Constitutive ALS3 expression in Candida albicans enhances adhesion and biofilm formation of efg1, but not cph1 mutant strains.
Adhesion to living and non-living surfaces is an important virulence trait of the fungal pathogen Candida albicans. Biofilm formation in this organism depends on the expression of a number of cell surface proteins including the hypha-specific protein Als3p. Loss of ALS3 impairs biofilm formation and...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0286547&type=printable |
| _version_ | 1797394505893150720 |
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| author | Nicholas C Schena Kassandra M Baker Anna A Stark Derek P Thomas Ian A Cleary |
| author_facet | Nicholas C Schena Kassandra M Baker Anna A Stark Derek P Thomas Ian A Cleary |
| author_sort | Nicholas C Schena |
| collection | DOAJ |
| description | Adhesion to living and non-living surfaces is an important virulence trait of the fungal pathogen Candida albicans. Biofilm formation in this organism depends on the expression of a number of cell surface proteins including the hypha-specific protein Als3p. Loss of ALS3 impairs biofilm formation and decreases cell-cell adhesion. We wanted to test whether constitutively expressing ALS3 could compensate for defects in adhesion and biofilm formation observed in mutant strains that lack key transcriptional regulators of biofilm formation Efg1p and Cph1p. We found that ALS3 improved adhesion and biofilm formation in the efg1Δ and efg1Δ cph1Δ mutant strains, but had less effect on the cph1Δ strain. |
| first_indexed | 2024-03-09T00:20:46Z |
| format | Article |
| id | doaj.art-cb5464db54ae435da948988c384a74d3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-09T00:20:46Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cb5464db54ae435da948988c384a74d32023-12-12T05:35:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01187e028654710.1371/journal.pone.0286547Constitutive ALS3 expression in Candida albicans enhances adhesion and biofilm formation of efg1, but not cph1 mutant strains.Nicholas C SchenaKassandra M BakerAnna A StarkDerek P ThomasIan A ClearyAdhesion to living and non-living surfaces is an important virulence trait of the fungal pathogen Candida albicans. Biofilm formation in this organism depends on the expression of a number of cell surface proteins including the hypha-specific protein Als3p. Loss of ALS3 impairs biofilm formation and decreases cell-cell adhesion. We wanted to test whether constitutively expressing ALS3 could compensate for defects in adhesion and biofilm formation observed in mutant strains that lack key transcriptional regulators of biofilm formation Efg1p and Cph1p. We found that ALS3 improved adhesion and biofilm formation in the efg1Δ and efg1Δ cph1Δ mutant strains, but had less effect on the cph1Δ strain.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0286547&type=printable |
| spellingShingle | Nicholas C Schena Kassandra M Baker Anna A Stark Derek P Thomas Ian A Cleary Constitutive ALS3 expression in Candida albicans enhances adhesion and biofilm formation of efg1, but not cph1 mutant strains. PLoS ONE |
| title | Constitutive ALS3 expression in Candida albicans enhances adhesion and biofilm formation of efg1, but not cph1 mutant strains. |
| title_full | Constitutive ALS3 expression in Candida albicans enhances adhesion and biofilm formation of efg1, but not cph1 mutant strains. |
| title_fullStr | Constitutive ALS3 expression in Candida albicans enhances adhesion and biofilm formation of efg1, but not cph1 mutant strains. |
| title_full_unstemmed | Constitutive ALS3 expression in Candida albicans enhances adhesion and biofilm formation of efg1, but not cph1 mutant strains. |
| title_short | Constitutive ALS3 expression in Candida albicans enhances adhesion and biofilm formation of efg1, but not cph1 mutant strains. |
| title_sort | constitutive als3 expression in candida albicans enhances adhesion and biofilm formation of efg1 but not cph1 mutant strains |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0286547&type=printable |
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