Evaluation of Osteogenic Potential for Rat Adipose-Derived Stem Cells under Xeno-Free Environment
This study aimed to develop a novel culture method for rat adipose-derived stem cells (rADSC) and evaluate their osteogenic potential. The rADSC cultured in xeno-free culture medium (XF-rADSCs) or conventional culture medium containing fetal bovine serum (FBS-rADSCs) were combined with micropieces o...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/24/17532 |
| _version_ | 1797380723380846592 |
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| author | Yuzhu Sun Jun-Ichiro Jo Yoshiya Hashimoto |
| author_facet | Yuzhu Sun Jun-Ichiro Jo Yoshiya Hashimoto |
| author_sort | Yuzhu Sun |
| collection | DOAJ |
| description | This study aimed to develop a novel culture method for rat adipose-derived stem cells (rADSC) and evaluate their osteogenic potential. The rADSC cultured in xeno-free culture medium (XF-rADSCs) or conventional culture medium containing fetal bovine serum (FBS-rADSCs) were combined with micropieces of xeno-free recombinant collagen peptide to form 3-dimensional aggregates (XF-rADSC-CellSaic or FBS-rADSC-CellSaic). Both FBS-rADSC and XF-ADSC in CellSaic exhibited multilineage differentiation potential. Compared to FBS-rADSC-CellSaic, XF-rADSC-CellSaic accelerated and promoted osteogenic differentiation in vitro. When transplanted into rat mandibular congenital bone defects, the osteogenically differentiated XF-rADSC-CellSaic induced regeneration of bone tissue with a highly maturated structure compared to FBS-rADSC-CellSaic. In conclusion, XF-rADSC-CellSaic is a feasible 3-dimensional platform for efficient bone formation. |
| first_indexed | 2024-03-08T20:41:12Z |
| format | Article |
| id | doaj.art-cb56224413c24423a41edf69a2b99d9e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T20:41:12Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-cb56224413c24423a41edf69a2b99d9e2023-12-22T14:15:00ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0124241753210.3390/ijms242417532Evaluation of Osteogenic Potential for Rat Adipose-Derived Stem Cells under Xeno-Free EnvironmentYuzhu Sun0Jun-Ichiro Jo1Yoshiya Hashimoto2Department of Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata 573-1121, Osaka, JapanDepartment of Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata 573-1121, Osaka, JapanDepartment of Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata 573-1121, Osaka, JapanThis study aimed to develop a novel culture method for rat adipose-derived stem cells (rADSC) and evaluate their osteogenic potential. The rADSC cultured in xeno-free culture medium (XF-rADSCs) or conventional culture medium containing fetal bovine serum (FBS-rADSCs) were combined with micropieces of xeno-free recombinant collagen peptide to form 3-dimensional aggregates (XF-rADSC-CellSaic or FBS-rADSC-CellSaic). Both FBS-rADSC and XF-ADSC in CellSaic exhibited multilineage differentiation potential. Compared to FBS-rADSC-CellSaic, XF-rADSC-CellSaic accelerated and promoted osteogenic differentiation in vitro. When transplanted into rat mandibular congenital bone defects, the osteogenically differentiated XF-rADSC-CellSaic induced regeneration of bone tissue with a highly maturated structure compared to FBS-rADSC-CellSaic. In conclusion, XF-rADSC-CellSaic is a feasible 3-dimensional platform for efficient bone formation.https://www.mdpi.com/1422-0067/24/24/17532three-dimensional scaffoldrat adipose-derived stem cells (rADSC)xeno-free cultureosteogenic differentiationrat congenital cleft-jaw modelbone repair |
| spellingShingle | Yuzhu Sun Jun-Ichiro Jo Yoshiya Hashimoto Evaluation of Osteogenic Potential for Rat Adipose-Derived Stem Cells under Xeno-Free Environment International Journal of Molecular Sciences three-dimensional scaffold rat adipose-derived stem cells (rADSC) xeno-free culture osteogenic differentiation rat congenital cleft-jaw model bone repair |
| title | Evaluation of Osteogenic Potential for Rat Adipose-Derived Stem Cells under Xeno-Free Environment |
| title_full | Evaluation of Osteogenic Potential for Rat Adipose-Derived Stem Cells under Xeno-Free Environment |
| title_fullStr | Evaluation of Osteogenic Potential for Rat Adipose-Derived Stem Cells under Xeno-Free Environment |
| title_full_unstemmed | Evaluation of Osteogenic Potential for Rat Adipose-Derived Stem Cells under Xeno-Free Environment |
| title_short | Evaluation of Osteogenic Potential for Rat Adipose-Derived Stem Cells under Xeno-Free Environment |
| title_sort | evaluation of osteogenic potential for rat adipose derived stem cells under xeno free environment |
| topic | three-dimensional scaffold rat adipose-derived stem cells (rADSC) xeno-free culture osteogenic differentiation rat congenital cleft-jaw model bone repair |
| url | https://www.mdpi.com/1422-0067/24/24/17532 |
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