Combining Culture-Dependent and Independent Approaches for the Optimization of Epoxiconazole and Fludioxonil-Degrading Bacterial Consortia
Epoxiconazole (EPO) and fludioxonil (FLU) are two widely used fluorinated pesticides known to be highly persistent and with high ecotoxicological potential, turning them into pollutants of concern. This work aimed to optimize two degrading bacterial consortia, previously obtained from an agricultura...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/10/2109 |
| _version_ | 1797513787189755904 |
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| author | Diogo A. M. Alexandrino Ana P. Mucha Maria Paola Tomasino C. Marisa R. Almeida Maria F. Carvalho |
| author_facet | Diogo A. M. Alexandrino Ana P. Mucha Maria Paola Tomasino C. Marisa R. Almeida Maria F. Carvalho |
| author_sort | Diogo A. M. Alexandrino |
| collection | DOAJ |
| description | Epoxiconazole (EPO) and fludioxonil (FLU) are two widely used fluorinated pesticides known to be highly persistent and with high ecotoxicological potential, turning them into pollutants of concern. This work aimed to optimize two degrading bacterial consortia, previously obtained from an agricultural soil through enrichment with EPO and FLU, by characterizing the contribution of their corresponding bacterial isolates to the biodegradation of these pesticides using both culture-dependent and independent methodologies. Results showed that a co-culture of the strains <i>Hydrogenophaga eletricum</i> 5AE and <i>Methylobacillus</i> sp. 8AE was the most efficient in biodegrading EPO, being able to defluorinate ca. 80% of this pesticide in 28 days. This catabolic performance is likely the result of a commensalistic cooperation, in which <i>H. eletricum</i> may be the defluorinating strain and <i>Methylobacillus</i> sp. may assume an accessory, yet pivotal, catabolic role. Furthermore, 16S rRNA metabarcoding analysis revealed that these strains represent a minority in their original consortium, showing that the biodegradation of EPO can be driven by less abundant phylotypes in the community. On the other hand, none of the tested combinations of bacterial strains showed potential to biodegrade FLU, indicating that the key degrading strains were not successfully isolated from the original enrichment culture. Overall, this work shows, for the first time, the direct involvement of two bacterial species, namely <i>H. eletricum</i> and <i>Methylobacillus</i> sp., in the biodegradation of EPO, while also offering insight on how they might cooperate to accomplish this process. Moreover, the importance of adequate culture-dependent approaches in the engineering of microbial consortia for bioremediation purposes is also emphasized. |
| first_indexed | 2024-03-10T06:22:26Z |
| format | Article |
| id | doaj.art-cb3d411dcad64cfcbe40e260d5bc46ef |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T06:22:26Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-cb3d411dcad64cfcbe40e260d5bc46ef2023-11-22T19:14:26ZengMDPI AGMicroorganisms2076-26072021-10-01910210910.3390/microorganisms9102109Combining Culture-Dependent and Independent Approaches for the Optimization of Epoxiconazole and Fludioxonil-Degrading Bacterial ConsortiaDiogo A. M. Alexandrino0Ana P. Mucha1Maria Paola Tomasino2C. Marisa R. Almeida3Maria F. Carvalho4CIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos s/n, 4450-208 Matosinhos, PortugalCIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos s/n, 4450-208 Matosinhos, PortugalCIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos s/n, 4450-208 Matosinhos, PortugalCIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos s/n, 4450-208 Matosinhos, PortugalCIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos s/n, 4450-208 Matosinhos, PortugalEpoxiconazole (EPO) and fludioxonil (FLU) are two widely used fluorinated pesticides known to be highly persistent and with high ecotoxicological potential, turning them into pollutants of concern. This work aimed to optimize two degrading bacterial consortia, previously obtained from an agricultural soil through enrichment with EPO and FLU, by characterizing the contribution of their corresponding bacterial isolates to the biodegradation of these pesticides using both culture-dependent and independent methodologies. Results showed that a co-culture of the strains <i>Hydrogenophaga eletricum</i> 5AE and <i>Methylobacillus</i> sp. 8AE was the most efficient in biodegrading EPO, being able to defluorinate ca. 80% of this pesticide in 28 days. This catabolic performance is likely the result of a commensalistic cooperation, in which <i>H. eletricum</i> may be the defluorinating strain and <i>Methylobacillus</i> sp. may assume an accessory, yet pivotal, catabolic role. Furthermore, 16S rRNA metabarcoding analysis revealed that these strains represent a minority in their original consortium, showing that the biodegradation of EPO can be driven by less abundant phylotypes in the community. On the other hand, none of the tested combinations of bacterial strains showed potential to biodegrade FLU, indicating that the key degrading strains were not successfully isolated from the original enrichment culture. Overall, this work shows, for the first time, the direct involvement of two bacterial species, namely <i>H. eletricum</i> and <i>Methylobacillus</i> sp., in the biodegradation of EPO, while also offering insight on how they might cooperate to accomplish this process. Moreover, the importance of adequate culture-dependent approaches in the engineering of microbial consortia for bioremediation purposes is also emphasized.https://www.mdpi.com/2076-2607/9/10/2109culture-dependent approachesdefluorinationepoxiconazolefludioxonilpersistent pesticidesmetabarcoding |
| spellingShingle | Diogo A. M. Alexandrino Ana P. Mucha Maria Paola Tomasino C. Marisa R. Almeida Maria F. Carvalho Combining Culture-Dependent and Independent Approaches for the Optimization of Epoxiconazole and Fludioxonil-Degrading Bacterial Consortia Microorganisms culture-dependent approaches defluorination epoxiconazole fludioxonil persistent pesticides metabarcoding |
| title | Combining Culture-Dependent and Independent Approaches for the Optimization of Epoxiconazole and Fludioxonil-Degrading Bacterial Consortia |
| title_full | Combining Culture-Dependent and Independent Approaches for the Optimization of Epoxiconazole and Fludioxonil-Degrading Bacterial Consortia |
| title_fullStr | Combining Culture-Dependent and Independent Approaches for the Optimization of Epoxiconazole and Fludioxonil-Degrading Bacterial Consortia |
| title_full_unstemmed | Combining Culture-Dependent and Independent Approaches for the Optimization of Epoxiconazole and Fludioxonil-Degrading Bacterial Consortia |
| title_short | Combining Culture-Dependent and Independent Approaches for the Optimization of Epoxiconazole and Fludioxonil-Degrading Bacterial Consortia |
| title_sort | combining culture dependent and independent approaches for the optimization of epoxiconazole and fludioxonil degrading bacterial consortia |
| topic | culture-dependent approaches defluorination epoxiconazole fludioxonil persistent pesticides metabarcoding |
| url | https://www.mdpi.com/2076-2607/9/10/2109 |
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