Genotype profiles of rotavirus strains in children under 5-year-old outpatients with diarrhea in Bandung, West Java, Indonesia
Introduction: Diarrhea is a global leading cause of morbidity and mortality among children under five, with rotaviruses being the most common cause. This study aimed to determine the genotypes of rotavirus in children under 5 years with diarrhea in Bandung, Indonesia. Methods: This cross-sectional s...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Journal of Global Infectious Diseases |
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| Online Access: | http://www.jgid.org/article.asp?issn=0974-777X;year=2022;volume=14;issue=4;spage=142;epage=146;aulast=Prasetyo |
| _version_ | 1811177566265933824 |
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| author | Dwi Prasetyo Yudith Setiati Ermaya Iesje Martiza Sabaroedin Dyah Widhiastuti Novilia Sjafri Bachtiar Cissy Bana Kartasasmita |
| author_facet | Dwi Prasetyo Yudith Setiati Ermaya Iesje Martiza Sabaroedin Dyah Widhiastuti Novilia Sjafri Bachtiar Cissy Bana Kartasasmita |
| author_sort | Dwi Prasetyo |
| collection | DOAJ |
| description | Introduction: Diarrhea is a global leading cause of morbidity and mortality among children under five, with rotaviruses being the most common cause. This study aimed to determine the genotypes of rotavirus in children under 5 years with diarrhea in Bandung, Indonesia. Methods: This cross-sectional study was conducted from 2014 to 2018 on 450 children under five with acute diarrhea in primary health centers in Bandung, Indonesia. Fecal samples were examined for rotavirus antigen using an enzyme-linked immunosorbent assay method, and genotype was determined through sequencing using polymerase chain reaction. Results were statistically analyzed using Pearson Chi-square in Epi Info version 3.5.4, with P < 0.05 considered statistically significant. Results: Rotavirus was identified in 8.9% of the subjects, slightly higher in boys (n = 24, 9.8%) than girls (n = 16, 7.8%). We found that the most rotavirus positive in age group is >12–24 months and >24–59 months, while the highest percentage is at the age of ≤6 months (11.8%). Moderate malnutrition was observed in more subjects (12.8%). Vomiting was more frequent in patients positive (55%, P = 0.013) and fever was seen in 32.5% (P = 0.645). No signs of dehydration were seen in most subjects (75%), P = 0.227. Rotavirus genotypes identified were G1P[8] (18, 45%), G3P[8] (14, 35%), G3P[6] (4, 10%), G3P[9] (2, 5%), G2P[4] (1, 2.5%), and nontypeable (NT) (1, 2.5%). Conclusions: The dominant rotavirus genotype is G1P[8], followed by G3P[8], G3P[6], G3P[9], G2P[4], and NT. The most common rotavirus positive in age group is >12–24 months and >24–59 months, while the highest percentage is at the age of ≤6 months. |
| first_indexed | 2024-04-11T06:03:26Z |
| format | Article |
| id | doaj.art-cb5fbc6dd8e84c6881089e99be16b6fb |
| institution | Directory Open Access Journal |
| issn | 0974-777X |
| language | English |
| last_indexed | 2024-04-11T06:03:26Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Global Infectious Diseases |
| spelling | doaj.art-cb5fbc6dd8e84c6881089e99be16b6fb2022-12-22T04:41:33ZengWolters Kluwer Medknow PublicationsJournal of Global Infectious Diseases0974-777X2022-01-0114414214610.4103/jgid.jgid_101_22Genotype profiles of rotavirus strains in children under 5-year-old outpatients with diarrhea in Bandung, West Java, IndonesiaDwi PrasetyoYudith Setiati ErmayaIesje Martiza SabaroedinDyah WidhiastutiNovilia Sjafri BachtiarCissy Bana KartasasmitaIntroduction: Diarrhea is a global leading cause of morbidity and mortality among children under five, with rotaviruses being the most common cause. This study aimed to determine the genotypes of rotavirus in children under 5 years with diarrhea in Bandung, Indonesia. Methods: This cross-sectional study was conducted from 2014 to 2018 on 450 children under five with acute diarrhea in primary health centers in Bandung, Indonesia. Fecal samples were examined for rotavirus antigen using an enzyme-linked immunosorbent assay method, and genotype was determined through sequencing using polymerase chain reaction. Results were statistically analyzed using Pearson Chi-square in Epi Info version 3.5.4, with P < 0.05 considered statistically significant. Results: Rotavirus was identified in 8.9% of the subjects, slightly higher in boys (n = 24, 9.8%) than girls (n = 16, 7.8%). We found that the most rotavirus positive in age group is >12–24 months and >24–59 months, while the highest percentage is at the age of ≤6 months (11.8%). Moderate malnutrition was observed in more subjects (12.8%). Vomiting was more frequent in patients positive (55%, P = 0.013) and fever was seen in 32.5% (P = 0.645). No signs of dehydration were seen in most subjects (75%), P = 0.227. Rotavirus genotypes identified were G1P[8] (18, 45%), G3P[8] (14, 35%), G3P[6] (4, 10%), G3P[9] (2, 5%), G2P[4] (1, 2.5%), and nontypeable (NT) (1, 2.5%). Conclusions: The dominant rotavirus genotype is G1P[8], followed by G3P[8], G3P[6], G3P[9], G2P[4], and NT. The most common rotavirus positive in age group is >12–24 months and >24–59 months, while the highest percentage is at the age of ≤6 months.http://www.jgid.org/article.asp?issn=0974-777X;year=2022;volume=14;issue=4;spage=142;epage=146;aulast=Prasetyochildrengenotypeoutpatientrotavirus |
| spellingShingle | Dwi Prasetyo Yudith Setiati Ermaya Iesje Martiza Sabaroedin Dyah Widhiastuti Novilia Sjafri Bachtiar Cissy Bana Kartasasmita Genotype profiles of rotavirus strains in children under 5-year-old outpatients with diarrhea in Bandung, West Java, Indonesia Journal of Global Infectious Diseases children genotype outpatient rotavirus |
| title | Genotype profiles of rotavirus strains in children under 5-year-old outpatients with diarrhea in Bandung, West Java, Indonesia |
| title_full | Genotype profiles of rotavirus strains in children under 5-year-old outpatients with diarrhea in Bandung, West Java, Indonesia |
| title_fullStr | Genotype profiles of rotavirus strains in children under 5-year-old outpatients with diarrhea in Bandung, West Java, Indonesia |
| title_full_unstemmed | Genotype profiles of rotavirus strains in children under 5-year-old outpatients with diarrhea in Bandung, West Java, Indonesia |
| title_short | Genotype profiles of rotavirus strains in children under 5-year-old outpatients with diarrhea in Bandung, West Java, Indonesia |
| title_sort | genotype profiles of rotavirus strains in children under 5 year old outpatients with diarrhea in bandung west java indonesia |
| topic | children genotype outpatient rotavirus |
| url | http://www.jgid.org/article.asp?issn=0974-777X;year=2022;volume=14;issue=4;spage=142;epage=146;aulast=Prasetyo |
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