An Aptamer-based Biosensor for Troponin I Detection in Diagnosis of Myocardial Infarction
Background: Acute myocardial infarction (MI) accounts for one third of deaths. Cardiac troponin I (TnI) is a reliable biomarker of cardiac muscle tissue injury and is employed in the early diagnosis of MI. Objectives: In this study, a molecular method is introduced to early diagnosis of MI by rap...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shiraz University of Medical Sciences
2018-06-01
|
| Series: | Journal of Biomedical Physics and Engineering |
| Subjects: | |
| Online Access: | http://www.jbpe.org/Journal_OJS/JBPE/index.php/jbpe/article/view/930 |
| Summary: | Background: Acute myocardial infarction (MI) accounts for one third of deaths. Cardiac troponin I (TnI) is a reliable biomarker of cardiac muscle tissue injury and is employed in the early diagnosis of MI.
Objectives: In this study, a molecular method is introduced to early diagnosis of MI by rapid detection of TnI.
Materials and Methods: The detection method was based on electrochemical aptasensing, being developed using different methods and evaluation steps. A gold electrode was used as a transducer to successful immobilize 76base aptamer to fabricate a TnI biosensor.
Results: The designed aptasensor could detect TnI in a range of 0.03 to 2.0 ng mL-1 without using any label, pre-concentration or amplification steps. The limit of detection was attained as 10 pg mL-1 without significant trouble of interfering species. The TnI biosensor demonestrated a stable, regenerative and reproducible function. 89 human samples were used to evaluate the performance of the TnI biosensor, and it represented 100% and 81%, diagnostic sensitivity and specificity, respectively.
Conclusions: This aptasensor may be used as an applicable tool in the future of early medical diagnosis of MI . |
|---|---|
| ISSN: | 2251-7200 2251-7200 |