yielded a novel inactivated aMPV/B vaccine that could serve as the first vaccine candidate in China, thus contributing to the control of aMPV/B and promoting the development of the poultry industry. |
| first_indexed | 2024-03-10T14:05:17Z |
| format | Article |
| id | doaj.art-cb3ceeb6da224d84996148b5d4d7ec68 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T14:05:17Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-cb3ceeb6da224d84996148b5d4d7ec682023-11-21T00:44:42ZengMDPI AGVaccines2076-393X2020-12-018476210.3390/vaccines8040762Novel Inactivated Subtype B Avian Metapneumovirus Vaccine Induced Humoral and Cellular Immune ResponsesYuanling Bao0Mengmeng Yu1Peng Liu2Fujun Hou3Farooque Muhammad4Zhihao Wang5Xinyi Li6Zhuo Zhang7Suyan Wang8Yuntong Chen9Hongyu Cui10Aijing Liu11Xiaole Qi12Qing Pan13Yanping Zhang14Li Gao15Kai Li16Changjun Liu17Xijun He18Xiaomei Wang19Yulong Gao20State Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaAohan County Breeding and Extension Center, Chifeng, Inner Mongolia 024300, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Avian Immunosuppressive Diseases Division, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaAvian metapneumovirus (aMPV), a highly contagious agent, is widespread and causes acute upper respiratory tract disease in chickens and turkeys. However, currently, there is no vaccine licensed in China. Herein, we describe the development of an inactivated aMPV/B vaccine using the aMPV/B strain LN16. Combined with a novel adjuvant containing immune-stimulating complexes (ISCOMs), the novel vaccine could induce high virus-specific and VN antibodies. In addition, it activated B and T lymphocytes and promoted the expression of IL-4 and IFN-γ. Importantly, boosting vaccination with the inactivated aMPV/B vaccine could provide 100% protection against aMPV/B infection with reduced virus shedding and turbinate inflammation. The protection efficacy could last for at least 6 months. This study yielded a novel inactivated aMPV/B vaccine that could serve as the first vaccine candidate in China, thus contributing to the control of aMPV/B and promoting the development of the poultry industry.https://www.mdpi.com/2076-393X/8/4/762avian metapneumovirusinactivated vaccineadjuvant |
| spellingShingle | Yuanling Bao Mengmeng Yu Peng Liu Fujun Hou Farooque Muhammad Zhihao Wang Xinyi Li Zhuo Zhang Suyan Wang Yuntong Chen Hongyu Cui Aijing Liu Xiaole Qi Qing Pan Yanping Zhang Li Gao Kai Li Changjun Liu Xijun He Xiaomei Wang Yulong Gao Novel Inactivated Subtype B Avian Metapneumovirus Vaccine Induced Humoral and Cellular Immune Responses Vaccines avian metapneumovirus inactivated vaccine adjuvant |
| title | Novel Inactivated Subtype B Avian Metapneumovirus Vaccine Induced Humoral and Cellular Immune Responses |
| title_full | Novel Inactivated Subtype B Avian Metapneumovirus Vaccine Induced Humoral and Cellular Immune Responses |
| title_fullStr | Novel Inactivated Subtype B Avian Metapneumovirus Vaccine Induced Humoral and Cellular Immune Responses |
| title_full_unstemmed | Novel Inactivated Subtype B Avian Metapneumovirus Vaccine Induced Humoral and Cellular Immune Responses |
| title_short | Novel Inactivated Subtype B Avian Metapneumovirus Vaccine Induced Humoral and Cellular Immune Responses |
| title_sort | novel inactivated subtype b avian metapneumovirus vaccine induced humoral and cellular immune responses |
| topic | avian metapneumovirus inactivated vaccine adjuvant |
| url | https://www.mdpi.com/2076-393X/8/4/762 |
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