Inhibitory Effects of Curcumin and 2-Methoxyestradiaul on Anaphase Promoting Complex in Hela Cancer Cells
Background: Anaphase promoting complex (APC) plays a critical role in cell division and mitotic exit. This protein complex may have a pivotal role in the cell cycle control affecting pathological conditions such as cancer. APC is recommended as the target of many anti-cancer agents due to its import...
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Isfahan University of Medical Sciences
2013-06-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/2680 |
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| author | Hamzeh Rahimi Alireza Foroumadi Mohammad Ali Shokrgozar Reza Mahdian Armin Madadkar-Sobhani Mortaza Karimipoor |
| author_facet | Hamzeh Rahimi Alireza Foroumadi Mohammad Ali Shokrgozar Reza Mahdian Armin Madadkar-Sobhani Mortaza Karimipoor |
| author_sort | Hamzeh Rahimi |
| collection | DOAJ |
| description | Background: Anaphase promoting complex (APC) plays a critical role in cell division and mitotic exit. This protein complex may have a pivotal role in the cell cycle control affecting pathological conditions such as cancer. APC is recommended as the target of many anti-cancer agents due to its importance in cancer pathogenesis. This study aimed to evaluate the inhibitory effects of curcumin and 2-methoxyestradiaul on APC.
Methods: Hela cells were treated with various concentrations of curcumin and 2-methoxyestradiaul, and their cytotoxic effects were investigated after 24, 48, 72 and 96 hours by MTT assay. Expression of securin, cyclin B and the APC substrates were investigated using immune-blotting. Finally, cell cycle analysis was performed using DRAQ5 staining and flowcytometry.
Findings: Respectively, 20 and 30 percent of cell-death after 24-hour treatment with curcumin and 2-methoxyestradiaul was seen. Also, securin and cyclin levels were raised after 24 hours of treatment. Furthermore, the levels of the substrates (securin and cyclin B) increased 48 hours after treatment. Results of fluorescence-activated cell sorting (FACS) analysis showed that treated cells were arrested in G2 phase of cell cycle which revealed cell arrest in G2 phase of cell cycle. 2-methoxyestradiaul showed a better APC inhibition effect than curcumin (P = 0.02).
Conclusions: Our results revealed that APC is a suitable target for cancer treatment and curcumin and 2-methoxyestradiaul have inhibitory effects on the activity of this complex. |
| first_indexed | 2024-03-12T09:49:27Z |
| format | Article |
| id | doaj.art-cb49376fc7244670836fa94f3e7fcf2a |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T09:49:27Z |
| publishDate | 2013-06-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-cb49376fc7244670836fa94f3e7fcf2a2023-09-02T12:39:48ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2013-06-01312282552641300Inhibitory Effects of Curcumin and 2-Methoxyestradiaul on Anaphase Promoting Complex in Hela Cancer CellsHamzeh Rahimi0Alireza Foroumadi1Mohammad Ali Shokrgozar2Reza Mahdian3Armin Madadkar-Sobhani4Mortaza Karimipoor5PhD Student, Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IranProfessor, Department of Pharmaceutics, School of Pharmacy AND Pharmaceutical Science Research Center, Tehran University of Medical Sciences, Tehran, IranAssociate Professor, Department of Biotechnology, National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, IranAssistant Professor, Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IranAssistant Professor, Department of Life Sciences, Barcelona Supercomputing Center (BSC), Barcelona, SpainAssistant Professor, Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IranBackground: Anaphase promoting complex (APC) plays a critical role in cell division and mitotic exit. This protein complex may have a pivotal role in the cell cycle control affecting pathological conditions such as cancer. APC is recommended as the target of many anti-cancer agents due to its importance in cancer pathogenesis. This study aimed to evaluate the inhibitory effects of curcumin and 2-methoxyestradiaul on APC. Methods: Hela cells were treated with various concentrations of curcumin and 2-methoxyestradiaul, and their cytotoxic effects were investigated after 24, 48, 72 and 96 hours by MTT assay. Expression of securin, cyclin B and the APC substrates were investigated using immune-blotting. Finally, cell cycle analysis was performed using DRAQ5 staining and flowcytometry. Findings: Respectively, 20 and 30 percent of cell-death after 24-hour treatment with curcumin and 2-methoxyestradiaul was seen. Also, securin and cyclin levels were raised after 24 hours of treatment. Furthermore, the levels of the substrates (securin and cyclin B) increased 48 hours after treatment. Results of fluorescence-activated cell sorting (FACS) analysis showed that treated cells were arrested in G2 phase of cell cycle which revealed cell arrest in G2 phase of cell cycle. 2-methoxyestradiaul showed a better APC inhibition effect than curcumin (P = 0.02). Conclusions: Our results revealed that APC is a suitable target for cancer treatment and curcumin and 2-methoxyestradiaul have inhibitory effects on the activity of this complex.http://jims.mui.ac.ir/index.php/jims/article/view/2680Anaphase promoting complexCell cycleCell deathCancer |
| spellingShingle | Hamzeh Rahimi Alireza Foroumadi Mohammad Ali Shokrgozar Reza Mahdian Armin Madadkar-Sobhani Mortaza Karimipoor Inhibitory Effects of Curcumin and 2-Methoxyestradiaul on Anaphase Promoting Complex in Hela Cancer Cells مجله دانشکده پزشکی اصفهان Anaphase promoting complex Cell cycle Cell death Cancer |
| title | Inhibitory Effects of Curcumin and 2-Methoxyestradiaul on Anaphase Promoting Complex in Hela Cancer Cells |
| title_full | Inhibitory Effects of Curcumin and 2-Methoxyestradiaul on Anaphase Promoting Complex in Hela Cancer Cells |
| title_fullStr | Inhibitory Effects of Curcumin and 2-Methoxyestradiaul on Anaphase Promoting Complex in Hela Cancer Cells |
| title_full_unstemmed | Inhibitory Effects of Curcumin and 2-Methoxyestradiaul on Anaphase Promoting Complex in Hela Cancer Cells |
| title_short | Inhibitory Effects of Curcumin and 2-Methoxyestradiaul on Anaphase Promoting Complex in Hela Cancer Cells |
| title_sort | inhibitory effects of curcumin and 2 methoxyestradiaul on anaphase promoting complex in hela cancer cells |
| topic | Anaphase promoting complex Cell cycle Cell death Cancer |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/2680 |
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