Red-Osier Dogwood Extracts Prevent Inflammatory Responses in Caco-2 Cells and a Caco-2 BBe1/EA.hy926 Cell Co-Culture Model
Red-osier dogwood extracts (RDE) contain high levels of phenolic compounds which have been recognized as natural antioxidants. In this study, the potential of RDE to prevent cardiovascular diseases (CVDs) was evaluated using Caco-2 cells and a co-culture model of Caco-2 BBe1/EA.hy926 cells in Transw...
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| Format: | Article |
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MDPI AG
2019-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/8/10/428 |
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| author | Qian Jiang Hua Zhang Runqiang Yang Qianru Hui Yuhuan Chen Lili Mats Rong Tsao Chengbo Yang |
| author_facet | Qian Jiang Hua Zhang Runqiang Yang Qianru Hui Yuhuan Chen Lili Mats Rong Tsao Chengbo Yang |
| author_sort | Qian Jiang |
| collection | DOAJ |
| description | Red-osier dogwood extracts (RDE) contain high levels of phenolic compounds which have been recognized as natural antioxidants. In this study, the potential of RDE to prevent cardiovascular diseases (CVDs) was evaluated using Caco-2 cells and a co-culture model of Caco-2 BBe1/EA.hy926 cells in Transwell<sup>®</sup> plates. The results showed that RDE supplementation significantly prevented interleukin-8 (IL-8) production and suppressed the gene expression of IL-8, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and cyclooxygenase 2 (COX-2) in the TNF-α inflamed Caco-2 cells. Meanwhile, the polyphenols (quercetin-3-glucoside, quercetin-glucuronide, rutin, quercetin-3-<i>O</i>-malonylglucoside, and kaempferol-glucoside) in the RDE were validated to be absorbed by Caco-2 BBe1 cells and transported to the basal chamber where EA.hy926 cells were located during 12 h incubation. The transported polyphenols were able to prevent IL-8 production and suppress the gene expression of proinflammatory mediators (TNF-α, ICAM-1, VCAM-1, and COX-2) in the TNF-α or oxidized low-density lipoprotein (ox-LDL) treated EA.hy926 cells. These novel findings demonstrated that phenolic compounds in RDE can be transported to the cardiovascular system by intestinal absorption and mitigate the inflammatory responses of vascular endothelial cells, indicating that RDE could be a natural resource of polyphenols to prevent inflammation cytokine or oxidized lipid-induced CVDs. |
| first_indexed | 2024-03-12T18:27:26Z |
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| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T18:27:26Z |
| publishDate | 2019-09-01 |
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| series | Antioxidants |
| spelling | doaj.art-cb3da576fc4c49ee8a543e7bde5dd7672023-08-02T08:29:39ZengMDPI AGAntioxidants2076-39212019-09-0181042810.3390/antiox8100428antiox8100428Red-Osier Dogwood Extracts Prevent Inflammatory Responses in Caco-2 Cells and a Caco-2 BBe1/EA.hy926 Cell Co-Culture ModelQian Jiang0Hua Zhang1Runqiang Yang2Qianru Hui3Yuhuan Chen4Lili Mats5Rong Tsao6Chengbo Yang7Department of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaFood Nutrition and Safety, Department of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, ChinaDepartment of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaDepartment of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaGuelph Research & Development Centre, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, CanadaGuelph Research & Development Centre, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, CanadaGuelph Research & Development Centre, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, CanadaDepartment of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaRed-osier dogwood extracts (RDE) contain high levels of phenolic compounds which have been recognized as natural antioxidants. In this study, the potential of RDE to prevent cardiovascular diseases (CVDs) was evaluated using Caco-2 cells and a co-culture model of Caco-2 BBe1/EA.hy926 cells in Transwell<sup>®</sup> plates. The results showed that RDE supplementation significantly prevented interleukin-8 (IL-8) production and suppressed the gene expression of IL-8, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and cyclooxygenase 2 (COX-2) in the TNF-α inflamed Caco-2 cells. Meanwhile, the polyphenols (quercetin-3-glucoside, quercetin-glucuronide, rutin, quercetin-3-<i>O</i>-malonylglucoside, and kaempferol-glucoside) in the RDE were validated to be absorbed by Caco-2 BBe1 cells and transported to the basal chamber where EA.hy926 cells were located during 12 h incubation. The transported polyphenols were able to prevent IL-8 production and suppress the gene expression of proinflammatory mediators (TNF-α, ICAM-1, VCAM-1, and COX-2) in the TNF-α or oxidized low-density lipoprotein (ox-LDL) treated EA.hy926 cells. These novel findings demonstrated that phenolic compounds in RDE can be transported to the cardiovascular system by intestinal absorption and mitigate the inflammatory responses of vascular endothelial cells, indicating that RDE could be a natural resource of polyphenols to prevent inflammation cytokine or oxidized lipid-induced CVDs.https://www.mdpi.com/2076-3921/8/10/428red-osier dogwoodpolyphenolsantioxidantstnf-αox-ldlcaco-2 bbe-1/ea.hy926 co-culture cell model |
| spellingShingle | Qian Jiang Hua Zhang Runqiang Yang Qianru Hui Yuhuan Chen Lili Mats Rong Tsao Chengbo Yang Red-Osier Dogwood Extracts Prevent Inflammatory Responses in Caco-2 Cells and a Caco-2 BBe1/EA.hy926 Cell Co-Culture Model Antioxidants red-osier dogwood polyphenols antioxidants tnf-α ox-ldl caco-2 bbe-1/ea.hy926 co-culture cell model |
| title | Red-Osier Dogwood Extracts Prevent Inflammatory Responses in Caco-2 Cells and a Caco-2 BBe1/EA.hy926 Cell Co-Culture Model |
| title_full | Red-Osier Dogwood Extracts Prevent Inflammatory Responses in Caco-2 Cells and a Caco-2 BBe1/EA.hy926 Cell Co-Culture Model |
| title_fullStr | Red-Osier Dogwood Extracts Prevent Inflammatory Responses in Caco-2 Cells and a Caco-2 BBe1/EA.hy926 Cell Co-Culture Model |
| title_full_unstemmed | Red-Osier Dogwood Extracts Prevent Inflammatory Responses in Caco-2 Cells and a Caco-2 BBe1/EA.hy926 Cell Co-Culture Model |
| title_short | Red-Osier Dogwood Extracts Prevent Inflammatory Responses in Caco-2 Cells and a Caco-2 BBe1/EA.hy926 Cell Co-Culture Model |
| title_sort | red osier dogwood extracts prevent inflammatory responses in caco 2 cells and a caco 2 bbe1 ea hy926 cell co culture model |
| topic | red-osier dogwood polyphenols antioxidants tnf-α ox-ldl caco-2 bbe-1/ea.hy926 co-culture cell model |
| url | https://www.mdpi.com/2076-3921/8/10/428 |
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