Hyperoside Attenuate Inflammation in HT22 Cells via Upregulating SIRT1 to Activities Wnt/β-Catenin and Sonic Hedgehog Pathways
Neuroinflammation plays important roles in the pathogenesis and progression of altered neurodevelopment, sensorineural hearing loss, and certain neurodegenerative diseases. Hyperoside (quercetin-3-O-β-D-galactoside) is an active compound isolated from Hypericum plants. In this study, we investigate...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2021-01-01
|
| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2021/8706400 |
| _version_ | 1798031703302733824 |
|---|---|
| author | Jin Huang Liang Zhou Jilin Chen Tingbao Chen Bo Lei Niandong Zheng Xiaoqiang Wan Jianguo Xu Tinghua Wang |
| author_facet | Jin Huang Liang Zhou Jilin Chen Tingbao Chen Bo Lei Niandong Zheng Xiaoqiang Wan Jianguo Xu Tinghua Wang |
| author_sort | Jin Huang |
| collection | DOAJ |
| description | Neuroinflammation plays important roles in the pathogenesis and progression of altered neurodevelopment, sensorineural hearing loss, and certain neurodegenerative diseases. Hyperoside (quercetin-3-O-β-D-galactoside) is an active compound isolated from Hypericum plants. In this study, we investigate the protective effect of hyperoside on neuroinflammation and its possible molecular mechanism. Lipopolysaccharide (LPS) and hyperoside were used to treat HT22 cells. The cell viability was measured by MTT assay. The cell apoptosis rate was measured by flow cytometry assay. The mRNA expression levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α) were determined by quantitative reverse transcription polymerase chain reaction. The levels of oxidative stress indices superoxide dismutase (SOD), reactive oxygen species (ROS), catalase (CAT), glutathione (GSH), and malondialdehyde (MDA) were measured by the kits. The expression of neurotrophic factor and the relationship among hyperoside, silent mating type information regulation 2 homolog-1 (SIRT1) and Wnt/β-catenin, and sonic hedgehog was examined by western blotting. In the LPS-induced HT22 cells, hyperoside promotes cell survival; alleviates the level of IL-1β, IL-6, IL-8, TNF-α, ROS, MDA, Bax, and caspase-3; and increases the expression of CAT, SOD, GSH, Bcl-2, BDNF, TrkB, and NGF. In addition, hyperoside upregulated the expression of SIRT1. Further mechanistic investigation showed that hyperoside alleviated LPS-induced inflammation, oxidative stress, and apoptosis by upregulating SIRT1 to activate Wnt/β-catenin and sonic hedgehog pathways. Taken together, our data suggested that hyperoside acts as a protector in neuroinflammation. |
| first_indexed | 2024-04-11T20:01:40Z |
| format | Article |
| id | doaj.art-cb44265344a9484cbc504cffd020073f |
| institution | Directory Open Access Journal |
| issn | 2090-5904 1687-5443 |
| language | English |
| last_indexed | 2024-04-11T20:01:40Z |
| publishDate | 2021-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj.art-cb44265344a9484cbc504cffd020073f2022-12-22T04:05:36ZengHindawi LimitedNeural Plasticity2090-59041687-54432021-01-01202110.1155/2021/87064008706400Hyperoside Attenuate Inflammation in HT22 Cells via Upregulating SIRT1 to Activities Wnt/β-Catenin and Sonic Hedgehog PathwaysJin Huang0Liang Zhou1Jilin Chen2Tingbao Chen3Bo Lei4Niandong Zheng5Xiaoqiang Wan6Jianguo Xu7Tinghua Wang8Institute of Neuroscience, Basic Medical College, Kunming Medical University, Kunming, Yunnan 650500, ChinaDepartment of Neurology, Zhenxiong County People Hospital, Zhaotong, Yunnan Province, ChinaDepartment of Animal Zoology, Kunming Medical University, Kunming, Yunnan Province, ChinaDepartment of Animal Zoology, Kunming Medical University, Kunming, Yunnan Province, ChinaDepartment of Neurosurgery, West-China Hospital, Sichuan University, Chengdu, Sichuan 610041, ChinaNeurosurgery of the People’s Hospital of Leshan, Leshan 614000, ChinaNeurosurgery of the People’s Hospital of Leshan, Leshan 614000, ChinaDepartment of Neurosurgery, West-China Hospital, Sichuan University, Chengdu, Sichuan 610041, ChinaInstitute of Neuroscience, Basic Medical College, Kunming Medical