Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template
Tissue engineering and regenerative medicine approaches use biomaterials in combination with cells to regenerate lost functions of tissues and organs to prevent organ transplantation. However, most of the current strategies fail in mimicking the tissue’s extracellular matrix properties. In order to...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/3/232 |
| _version_ | 1797395260193636352 |
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| author | Gerard Rubí-Sans Irene Cano-Torres Soledad Pérez-Amodio Barbara Blanco-Fernandez Miguel A. Mateos-Timoneda Elisabeth Engel |
| author_facet | Gerard Rubí-Sans Irene Cano-Torres Soledad Pérez-Amodio Barbara Blanco-Fernandez Miguel A. Mateos-Timoneda Elisabeth Engel |
| author_sort | Gerard Rubí-Sans |
| collection | DOAJ |
| description | Tissue engineering and regenerative medicine approaches use biomaterials in combination with cells to regenerate lost functions of tissues and organs to prevent organ transplantation. However, most of the current strategies fail in mimicking the tissue’s extracellular matrix properties. In order to mimic native tissue conditions, we developed cell-derived matrix (CDM) microtissues (MT). Our methodology uses poly-lactic acid (PLA) and Cultispher<sup>®</sup> S microcarriers’ (MCs’) as scaffold templates, which are seeded with rat bone marrow mesenchymal stem cells (rBM-MSCs). The scaffold template allows cells to generate an extracellular matrix, which is then extracted for downstream use. The newly formed CDM provides cells with a complex physical (MT architecture) and biochemical (deposited ECM proteins) environment, also showing spontaneous angiogenic potential. Our results suggest that MTs generated from the combination of these two MCs (mixed MTs) are excellent candidates for tissue vascularization. Overall, this study provides a methodology for in-house fabrication of microtissues with angiogenic potential for downstream use in various tissue regenerative strategies. |
| first_indexed | 2024-03-09T00:31:50Z |
| format | Article |
| id | doaj.art-cb51f069809b441283872f180cb89508 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T00:31:50Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-cb51f069809b441283872f180cb895082023-12-11T18:28:36ZengMDPI AGBiomedicines2227-90592021-02-019323210.3390/biomedicines9030232Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-TemplateGerard Rubí-Sans0Irene Cano-Torres1Soledad Pérez-Amodio2Barbara Blanco-Fernandez3Miguel A. Mateos-Timoneda4Elisabeth Engel5Biomaterials for Regenerative Therapies Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, SpainBiomaterials for Regenerative Therapies Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, SpainBiomaterials for Regenerative Therapies Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, SpainBiomaterials for Regenerative Therapies Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, SpainBiomaterials for Regenerative Therapies Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, SpainBiomaterials for Regenerative Therapies Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, SpainTissue engineering and regenerative medicine approaches use biomaterials in combination with cells to regenerate lost functions of tissues and organs to prevent organ transplantation. However, most of the current strategies fail in mimicking the tissue’s extracellular matrix properties. In order to mimic native tissue conditions, we developed cell-derived matrix (CDM) microtissues (MT). Our methodology uses poly-lactic acid (PLA) and Cultispher<sup>®</sup> S microcarriers’ (MCs’) as scaffold templates, which are seeded with rat bone marrow mesenchymal stem cells (rBM-MSCs). The scaffold template allows cells to generate an extracellular matrix, which is then extracted for downstream use. The newly formed CDM provides cells with a complex physical (MT architecture) and biochemical (deposited ECM proteins) environment, also showing spontaneous angiogenic potential. Our results suggest that MTs generated from the combination of these two MCs (mixed MTs) are excellent candidates for tissue vascularization. Overall, this study provides a methodology for in-house fabrication of microtissues with angiogenic potential for downstream use in various tissue regenerative strategies.https://www.mdpi.com/2227-9059/9/3/232poly-lactic acid microcarriersCultispher<sup>®</sup> Srat bone marrow mesenchymal stem cellsmicrotissuecell-derived matrixangiogenesis |
| spellingShingle | Gerard Rubí-Sans Irene Cano-Torres Soledad Pérez-Amodio Barbara Blanco-Fernandez Miguel A. Mateos-Timoneda Elisabeth Engel Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template Biomedicines poly-lactic acid microcarriers Cultispher<sup>®</sup> S rat bone marrow mesenchymal stem cells microtissue cell-derived matrix angiogenesis |
| title | Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template |
| title_full | Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template |
| title_fullStr | Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template |
| title_full_unstemmed | Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template |
| title_short | Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template |
| title_sort | development and angiogenic potential of cell derived microtissues using microcarrier template |
| topic | poly-lactic acid microcarriers Cultispher<sup>®</sup> S rat bone marrow mesenchymal stem cells microtissue cell-derived matrix angiogenesis |
| url | https://www.mdpi.com/2227-9059/9/3/232 |
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