Dietary n-3 polyunsaturated fat increases the fractional catabolic rate of medium-sized HDL particles in African green monkeys
We have previously described a novel pathway for the metabolism of HDL subfractions in which small [2 apolipoprotein (apoA-I) molecules per particle] HDL particles are converted in a unidirectional manner outside the plasma compartment to medium (3 apoA-I molecules per particle) or large (4 apoA-I m...
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| Format: | Article |
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Elsevier
2001-09-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520302790 |
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| author | Kevin W. Huggins Perry L. Colvin Ellen R. Burleson Kathryn Kelley Janet K. Sawyer P. Hugh R. Barrett Lawrence L. Rudel John S. Parks |
| author_facet | Kevin W. Huggins Perry L. Colvin Ellen R. Burleson Kathryn Kelley Janet K. Sawyer P. Hugh R. Barrett Lawrence L. Rudel John S. Parks |
| author_sort | Kevin W. Huggins |
| collection | DOAJ |
| description | We have previously described a novel pathway for the metabolism of HDL subfractions in which small [2 apolipoprotein (apoA-I) molecules per particle] HDL particles are converted in a unidirectional manner outside the plasma compartment to medium (3 apoA-I molecules per particle) or large (4 apoA-I molecules per particle) HDL particles, which are subsequently removed from the circulation by the liver (Colvin et al. 1999. J. Lipid Res. 40: 1782–1792; Huggins et al. 2000. J. Lipid Res. 41: 384–394). The purpose of the present study was to determine whether the reduction in concentration of medium HDL in African green monkeys consuming n-3 polyunsaturated versus saturated fat diets resulted from decreased in vivo production or increased catabolism. Tracer small LpA-I (HDL containing only apoA-I) were isolated, without ultracentrifugation, by gel filtration and immunoaffinity chromatography and radiolabeled. After injection, the specific activity of apoA-I in small, medium, and large HDL was determined, and the kinetic data were analyzed using our previously published multicompartmental model for HDL subfraction metabolism. We found a significant reduction of apoA-I concentration in medium HDL in the animals fed n-3 polyunsaturated fat (31.2 ± 0.7 mg/dl) compared with animals fed saturated fat (85.4 ± 11.9 mg/dl; P = 0.002). The production rates of apoA-I in small, medium, and large HDL were similar in both diet groups; however, there was a significant increase in the fractional catabolic rate of apoA-I in medium HDL in the animals fed n-3 polyunsaturated fat (2.188 ± 0.501 pools/day) compared with animals fed saturated fat (0.714 ± 0.191 pools/day; P = 0.02). We conclude that n-3 polyunsaturated fat reduces HDL cholesterol concentration by increasing the fractional catabolic rate of medium-sized HDL particles in African green monkeys. —Huggins, K. W., P. L. Colvin, E. R. Burleson, K. Kelley, J. K. Sawyer, P. H. R. Barrett, L. L. Rudel, and J. S. Parks. Dietary n-3 polyunsaturated fat increases the fractional catabolic rate of medium-sized HDL particles in African green monkeys. |
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| language | English |
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| publishDate | 2001-09-01 |
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| spelling | doaj.art-cb603f16b7d64d72b5c5477457ceca9a2022-12-21T18:53:50ZengElsevierJournal of Lipid Research0022-22752001-09-0142914571466Dietary n-3 polyunsaturated fat increases the fractional catabolic rate of medium-sized HDL particles in African green monkeysKevin W. Huggins0Perry L. Colvin1Ellen R. Burleson2Kathryn Kelley3Janet K. Sawyer4P. Hugh R. Barrett5Lawrence L. Rudel6John S. Parks7Department of Pathology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157Department of Internal Medicine and Division of Gerontology, University of Maryland School of Medicine and the Baltimore Veterans Affairs Medical Center, Geriatrics Research, Education, and Clinical Center, Baltimore, MD 21201Department of Pathology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157Department