Genome-Wide Approaches to Drosophila Heart Development
The development of the dorsal vessel in Drosophila is one of the first systems in which key mechanisms regulating cardiogenesis have been defined in great detail at the genetic and molecular level. Due to evolutionary conservation, these findings have also provided major inputs into studies of cardi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-05-01
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| Series: | Journal of Cardiovascular Development and Disease |
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| Online Access: | http://www.mdpi.com/2308-3425/3/2/20 |
| _version_ | 1811255931387772928 |
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| author | Manfred Frasch |
| author_facet | Manfred Frasch |
| author_sort | Manfred Frasch |
| collection | DOAJ |
| description | The development of the dorsal vessel in Drosophila is one of the first systems in which key mechanisms regulating cardiogenesis have been defined in great detail at the genetic and molecular level. Due to evolutionary conservation, these findings have also provided major inputs into studies of cardiogenesis in vertebrates. Many of the major components that control Drosophila cardiogenesis were discovered based on candidate gene approaches and their functions were defined by employing the outstanding genetic tools and molecular techniques available in this system. More recently, approaches have been taken that aim to interrogate the entire genome in order to identify novel components and describe genomic features that are pertinent to the regulation of heart development. Apart from classical forward genetic screens, the availability of the thoroughly annotated Drosophila genome sequence made new genome-wide approaches possible, which include the generation of massive numbers of RNA interference (RNAi) reagents that were used in forward genetic screens, as well as studies of the transcriptomes and proteomes of the developing heart under normal and experimentally manipulated conditions. Moreover, genome-wide chromatin immunoprecipitation experiments have been performed with the aim to define the full set of genomic binding sites of the major cardiogenic transcription factors, their relevant target genes, and a more complete picture of the regulatory network that drives cardiogenesis. This review will give an overview on these genome-wide approaches to Drosophila heart development and on computational analyses of the obtained information that ultimately aim to provide a description of this process at the systems level. |
| first_indexed | 2024-04-12T17:32:24Z |
| format | Article |
| id | doaj.art-cb591825f099425ea157763a500911dd |
| institution | Directory Open Access Journal |
| issn | 2308-3425 |
| language | English |
| last_indexed | 2024-04-12T17:32:24Z |
| publishDate | 2016-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Cardiovascular Development and Disease |
| spelling | doaj.art-cb591825f099425ea157763a500911dd2022-12-22T03:23:06ZengMDPI AGJournal of Cardiovascular Development and Disease2308-34252016-05-01322010.3390/jcdd3020020jcdd3020020Genome-Wide Approaches to Drosophila Heart DevelopmentManfred Frasch0Division of Developmental Biology, Department of Biology, Friedrich-Alexander University of Erlangen-Nürnberg, Staudtstr. 5, Erlangen 91058, GermanyThe development of the dorsal vessel in Drosophila is one of the first systems in which key mechanisms regulating cardiogenesis have been defined in great detail at the genetic and molecular level. Due to evolutionary conservation, these findings have also provided major inputs into studies of cardiogenesis in vertebrates. Many of the major components that control Drosophila cardiogenesis were discovered based on candidate gene approaches and their functions were defined by employing the outstanding genetic tools and molecular techniques available in this system. More recently, approaches have been taken that aim to interrogate the entire genome in order to identify novel components and describe genomic features that are pertinent to the regulation of heart development. Apart from classical forward genetic screens, the availability of the thoroughly annotated Drosophila genome sequence made new genome-wide approaches possible, which include the generation of massive numbers of RNA interference (RNAi) reagents that were used in forward genetic screens, as well as studies of the transcriptomes and proteomes of the developing heart under normal and experimentally manipulated conditions. Moreover, genome-wide chromatin immunoprecipitation experiments have been performed with the aim to define the full set of genomic binding sites of the major cardiogenic transcription factors, their relevant target genes, and a more complete picture of the regulatory network that drives cardiogenesis. This review will give an overview on these genome-wide approaches to Drosophila heart development and on computational analyses of the obtained information that ultimately aim to provide a description of this process at the systems level.http://www.mdpi.com/2308-3425/3/2/20cardiogenesisheart developmentcardiogenic transcription factorsgenetic screensgenomicstranscriptomicsChIP-chipChIP-seqmachine learning |
| spellingShingle | Manfred Frasch Genome-Wide Approaches to Drosophila Heart Development Journal of Cardiovascular Development and Disease cardiogenesis heart development cardiogenic transcription factors genetic screens genomics transcriptomics ChIP-chip ChIP-seq machine learning |
| title | Genome-Wide Approaches to Drosophila Heart Development |
| title_full | Genome-Wide Approaches to Drosophila Heart Development |
| title_fullStr | Genome-Wide Approaches to Drosophila Heart Development |
| title_full_unstemmed | Genome-Wide Approaches to Drosophila Heart Development |
| title_short | Genome-Wide Approaches to Drosophila Heart Development |
| title_sort | genome wide approaches to drosophila heart development |
| topic | cardiogenesis heart development cardiogenic transcription factors genetic screens genomics transcriptomics ChIP-chip ChIP-seq machine learning |
| url | http://www.mdpi.com/2308-3425/3/2/20 |
| work_keys_str_mv | AT manfredfrasch genomewideapproachestodrosophilaheartdevelopment |