New Genes Identified as Modulating Salt Tolerance in Maize Seedlings Using the Combination of Transcriptome Analysis and BSA
(1) Background: Salt stress is an abiotic factor that limits maize yield and quality. A highly salt-tolerance inbred AS5 and a salt-sensitive inbred NX420 collected from Ningxia Province, China, were used to identify new genes for modulating salt resistance in maize. (2) Methods: To understand the d...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/6/1331 |
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| author | Yongxing Zhu Ying Ren Ji’an Liu Wenguang Liang Yuanyuan Zhang Fengyuan Shen Jiang Ling Chunyi Zhang |
| author_facet | Yongxing Zhu Ying Ren Ji’an Liu Wenguang Liang Yuanyuan Zhang Fengyuan Shen Jiang Ling Chunyi Zhang |
| author_sort | Yongxing Zhu |
| collection | DOAJ |
| description | (1) Background: Salt stress is an abiotic factor that limits maize yield and quality. A highly salt-tolerance inbred AS5 and a salt-sensitive inbred NX420 collected from Ningxia Province, China, were used to identify new genes for modulating salt resistance in maize. (2) Methods: To understand the different molecular bases of salt tolerance in AS5 and NX420, we performed BSA-seq using an F2 population for two extreme bulks derived from the cross between AS5 and NX420. Transcriptomic analysis was also conducted for AS5 and NX420 at the seedling stage after treatment with 150 mM of NaCl for 14 days. (3) Results: AS5 had a higher biomass and lower Na<sup>+</sup> content than NX420 in the seedling stage after treatment with 150 mM NaCl for 14 days. One hundred and six candidate regions for salt tolerance were mapped on all of the chromosomes through BSA-seq using F2 in an extreme population. Based on the polymorphisms identified between both parents, we detected 77 genes. A large number of differentially expressed genes (DEGs) at the seedling stage under salt stress between these two inbred lines were detected using transcriptome sequencing. GO analysis indicated that 925 and 686 genes were significantly enriched in the integral component of the membrane of AS5 and NX420, respectively. Among these results, two and four DEGs were identified as overlapping in these two inbred lines using BSA-seq and transcriptomic analysis, respectively. Two genes (<i>Zm00001d053925</i> and <i>Zm00001d037181</i>) were detected in both AS5 and NX420; the transcription level of <i>Zm00001d053925</i> was induced to be significantly higher in AS5 than in NX420 (41.99 times versus 6.06 times after 150 mM of NaCl treatment for 48 h), while the expression of <i>Zm00001d037181</i> showed no significant difference upon salt treatment in both lines. The functional annotation of the new candidate genes showed that it was an unknown function protein. (4) Conclusions: <i>Zm00001d053925</i> is a new functional gene responding to salt stress in the seedling stage, which provides an important genetic resource for salt-tolerant maize breeding. |
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| language | English |
| last_indexed | 2024-03-11T06:00:36Z |
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| spelling | doaj.art-cb4bb837b696481f9fd954bb1751b9782023-11-17T13:22:24ZengMDPI AGPlants2223-77472023-03-01126133110.3390/plants12061331New Genes Identified as Modulating Salt Tolerance in Maize Seedlings Using the Combination of Transcriptome Analysis and BSAYongxing Zhu0Ying Ren1Ji’an Liu2Wenguang Liang3Yuanyuan Zhang4Fengyuan Shen5Jiang Ling6Chunyi Zhang7Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China(1) Background: Salt stress is an abiotic factor that limits maize yield and quality. A highly salt-tolerance inbred AS5 and a salt-sensitive inbred NX420 collected from Ningxia Province, China, were used to identify new genes for modulating salt resistance in maize. (2) Methods: To understand the different molecular bases of salt tolerance in AS5 and NX420, we performed BSA-seq using an F2 population for two extreme bulks derived from the cross between AS5 and NX420. Transcriptomic analysis was also conducted for AS5 and NX420 at the seedling stage after treatment with 150 mM of NaCl for 14 days. (3) Results: AS5 had a higher biomass and lower Na<sup>+</sup> content than NX420 in the seedling stage after treatment with 150 mM NaCl for 14 days. One hundred and six candidate regions for salt tolerance were mapped on all of the chromosomes through BSA-seq using F2 in an extreme population. Based on the polymorphisms identified between both parents, we detected 77 genes. A large number of differentially expressed genes (DEGs) at the seedling stage under salt stress between these two inbred lines were detected using transcriptome sequencing. GO analysis indicated that 925 and 686 genes were significantly enriched in the integral component of the membrane of AS5 and NX420, respectively. Among these results, two and four DEGs were identified as overlapping in these two inbred lines using BSA-seq and transcriptomic analysis, respectively. Two genes (<i>Zm00001d053925</i> and <i>Zm00001d037181</i>) were detected in both AS5 and NX420; the transcription level of <i>Zm00001d053925</i> was induced to be significantly higher in AS5 than in NX420 (41.99 times versus 6.06 times after 150 mM of NaCl treatment for 48 h), while the expression of <i>Zm00001d037181</i> showed no significant difference upon salt treatment in both lines. The functional annotation of the new candidate genes showed that it was an unknown function protein. (4) Conclusions: <i>Zm00001d053925</i> is a new functional gene responding to salt stress in the seedling stage, which provides an important genetic resource for salt-tolerant maize breeding.https://www.mdpi.com/2223-7747/12/6/1331salt-tolerantBSA-seqtranscriptomic analysismaize inbred |
| spellingShingle | Yongxing Zhu Ying Ren Ji’an Liu Wenguang Liang Yuanyuan Zhang Fengyuan Shen Jiang Ling Chunyi Zhang New Genes Identified as Modulating Salt Tolerance in Maize Seedlings Using the Combination of Transcriptome Analysis and BSA Plants salt-tolerant BSA-seq transcriptomic analysis maize inbred |
| title | New Genes Identified as Modulating Salt Tolerance in Maize Seedlings Using the Combination of Transcriptome Analysis and BSA |
| title_full | New Genes Identified as Modulating Salt Tolerance in Maize Seedlings Using the Combination of Transcriptome Analysis and BSA |
| title_fullStr | New Genes Identified as Modulating Salt Tolerance in Maize Seedlings Using the Combination of Transcriptome Analysis and BSA |
| title_full_unstemmed | New Genes Identified as Modulating Salt Tolerance in Maize Seedlings Using the Combination of Transcriptome Analysis and BSA |
| title_short | New Genes Identified as Modulating Salt Tolerance in Maize Seedlings Using the Combination of Transcriptome Analysis and BSA |
| title_sort | new genes identified as modulating salt tolerance in maize seedlings using the combination of transcriptome analysis and bsa |
| topic | salt-tolerant BSA-seq transcriptomic analysis maize inbred |
| url | https://www.mdpi.com/2223-7747/12/6/1331 |
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