Oxidative stress affects responsiveness to hypotonicity of renal cells
Oxidative stress plays a critical role in the pathophysiology of several kidney diseases and is the consequence of alterations like ischemic events. The regulatory volume decrease (RVD) is an homeostatic response essential to many cells, including renal cells, to counteract changes in the osmolarity...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2014-11-01
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| Series: | Journal of Biological Research |
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| Online Access: | http://www.pagepressjournals.org/index.php/jbr/article/view/4811 |
| _version_ | 1818584961718943744 |
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| author | Rossana Morabito Giuseppa La Spada Silvia Dossena Angela Marino |
| author_facet | Rossana Morabito Giuseppa La Spada Silvia Dossena Angela Marino |
| author_sort | Rossana Morabito |
| collection | DOAJ |
| description | Oxidative stress plays a critical role in the pathophysiology of several kidney diseases and is the consequence of alterations like ischemic events. The regulatory volume decrease (RVD) is an homeostatic response essential to many cells, including renal cells, to counteract changes in the osmolarity of the external medium. The aim of the present work is to verify whether oxidative stress affects RVD in a model of renal cells (human embryonic kidney cells, HEK 293 Phoenix). To accomplish this aim, the experimental procedure consisted in: i) cell culture preparation and treatment with 200 μM H<sub>2</sub>O<sub>2</sub>; and ii) measurement of cell volume changes in isotonic conditions or following hypotonic stress. H<sub>2</sub>O<sub>2</sub> added to the extracellular isotonic solution induced a significant reduction in cell volume, and added to the extracellular hypotonic solution dramatically impaired the expected osmotic cell swelling. Pre-incubation of cells in an extracellular isotonic solution containing H<sub>2</sub>O<sub>2</sub> prevented cell from swelling after hypotonic stress application. In conclusion, H<sub>2</sub>O<sub>2</sub> leads to cell shrinkage in isotonic conditions, inhibits the hypotonicity-induced cell swelling and consequently prevents RVD, hypothetically due to an activation of transport pathways determining ion loss and, in turn, water efflux. Cell shrinkage in isotonic conditions is a hallmark of apoptosis and is known as the apoptotic volume decrease. |
| first_indexed | 2024-12-16T08:29:30Z |
| format | Article |
| id | doaj.art-cb4bfa2f10f240499d2a0abf1f842310 |
| institution | Directory Open Access Journal |
| issn | 1826-8838 2284-0230 |
| language | English |
| last_indexed | 2024-12-16T08:29:30Z |
| publishDate | 2014-11-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Journal of Biological Research |
| spelling | doaj.art-cb4bfa2f10f240499d2a0abf1f8423102022-12-21T22:37:54ZengPAGEPress PublicationsJournal of Biological Research1826-88382284-02302014-11-0187210.4081/jbr.2014.48113969Oxidative stress affects responsiveness to hypotonicity of renal cellsRossana Morabito0Giuseppa La Spada1Silvia Dossena2Angela Marino3Department of Human and Social Sciences, University of MessinaDepartment of Biological and Environmental Sciences, University of MessinaInstitute of Pharmacology and Toxicology, Paracelsus Medizinische Privatuniversität, SalzburgDepartment of Biological and Environmental Sciences, University of MessinaOxidative stress plays a critical role in the pathophysiology of several kidney diseases and is the consequence of alterations like ischemic events. The regulatory volume decrease (RVD) is an homeostatic response essential to many cells, including renal cells, to counteract changes in the osmolarity of the external medium. The aim of the present work is to verify whether oxidative stress affects RVD in a model of renal cells (human embryonic kidney cells, HEK 293 Phoenix). To accomplish this aim, the experimental procedure consisted in: i) cell culture preparation and treatment with 200 μM H<sub>2</sub>O<sub>2</sub>; and ii) measurement of cell volume changes in isotonic conditions or following hypotonic stress. H<sub>2</sub>O<sub>2</sub> added to the extracellular isotonic solution induced a significant reduction in cell volume, and added to the extracellular hypotonic solution dramatically impaired the expected osmotic cell swelling. Pre-incubation of cells in an extracellular isotonic solution containing H<sub>2</sub>O<sub>2</sub> prevented cell from swelling after hypotonic stress application. In conclusion, H<sub>2</sub>O<sub>2</sub> leads to cell shrinkage in isotonic conditions, inhibits the hypotonicity-induced cell swelling and consequently prevents RVD, hypothetically due to an activation of transport pathways determining ion loss and, in turn, water efflux. Cell shrinkage in isotonic conditions is a hallmark of apoptosis and is known as the apoptotic volume decrease.http://www.pagepressjournals.org/index.php/jbr/article/view/4811cell volume regulation, RVD, oxidative stress, kidney cells |
| spellingShingle | Rossana Morabito Giuseppa La Spada Silvia Dossena Angela Marino Oxidative stress affects responsiveness to hypotonicity of renal cells Journal of Biological Research cell volume regulation, RVD, oxidative stress, kidney cells |
| title | Oxidative stress affects responsiveness to hypotonicity of renal cells |
| title_full | Oxidative stress affects responsiveness to hypotonicity of renal cells |
| title_fullStr | Oxidative stress affects responsiveness to hypotonicity of renal cells |
| title_full_unstemmed | Oxidative stress affects responsiveness to hypotonicity of renal cells |
| title_short | Oxidative stress affects responsiveness to hypotonicity of renal cells |
| title_sort | oxidative stress affects responsiveness to hypotonicity of renal cells |
| topic | cell volume regulation, RVD, oxidative stress, kidney cells |
| url | http://www.pagepressjournals.org/index.php/jbr/article/view/4811 |
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