Pituitary Actions of EGF on Gonadotropins, Growth Hormone, Prolactin and Somatolactins in Grass Carp
In mammals, epidermal growth factor (EGF) plays a vital role in both pituitary physiology and pathology. However, the functional role of EGF in the regulation of pituitary hormones has rarely reported in teleost. In our study, using primary cultured grass carp pituitary cells as an in vitro model, w...
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MDPI AG
2020-09-01
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| Online Access: | https://www.mdpi.com/2079-7737/9/9/279 |
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| author | Qiongyao Hu Qinbo Qin Shaohua Xu Lingling Zhou Chuanhui Xia Xuetao Shi Huiying Zhang Jingyi Jia Cheng Ye Zhan Yin Guangfu Hu |
| author_facet | Qiongyao Hu Qinbo Qin Shaohua Xu Lingling Zhou Chuanhui Xia Xuetao Shi Huiying Zhang Jingyi Jia Cheng Ye Zhan Yin Guangfu Hu |
| author_sort | Qiongyao Hu |
| collection | DOAJ |
| description | In mammals, epidermal growth factor (EGF) plays a vital role in both pituitary physiology and pathology. However, the functional role of EGF in the regulation of pituitary hormones has rarely reported in teleost. In our study, using primary cultured grass carp pituitary cells as an in vitro model, we examined the effects of EGF on pituitary hormone secretion and gene expression as well as the post-receptor signaling mechanisms involved. Firstly, we found that EGF significantly reduced luteinizing hormone (LHβ) mRNA expression via ErbB1 coupled to ERK1/2 pathway, but had no effect on LH release in grass carp pituitary cells. Secondly, the results showed that EGF was effective in up-regulating mRNA expression of growth hormone (GH), somatolactin α (SLα) and somatolactin β (SLβ) via ErbB1 and ErbB2 and subsequently coupled to MEK1/2/ERK1/2 and PI3K/Akt/mTOR pathways, respectively. However, EGF was not effective in GH release in pituitary cells. Thirdly, we found that EGF strongly induced pituitary prolactin (PRL) release and mRNA expression, which was mediated by ErbB1 and subsequent stimulation of MEK1/2/ERK1/2 and PI3K/Akt/mTOR pathways. Interestingly, subsequent study further found that neurokinin B (NKB) significantly suppressed EGF-induced PRL mRNA expression, which was mediated by neurokinin receptor (NK2R) and coupled to AC/cAMP/PKA signal pathway. These results suggested that EGF could differently regulate the pituitary hormones expression in grass carp pituitary cells. |
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| last_indexed | 2024-03-10T16:30:02Z |
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| spelling | doaj.art-cb6156d982be497f9560a81b59a19bcc2023-11-20T12:56:12ZengMDPI AGBiology2079-77372020-09-019927910.3390/biology9090279Pituitary Actions of EGF on Gonadotropins, Growth Hormone, Prolactin and Somatolactins in Grass CarpQiongyao Hu0Qinbo Qin1Shaohua Xu2Lingling Zhou3Chuanhui Xia4Xuetao Shi5Huiying Zhang6Jingyi Jia7Cheng Ye8Zhan Yin9Guangfu Hu10College of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha 410081, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan 430070, ChinaIn mammals, epidermal growth factor (EGF) plays a vital role in both pituitary physiology and pathology. However, the functional role of EGF in the regulation of pituitary hormones has rarely reported in teleost. In our study, using primary cultured grass carp pituitary cells as an in vitro model, we examined the effects of EGF on pituitary hormone secretion and gene expression as well as the post-receptor signaling mechanisms involved. Firstly, we found that EGF significantly reduced luteinizing hormone (LHβ) mRNA expression via ErbB1 coupled to ERK1/2 pathway, but had no effect on LH release in grass carp pituitary cells. Secondly, the results showed that EGF was effective in up-regulating mRNA expression of growth hormone (GH), somatolactin α (SLα) and somatolactin β (SLβ) via ErbB1 and ErbB2 and subsequently coupled to MEK1/2/ERK1/2 and PI3K/Akt/mTOR pathways, respectively. However, EGF was not effective in GH release in pituitary cells. Thirdly, we found that EGF strongly induced pituitary prolactin (PRL) release and mRNA expression, which was mediated by ErbB1 and subsequent stimulation of MEK1/2/ERK1/2 and PI3K/Akt/mTOR pathways. Interestingly, subsequent study further found that neurokinin B (NKB) significantly suppressed EGF-induced PRL mRNA expression, which was mediated by neurokinin receptor (NK2R) and coupled to AC/cAMP/PKA signal pathway. These results suggested that EGF could differently regulate the pituitary hormones expression in grass carp pituitary cells.https://www.mdpi.com/2079-7737/9/9/279EGFErbB1prolactingrowth hormoneLHβNKB |
| spellingShingle | Qiongyao Hu Qinbo Qin Shaohua Xu Lingling Zhou Chuanhui Xia Xuetao Shi Huiying Zhang Jingyi Jia Cheng Ye Zhan Yin Guangfu Hu Pituitary Actions of EGF on Gonadotropins, Growth Hormone, Prolactin and Somatolactins in Grass Carp Biology EGF ErbB1 prolactin growth hormone LHβ NKB |
| title | Pituitary Actions of EGF on Gonadotropins, Growth Hormone, Prolactin and Somatolactins in Grass Carp |
| title_full | Pituitary Actions of EGF on Gonadotropins, Growth Hormone, Prolactin and Somatolactins in Grass Carp |
| title_fullStr | Pituitary Actions of EGF on Gonadotropins, Growth Hormone, Prolactin and Somatolactins in Grass Carp |
| title_full_unstemmed | Pituitary Actions of EGF on Gonadotropins, Growth Hormone, Prolactin and Somatolactins in Grass Carp |
| title_short | Pituitary Actions of EGF on Gonadotropins, Growth Hormone, Prolactin and Somatolactins in Grass Carp |
| title_sort | pituitary actions of egf on gonadotropins growth hormone prolactin and somatolactins in grass carp |
| topic | EGF ErbB1 prolactin growth hormone LHβ NKB |
| url | https://www.mdpi.com/2079-7737/9/9/279 |
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