Luteolin decreases the pathogenicity of Aeromonas hydrophila via inhibiting the activity of aerolysin
Aeromonas hydrophila (A. hydrophila) can cause a number of diseases in both human and animals. A. hydrophila-related infections in aquaculture cause severe economic losses every year throughout the world. The emergence of antibiotic resistance that is due to the abuse of antibiotics has limited the...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2021-12-01
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| Series: | Virulence |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21505594.2020.1867455 |
| _version_ | 1828125669606817792 |
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| author | Jing Dong Lushan Zhang Yongtao Liu Ning Xu Shun Zhou Yibin Yang Qiuhong Yang Xiaohui Ai |
| author_facet | Jing Dong Lushan Zhang Yongtao Liu Ning Xu Shun Zhou Yibin Yang Qiuhong Yang Xiaohui Ai |
| author_sort | Jing Dong |
| collection | DOAJ |
| description | Aeromonas hydrophila (A. hydrophila) can cause a number of diseases in both human and animals. A. hydrophila-related infections in aquaculture cause severe economic losses every year throughout the world. The emergence of antibiotic resistance that is due to the abuse of antibiotics has limited the application of antibiotics. Thus, novel approaches are needed to combat with treatment failure of antibiotics caused by resistant bacterial strains. Aerolysin plays a critical role in the pathogenesis of A. hydrophila and has been considered as a novel target for developing drugs based on anti-virulence strategies. Here, we reported that luteolin, a natural product with no anti-A. hydrophila activity, could reduce aerolysin-induced hemolysis by inhibiting aerolysin activity. The binding mode was simulated by molecular docking and dynamics simulation. Then the main binding sites were confirmed by fluorescence quenching assays. We found that luteolin could hindered the formation of functional heptamer of aerolysin according to the results of the oligomerization assay. Moreover, luteolin could protect A549 cells from aerolysin mediated cell death and increase the survival rate of A. hydrophila-infected channel catfish. These findings suggest a novel approach to developing drugs fighting against A. hydrophila, and luteolin can be a promising drug candidate for treatment of A. hydrophila-associated infections. |
| first_indexed | 2024-04-11T15:24:02Z |
| format | Article |
| id | doaj.art-cb48371fd8dc470a82a69c6836309546 |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-04-11T15:24:02Z |
| publishDate | 2021-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-cb48371fd8dc470a82a69c68363095462022-12-22T04:16:18ZengTaylor & Francis GroupVirulence2150-55942150-56082021-12-0112116517610.1080/21505594.2020.18674551867455Luteolin decreases the pathogenicity of Aeromonas hydrophila via inhibiting the activity of aerolysinJing Dong0Lushan Zhang1Yongtao Liu2Ning Xu3Shun Zhou4Yibin Yang5Qiuhong Yang6Xiaohui Ai7Chinese Academy of Fishery SciencesChinese Academy of Fishery SciencesChinese Academy of Fishery SciencesChinese Academy of Fishery SciencesChinese Academy of Fishery SciencesChinese Academy of Fishery SciencesChinese Academy of Fishery SciencesChinese Academy of Fishery SciencesAeromonas hydrophila (A. hydrophila) can cause a number of diseases in both human and animals. A. hydrophila-related infections in aquaculture cause severe economic losses every year throughout the world. The emergence of antibiotic resistance that is due to the abuse of antibiotics has limited the application of antibiotics. Thus, novel approaches are needed to combat with treatment failure of antibiotics caused by resistant bacterial strains. Aerolysin plays a critical role in the pathogenesis of A. hydrophila and has been considered as a novel target for developing drugs based on anti-virulence strategies. Here, we reported that luteolin, a natural product with no anti-A. hydrophila activity, could reduce aerolysin-induced hemolysis by inhibiting aerolysin activity. The binding mode was simulated by molecular docking and dynamics simulation. Then the main binding sites were confirmed by fluorescence quenching assays. We found that luteolin could hindered the formation of functional heptamer of aerolysin according to the results of the oligomerization assay. Moreover, luteolin could protect A549 cells from aerolysin mediated cell death and increase the survival rate of A. hydrophila-infected channel catfish. These findings suggest a novel approach to developing drugs fighting against A. hydrophila, and luteolin can be a promising drug candidate for treatment of A. hydrophila-associated infections.http://dx.doi.org/10.1080/21505594.2020.1867455aeromonas hydrophilaaerolysinluteolinanti-virulencemolecular dynamics |
| spellingShingle | Jing Dong Lushan Zhang Yongtao Liu Ning Xu Shun Zhou Yibin Yang Qiuhong Yang Xiaohui Ai Luteolin decreases the pathogenicity of Aeromonas hydrophila via inhibiting the activity of aerolysin Virulence aeromonas hydrophila aerolysin luteolin anti-virulence molecular dynamics |
| title | Luteolin decreases the pathogenicity of Aeromonas hydrophila via inhibiting the activity of aerolysin |
| title_full | Luteolin decreases the pathogenicity of Aeromonas hydrophila via inhibiting the activity of aerolysin |
| title_fullStr | Luteolin decreases the pathogenicity of Aeromonas hydrophila via inhibiting the activity of aerolysin |
| title_full_unstemmed | Luteolin decreases the pathogenicity of Aeromonas hydrophila via inhibiting the activity of aerolysin |
| title_short | Luteolin decreases the pathogenicity of Aeromonas hydrophila via inhibiting the activity of aerolysin |
| title_sort | luteolin decreases the pathogenicity of aeromonas hydrophila via inhibiting the activity of aerolysin |
| topic | aeromonas hydrophila aerolysin luteolin anti-virulence molecular dynamics |
| url | http://dx.doi.org/10.1080/21505594.2020.1867455 |
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