University, Kunming, Yunnan 650500, ChinaNeuroinflammation plays important roles in the pathogenesis and progression of altered neurodevelopment, sensorineural hearing loss, and certain neurodegenerative diseases. Hyperoside (quercetin-3-O-β-D-galactoside) is an active compound isolated from Hypericum plants. In this study, we investigate the protective effect of hyperoside on neuroinflammation and its possible molecular mechanism. Lipopolysaccharide (LPS) and hyperoside were used to treat HT22 cells. The cell viability was measured by MTT assay. The cell apoptosis rate was measured by flow cytometry assay. The mRNA expression levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α) were determined by quantitative reverse transcription polymerase chain reaction. The levels of oxidative stress indices superoxide dismutase (SOD), reactive oxygen species (ROS), catalase (CAT), glutathione (GSH), and malondialdehyde (MDA) were measured by the kits. The expression of neurotrophic factor and the relationship among hyperoside, silent mating type information regulation 2 homolog-1 (SIRT1) and Wnt/β-catenin, and sonic hedgehog was examined by western blotting. In the LPS-induced HT22 cells, hyperoside promotes cell survival; alleviates the level of IL-1β, IL-6, IL-8, TNF-α, ROS, MDA, Bax, and caspase-3; and increases the expression of CAT, SOD, GSH, Bcl-2, BDNF, TrkB, and NGF. In addition, hyperoside upregulated the expression of SIRT1. Further mechanistic investigation showed that hyperoside alleviated LPS-induced inflammation, oxidative stress, and apoptosis by upregulating SIRT1 to activate Wnt/β-catenin and sonic hedgehog pathways. Taken together, our data suggested that hyperoside acts as a protector in neuroinflammation.http://dx.doi.org/10.1155/2021/8706400 |
| spellingShingle | Jin Huang Liang Zhou Jilin Chen Tingbao Chen Bo Lei Niandong Zheng Xiaoqiang Wan Jianguo Xu Tinghua Wang Hyperoside Attenuate Inflammation in HT22 Cells via Upregulating SIRT1 to Activities Wnt/β-Catenin and Sonic Hedgehog Pathways Neural Plasticity |
| title | Hyperoside Attenuate Inflammation in HT22 Cells via Upregulating SIRT1 to Activities Wnt/β-Catenin and Sonic Hedgehog Pathways |
| title_full | Hyperoside Attenuate Inflammation in HT22 Cells via Upregulating SIRT1 to Activities Wnt/β-Catenin and Sonic Hedgehog Pathways |
| title_fullStr | Hyperoside Attenuate Inflammation in HT22 Cells via Upregulating SIRT1 to Activities Wnt/β-Catenin and Sonic Hedgehog Pathways |
| title_full_unstemmed | Hyperoside Attenuate Inflammation in HT22 Cells via Upregulating SIRT1 to Activities Wnt/β-Catenin and Sonic Hedgehog Pathways |
| title_short | Hyperoside Attenuate Inflammation in HT22 Cells via Upregulating SIRT1 to Activities Wnt/β-Catenin and Sonic Hedgehog Pathways |
| title_sort | hyperoside attenuate inflammation in ht22 cells via upregulating sirt1 to activities wnt β catenin and sonic hedgehog pathways |
| url | http://dx.doi.org/10.1155/2021/8706400 |
| work_keys_str_mv | AT jinhuang hyperosideattenuateinflammationinht22cellsviaupregulatingsirt1toactivitieswntbcateninandsonichedgehogpathways AT liangzhou hyperosideattenuateinflammationinht22cellsviaupregulatingsirt1toactivitieswntbcateninandsonichedgehogpathways AT jilinchen hyperosideattenuateinflammationinht22cellsviaupregulatingsirt1toactivitieswntbcateninandsonichedgehogpathways AT tingbaochen hyperosideattenuateinflammationinht22cellsviaupregulatingsirt1toactivitieswntbcateninandsonichedgehogpathways AT bolei hyperosideattenuateinflammationinht22cellsviaupregulatingsirt1toactivitieswntbcateninandsonichedgehogpathways AT niandongzheng hyperosideattenuateinflammationinht22cellsviaupregulatingsirt1toactivitieswntbcateninandsonichedgehogpathways AT xiaoqiangwan hyperosideattenuateinflammationinht22cellsviaupregulatingsirt1toactivitieswntbcateninandsonichedgehogpathways AT jianguoxu hyperosideattenuateinflammationinht22cellsviaupregulatingsirt1toactivitieswntbcateninandsonichedgehogpathways AT tinghuawang hyperosideattenuateinflammationinht22cellsviaupregulatingsirt1toactivitieswntbcateninandsonichedgehogpathways |