Purification of Poly (3-hydroxybutyrate) Produced by Fatty Acid Fermentation Using Organic Polar Solvents

Poly (3-hydroxybutyrate) or (P3HB) produced by the fermentation of fatty acids with the mutant strain Burkholderia cepacia B27 was extracted and purified using different techniques including washing and precipitation of the biomass, and the polymeric materials with organic polar solvents such as eth...

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Main Authors: Andres Ramos, Armando Espinosa, Daniel Mendez, Ivan Cabeza, Nubia Moreno - Sarmiento
Format: Article
Language:English
Published: AIDIC Servizi S.r.l. 2018-09-01
Series:Chemical Engineering Transactions
Online Access:https://www.cetjournal.it/index.php/cet/article/view/8998
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author Andres Ramos
Armando Espinosa
Daniel Mendez
Ivan Cabeza
Nubia Moreno - Sarmiento
author_facet Andres Ramos
Armando Espinosa
Daniel Mendez
Ivan Cabeza
Nubia Moreno - Sarmiento
author_sort Andres Ramos
collection DOAJ
description Poly (3-hydroxybutyrate) or (P3HB) produced by the fermentation of fatty acids with the mutant strain Burkholderia cepacia B27 was extracted and purified using different techniques including washing and precipitation of the biomass, and the polymeric materials with organic polar solvents such as ethanol (96%) or methanol (99,7%). The materials obtained after the purification processes were thermally characterized (using differential scanning calorimetry and thermo gravimetric analysis). The polymeric samples treated with organic polar solvents showed improved melting point passing from 169,2°C; for the chloroform purified samples to 174,9°C for the methanol purified samples, also, appearance and odor of the films was improved.
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spelling doaj.art-cb61fac7f908442e9d1ccbf298fe54a72022-12-21T19:43:14ZengAIDIC Servizi S.r.l.Chemical Engineering Transactions2283-92162018-09-016710.3303/CET1867112Purification of Poly (3-hydroxybutyrate) Produced by Fatty Acid Fermentation Using Organic Polar SolventsAndres RamosArmando EspinosaDaniel MendezIvan CabezaNubia Moreno - SarmientoPoly (3-hydroxybutyrate) or (P3HB) produced by the fermentation of fatty acids with the mutant strain Burkholderia cepacia B27 was extracted and purified using different techniques including washing and precipitation of the biomass, and the polymeric materials with organic polar solvents such as ethanol (96%) or methanol (99,7%). The materials obtained after the purification processes were thermally characterized (using differential scanning calorimetry and thermo gravimetric analysis). The polymeric samples treated with organic polar solvents showed improved melting point passing from 169,2°C; for the chloroform purified samples to 174,9°C for the methanol purified samples, also, appearance and odor of the films was improved.https://www.cetjournal.it/index.php/cet/article/view/8998
spellingShingle Andres Ramos
Armando Espinosa
Daniel Mendez
Ivan Cabeza
Nubia Moreno - Sarmiento
Purification of Poly (3-hydroxybutyrate) Produced by Fatty Acid Fermentation Using Organic Polar Solvents
Chemical Engineering Transactions
title Purification of Poly (3-hydroxybutyrate) Produced by Fatty Acid Fermentation Using Organic Polar Solvents
title_full Purification of Poly (3-hydroxybutyrate) Produced by Fatty Acid Fermentation Using Organic Polar Solvents
title_fullStr Purification of Poly (3-hydroxybutyrate) Produced by Fatty Acid Fermentation Using Organic Polar Solvents
title_full_unstemmed Purification of Poly (3-hydroxybutyrate) Produced by Fatty Acid Fermentation Using Organic Polar Solvents
title_short Purification of Poly (3-hydroxybutyrate) Produced by Fatty Acid Fermentation Using Organic Polar Solvents
title_sort purification of poly 3 hydroxybutyrate produced by fatty acid fermentation using organic polar solvents
url https://www.cetjournal.it/index.php/cet/article/view/8998
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