Adaptive expression responses in the Pol-γ null strain of <it>S. pombe </it>depleted of mitochondrial genome
<p>Abstract</p> <p>Background</p> <p>DNA polymerase γ(Pol-γ) has been shown to be essential for maintenance of the mitochondrial genome (mtDNA) in the petite-positive budding yeast <it>Saccharomyces cerevisiae</it>. Budding yeast cells lacking mitochondria e...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2007-09-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/8/323 |
| _version_ | 1818752313438765056 |
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| author | Karuturi R Krishna Eshaghi Majid Li Juntao Chu Zhaoqing Lin Kui Liu Jianhua |
| author_facet | Karuturi R Krishna Eshaghi Majid Li Juntao Chu Zhaoqing Lin Kui Liu Jianhua |
| author_sort | Karuturi R Krishna |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>DNA polymerase γ(Pol-γ) has been shown to be essential for maintenance of the mitochondrial genome (mtDNA) in the petite-positive budding yeast <it>Saccharomyces cerevisiae</it>. Budding yeast cells lacking mitochondria exhibit a slow-growing or petite-colony phenotype. Petite strains fail to grow on non-fermentable carbon sources. However, it is not clear whether the Pol-γ is required for mtDNA maintenance in the petite-negative fission yeast <it>Schizosaccharomyces pombe</it>.</p> <p>Results</p> <p>We show that disruption of the nuclear gene <it>pog1</it><sup>+ </sup>that encodes Pol-γ is sufficient to deplete mtDNA in <it>S. pombe</it>. Cells bearing <it>pog1Δ </it>allele require substantial growth periods to form petite colonies. Mitotracker assays indicate that <it>pog1Δ </it>cells are defective in mitochondrial function and EM analyses suggest that <it>pog1Δ </it>cells lack normal mitochondrial structures. Depletion of mtDNA in <it>pog1Δ </it>cells is evident from quantitative real-time PCR assays. Genome-wide expression profiles of <it>pog1Δ </it>and other mtDNA-less cells reveal that many genes involved in response to stimulus, energy derivation by oxidation of organic compounds, cellular carbohydrate metabolism, and energy reserve metabolism are induced. Conversely, many genes encoding proteins involved in amino acid metabolism and oxidative phosphorylation are repressed.</p> <p>Conclusion</p> <p>By showing that Pol-γ is essential for mtDNA maintenance and disruption of <it>pog1</it><sup>+ </sup>alters the genome-wide expression profiles, we demonstrated that cells lacking mtDNA exhibit adaptive nuclear gene expression responses in the petite-negative <it>S. pombe</it>.</p> |
| first_indexed | 2024-12-18T04:49:29Z |
| format | Article |
| id | doaj.art-cb436b36214542b2bf020f3d7f76e745 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-18T04:49:29Z |
| publishDate | 2007-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-cb436b36214542b2bf020f3d7f76e7452022-12-21T21:20:28ZengBMCBMC Genomics1471-21642007-09-018132310.1186/1471-2164-8-323Adaptive expression responses in the Pol-γ null strain of <it>S. pombe </it>depleted of mitochondrial genomeKaruturi R KrishnaEshaghi MajidLi JuntaoChu ZhaoqingLin KuiLiu Jianhua<p>Abstract</p> <p>Background</p> <p>DNA polymerase γ(Pol-γ) has been shown to be essential for maintenance of the mitochondrial genome (mtDNA) in the petite-positive budding yeast <it>Saccharomyces cerevisiae</it>. Budding yeast cells lacking mitochondria exhibit a slow-growing or petite-colony phenotype. Petite strains fail to grow on non-fermentable carbon sources. However, it is not clear whether the Pol-γ is required for mtDNA maintenance in the petite-negative fission yeast <it>Schizosaccharomyces pombe</it>.</p> <p>Results</p> <p>We show that disruption of the nuclear gene <it>pog1</it><sup>+ </sup>that encodes Pol-γ is sufficient to deplete mtDNA in <it>S. pombe</it>. Cells bearing <it>pog1Δ </it>allele require substantial growth periods to form petite colonies. Mitotracker assays indicate that <it>pog1Δ </it>cells are defective in mitochondrial function and EM analyses suggest that <it>pog1Δ </it>cells lack normal mitochondrial structures. Depletion of mtDNA in <it>pog1Δ </it>cells is evident from quantitative real-time PCR assays. Genome-wide expression profiles of <it>pog1Δ </it>and other mtDNA-less cells reveal that many genes involved in response to stimulus, energy derivation by oxidation of organic compounds, cellular carbohydrate metabolism, and energy reserve metabolism are induced. Conversely, many genes encoding proteins involved in amino acid metabolism and oxidative phosphorylation are repressed.</p> <p>Conclusion</p> <p>By showing that Pol-γ is essential for mtDNA maintenance and disruption of <it>pog1</it><sup>+ </sup>alters the genome-wide expression profiles, we demonstrated that cells lacking mtDNA exhibit adaptive nuclear gene expression responses in the petite-negative <it>S. pombe</it>.</p>http://www.biomedcentral.com/1471-2164/8/323 |
| spellingShingle | Karuturi R Krishna Eshaghi Majid Li Juntao Chu Zhaoqing Lin Kui Liu Jianhua Adaptive expression responses in the Pol-γ null strain of <it>S. pombe </it>depleted of mitochondrial genome BMC Genomics |
| title | Adaptive expression responses in the Pol-γ null strain of <it>S. pombe </it>depleted of mitochondrial genome |
| title_full | Adaptive expression responses in the Pol-γ null strain of <it>S. pombe </it>depleted of mitochondrial genome |
| title_fullStr | Adaptive expression responses in the Pol-γ null strain of <it>S. pombe </it>depleted of mitochondrial genome |
| title_full_unstemmed | Adaptive expression responses in the Pol-γ null strain of <it>S. pombe </it>depleted of mitochondrial genome |
| title_short | Adaptive expression responses in the Pol-γ null strain of <it>S. pombe </it>depleted of mitochondrial genome |
| title_sort | adaptive expression responses in the pol γ null strain of it s pombe it depleted of mitochondrial genome |
| url | http://www.biomedcentral.com/1471-2164/8/323 |
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