Human umbilical cord-derived mesenchymal stem cells protect against experimental colitis via CD5+ B regulatory cells
Abstract Background To clarify the effect of human umbilical cord-derived mesenchymal stem cell (hUC-MSCs) treatment on colitis and to explore the role of CD5+ B cells in MSC therapy. Methods The trinitrobenzenesulfonic acid (TNBS)-induced colitis mouse model was used. HUC-MSCs were transferred peri...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2016-08-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13287-016-0376-2 |
| _version_ | 1811267337708371968 |
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| author | Kang Chao Shenghong Zhang Yun Qiu Xiaoyong Chen Xiaoran Zhang Chuang Cai Yanwen Peng Ren Mao Meirav Pevsner-Fischer Shomron Ben-horin Eran Elinav Zhirong Zeng Baili Chen Yao He Andy Peng Xiang Minhu Chen |
| author_facet | Kang Chao Shenghong Zhang Yun Qiu Xiaoyong Chen Xiaoran Zhang Chuang Cai Yanwen Peng Ren Mao Meirav Pevsner-Fischer Shomron Ben-horin Eran Elinav Zhirong Zeng Baili Chen Yao He Andy Peng Xiang Minhu Chen |
| author_sort | Kang Chao |
| collection | DOAJ |
| description | Abstract Background To clarify the effect of human umbilical cord-derived mesenchymal stem cell (hUC-MSCs) treatment on colitis and to explore the role of CD5+ B cells in MSC therapy. Methods The trinitrobenzenesulfonic acid (TNBS)-induced colitis mouse model was used. HUC-MSCs were transferred peritoneally. Survival rates, colitis symptoms, and macroscopic and histologic scores were evaluated. CD4+ T helper (Th) cell subgroups and CD5+ regulatory B cell (Bregs) in lymphocytes were quantitated by flow cytometry. Cytokine levels were detected by ELISA and Bio-plex. CD5+ B cells were isolated for in vitro co-culture and adaptive transfer. Results HUC-MSC treatment alleviated TNBS-induced colitis by increasing survival rates, relieving symptoms, and improving macroscopic and histologic scores. Labeled hUC-MSCs were located in the inflamed areas of colitis mice. Increases in regulatory T cells (Tregs) and CD5+ B cells and decreases in Th1 cells, Th17 cells, and several pro-inflammatory cytokines were observed with hUC-MSC treatment. After adaptive transfer, CD5+ B cells, which were located mainly in the peritoneal lavage fluid, improved TNBS-induced colitis by correcting Treg/Th1/Th17 imbalances. CD5+ B cells also inhibited T-cell proliferation and produced interleukin (IL)-10. Conclusions HUC-MSCs protected against experimental colitis by boosting the numbers of CD5+ B cells and IL-10-producing CD5+ Bregs, and correcting Treg/Th17/Th1 imbalances. |
| first_indexed | 2024-04-12T21:00:48Z |
| format | Article |
| id | doaj.art-cb5814e152fd4b44af79fba27a72780f |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-04-12T21:00:48Z |
| publishDate | 2016-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-cb5814e152fd4b44af79fba27a72780f2022-12-22T03:16:51ZengBMCStem Cell Research & Therapy1757-65122016-08-017111210.1186/s13287-016-0376-2Human umbilical cord-derived mesenchymal stem cells protect against experimental colitis via CD5+ B regulatory cellsKang Chao0Shenghong Zhang1Yun Qiu2Xiaoyong Chen3Xiaoran Zhang4Chuang Cai5Yanwen Peng6Ren Mao7Meirav Pevsner-Fischer8Shomron Ben-horin9Eran Elinav10Zhirong Zeng11Baili Chen12Yao He13Andy Peng Xiang14Minhu Chen15Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen UniversityDivision of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen UniversityDivision of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen UniversityCenter for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen UniversityCenter for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen UniversityCenter for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen UniversityCenter for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen UniversityDivision of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen UniversityDepartment of Immunology, Weizmann Institute of ScienceDivision of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen UniversityDepartment of Immunology, Weizmann Institute of ScienceDivision of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen UniversityDivision of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen UniversityDivision of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen UniversityCenter for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen UniversityDivision of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen UniversityAbstract Background To clarify the effect of human umbilical cord-derived mesenchymal stem cell (hUC-MSCs) treatment on colitis and to explore the role of CD5+ B cells in MSC therapy. Methods The trinitrobenzenesulfonic acid (TNBS)-induced colitis mouse model was used. HUC-MSCs were transferred peritoneally. Survival rates, colitis symptoms, and macroscopic and histologic scores were evaluated. CD4+ T helper (Th) cell subgroups and CD5+ regulatory B cell (Bregs) in lymphocytes were quantitated by flow cytometry. Cytokine levels were detected by ELISA and Bio-plex. CD5+ B cells were isolated for in vitro co-culture and adaptive transfer. Results HUC-MSC treatment alleviated TNBS-induced colitis by increasing survival rates, relieving symptoms, and improving macroscopic and histologic scores. Labeled hUC-MSCs were located in the inflamed areas of colitis mice. Increases in regulatory T cells (Tregs) and CD5+ B cells and decreases in Th1 cells, Th17 cells, and several pro-inflammatory cytokines were observed with hUC-MSC treatment. After adaptive transfer, CD5+ B cells, which were located mainly in the peritoneal lavage fluid, improved TNBS-induced colitis by correcting Treg/Th1/Th17 imbalances. CD5+ B cells also inhibited T-cell proliferation and produced interleukin (IL)-10. Conclusions HUC-MSCs protected against experimental colitis by boosting the numbers of CD5+ B cells and IL-10-producing CD5+ Bregs, and correcting Treg/Th17/Th1 imbalances.http://link.springer.com/article/10.1186/s13287-016-0376-2Mesenchymal stem cellsColitisCrohn’s diseaseB regulatory cellT helper cell |
| spellingShingle | Kang Chao Shenghong Zhang Yun Qiu Xiaoyong Chen Xiaoran Zhang Chuang Cai Yanwen Peng Ren Mao Meirav Pevsner-Fischer Shomron Ben-horin Eran Elinav Zhirong Zeng Baili Chen Yao He Andy Peng Xiang Minhu Chen Human umbilical cord-derived mesenchymal stem cells protect against experimental colitis via CD5+ B regulatory cells Stem Cell Research & Therapy Mesenchymal stem cells Colitis Crohn’s disease B regulatory cell T helper cell |
| title | Human umbilical cord-derived mesenchymal stem cells protect against experimental colitis via CD5+ B regulatory cells |
| title_full | Human umbilical cord-derived mesenchymal stem cells protect against experimental colitis via CD5+ B regulatory cells |
| title_fullStr | Human umbilical cord-derived mesenchymal stem cells protect against experimental colitis via CD5+ B regulatory cells |
| title_full_unstemmed | Human umbilical cord-derived mesenchymal stem cells protect against experimental colitis via CD5+ B regulatory cells |
| title_short | Human umbilical cord-derived mesenchymal stem cells protect against experimental colitis via CD5+ B regulatory cells |
| title_sort | human umbilical cord derived mesenchymal stem cells protect against experimental colitis via cd5 b regulatory cells |
| topic | Mesenchymal stem cells Colitis Crohn’s disease B regulatory cell T helper cell |
| url | http://link.springer.com/article/10.1186/s13287-016-0376-2 |
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