of Pathology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157Department of Pathology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157Department of Medicine, University of Western Australia, Box X2213 GPO, Perth, Australia 6847Department of Pathology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157To whom correspondence should be addressed. e-mail:; Department of Pathology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157We have previously described a novel pathway for the metabolism of HDL subfractions in which small [2 apolipoprotein (apoA-I) molecules per particle] HDL particles are converted in a unidirectional manner outside the plasma compartment to medium (3 apoA-I molecules per particle) or large (4 apoA-I molecules per particle) HDL particles, which are subsequently removed from the circulation by the liver (Colvin et al. 1999. J. Lipid Res. 40: 1782–1792; Huggins et al. 2000. J. Lipid Res. 41: 384–394). The purpose of the present study was to determine whether the reduction in concentration of medium HDL in African green monkeys consuming n-3 polyunsaturated versus saturated fat diets resulted from decreased in vivo production or increased catabolism. Tracer small LpA-I (HDL containing only apoA-I) were isolated, without ultracentrifugation, by gel filtration and immunoaffinity chromatography and radiolabeled. After injection, the specific activity of apoA-I in small, medium, and large HDL was determined, and the kinetic data were analyzed using our previously published multicompartmental model for HDL subfraction metabolism. We found a significant reduction of apoA-I concentration in medium HDL in the animals fed n-3 polyunsaturated fat (31.2 ± 0.7 mg/dl) compared with animals fed saturated fat (85.4 ± 11.9 mg/dl; P = 0.002). The production rates of apoA-I in small, medium, and large HDL were similar in both diet groups; however, there was a significant increase in the fractional catabolic rate of apoA-I in medium HDL in the animals fed n-3 polyunsaturated fat (2.188 ± 0.501 pools/day) compared with animals fed saturated fat (0.714 ± 0.191 pools/day; P = 0.02). We conclude that n-3 polyunsaturated fat reduces HDL cholesterol concentration by increasing the fractional catabolic rate of medium-sized HDL particles in African green monkeys. —Huggins, K. W., P. L. Colvin, E. R. Burleson, K. Kelley, J. K. Sawyer, P. H. R. Barrett, L. L. Rudel, and J. S. Parks. Dietary n-3 polyunsaturated fat increases the fractional catabolic rate of medium-sized HDL particles in African green monkeys.http://www.sciencedirect.com/science/article/pii/S0022227520302790fish oilLpA-Iapolipoprotein A-IPUFAnon-human primatessaturated fat |
| spellingShingle | Kevin W. Huggins Perry L. Colvin Ellen R. Burleson Kathryn Kelley Janet K. Sawyer P. Hugh R. Barrett Lawrence L. Rudel John S. Parks Dietary n-3 polyunsaturated fat increases the fractional catabolic rate of medium-sized HDL particles in African green monkeys Journal of Lipid Research fish oil LpA-I apolipoprotein A-I PUFA non-human primates saturated fat |
| title | Dietary n-3 polyunsaturated fat increases the fractional catabolic rate of medium-sized HDL particles in African green monkeys |
| title_full | Dietary n-3 polyunsaturated fat increases the fractional catabolic rate of medium-sized HDL particles in African green monkeys |
| title_fullStr | Dietary n-3 polyunsaturated fat increases the fractional catabolic rate of medium-sized HDL particles in African green monkeys |
| title_full_unstemmed | Dietary n-3 polyunsaturated fat increases the fractional catabolic rate of medium-sized HDL particles in African green monkeys |
| title_short | Dietary n-3 polyunsaturated fat increases the fractional catabolic rate of medium-sized HDL particles in African green monkeys |
| title_sort | dietary n 3 polyunsaturated fat increases the fractional catabolic rate of medium sized hdl particles in african green monkeys |
| topic | fish oil LpA-I apolipoprotein A-I PUFA non-human primates saturated fat |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520302790 |
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