A combined treatment of Proteinase K and biosynthesized ZnO-NPs for eradication of dairy biofilm of sporeformers
Biofilms of sporeformers found in the dairy industry are the major contaminants during processing, as they withstand heat and chemical treatment that are used to control microbes. The present work is aimed to remove these resistant forms of bacterial community (biofilm) present in dairy production l...
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| Format: | Article |
| Language: | English |
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AIMS Press
2022-12-01
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| Series: | AIMS Microbiology |
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| Online Access: | https://www.aimspress.com/article/doi/10.3934/microbiol.2022033?viewType=HTML |
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| author | Ahmed A. Radwan Osama M. Darwesh Maha T. Emam Karima A. Mohamed Hala M. Abu Shady |
| author_facet | Ahmed A. Radwan Osama M. Darwesh Maha T. Emam Karima A. Mohamed Hala M. Abu Shady |
| author_sort | Ahmed A. Radwan |
| collection | DOAJ |
| description | Biofilms of sporeformers found in the dairy industry are the major contaminants during processing, as they withstand heat and chemical treatment that are used to control microbes. The present work is aimed to remove these resistant forms of bacterial community (biofilm) present in dairy production lines using ecofriendly agents based on proteinase K (Prot-K) coupled with Zinc oxide nanoparticles (ZnO-NPs). Some metal/metal oxide (Ag, CuO and ZnO) NPs were prepared microbially, and ZnO-NPs were characterized as the most effective ones among them. The produced ZnO-NPs were 15–25 nm in size with spherical shape, and FTIR analysis confirmed the presence of proteins and alkanes surrounding particles as capping agents. Application of Prot-K for eradication (removal) of a model biofilm of mixed sporeformers on food-grade stainless steel resulted in an 83% reduction in the absorbance of crystal violet-stained biofilm. When Prot-K was mixed with the biosynthesized NPs ZnO_G240, the reduction increased to 99.19%. This finding could contribute to an efficient cleaning approach combined with CIP to remove the recalcitrant biofilms in dairy production lines. |
| first_indexed | 2024-04-11T00:13:37Z |
| format | Article |
| id | doaj.art-cb48a3c242fa4242aeff038461a365a4 |
| institution | Directory Open Access Journal |
| issn | 2471-1888 |
| language | English |
| last_indexed | 2024-04-11T00:13:37Z |
| publishDate | 2022-12-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | AIMS Microbiology |
| spelling | doaj.art-cb48a3c242fa4242aeff038461a365a42023-01-09T06:00:15ZengAIMS PressAIMS Microbiology2471-18882022-12-018450752710.3934/microbiol.2022033A combined treatment of Proteinase K and biosynthesized ZnO-NPs for eradication of dairy biofilm of sporeformersAhmed A. Radwan 0Osama M. Darwesh1Maha T. Emam2Karima A. Mohamed3Hala M. Abu Shady 41. Genetics and Cytology Dept., National Research Centre (NRC), Cairo, 12622, Egypt2. Agricultural Microbiology Dept., National Research Centre (NRC), Cairo, 12622, Egypt1. Genetics and Cytology Dept., National Research Centre (NRC), Cairo, 12622, Egypt1. Genetics and Cytology Dept., National Research Centre (NRC), Cairo, 12622, Egypt3. Microbiology Dept., Faculty of Science, Ain-Shams University Cairo, EgyptBiofilms of sporeformers found in the dairy industry are the major contaminants during processing, as they withstand heat and chemical treatment that are used to control microbes. The present work is aimed to remove these resistant forms of bacterial community (biofilm) present in dairy production lines using ecofriendly agents based on proteinase K (Prot-K) coupled with Zinc oxide nanoparticles (ZnO-NPs). Some metal/metal oxide (Ag, CuO and ZnO) NPs were prepared microbially, and ZnO-NPs were characterized as the most effective ones among them. The produced ZnO-NPs were 15–25 nm in size with spherical shape, and FTIR analysis confirmed the presence of proteins and alkanes surrounding particles as capping agents. Application of Prot-K for eradication (removal) of a model biofilm of mixed sporeformers on food-grade stainless steel resulted in an 83% reduction in the absorbance of crystal violet-stained biofilm. When Prot-K was mixed with the biosynthesized NPs ZnO_G240, the reduction increased to 99.19%. This finding could contribute to an efficient cleaning approach combined with CIP to remove the recalcitrant biofilms in dairy production lines.https://www.aimspress.com/article/doi/10.3934/microbiol.2022033?viewType=HTMLdairy sporeformersbiofilm eradicationproteinase kbiosynthesized zno-nps |
| spellingShingle | Ahmed A. Radwan Osama M. Darwesh Maha T. Emam Karima A. Mohamed Hala M. Abu Shady A combined treatment of Proteinase K and biosynthesized ZnO-NPs for eradication of dairy biofilm of sporeformers AIMS Microbiology dairy sporeformers biofilm eradication proteinase k biosynthesized zno-nps |
| title | A combined treatment of Proteinase K and biosynthesized ZnO-NPs for eradication of dairy biofilm of sporeformers |
| title_full | A combined treatment of Proteinase K and biosynthesized ZnO-NPs for eradication of dairy biofilm of sporeformers |
| title_fullStr | A combined treatment of Proteinase K and biosynthesized ZnO-NPs for eradication of dairy biofilm of sporeformers |
| title_full_unstemmed | A combined treatment of Proteinase K and biosynthesized ZnO-NPs for eradication of dairy biofilm of sporeformers |
| title_short | A combined treatment of Proteinase K and biosynthesized ZnO-NPs for eradication of dairy biofilm of sporeformers |
| title_sort | combined treatment of proteinase k and biosynthesized zno nps for eradication of dairy biofilm of sporeformers |
| topic | dairy sporeformers biofilm eradication proteinase k biosynthesized zno-nps |
| url | https://www.aimspress.com/article/doi/10.3934/microbiol.2022033?viewType=HTML |
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