Diferentefects of pulsed X-rays in MOLT-4 cel line and human peripheral blod lymphocytes
Effects of ionizing radiation registered in cells after low dose irradiation are still poorly understood. Thus, the aim of this study was to analyze effects of pulsed X-rays on level of radiation-induced DNA double-strand breaks and their repair kinetics in cancer and normalhuman cells in vitro. Ana...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Russian |
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Russian Academy of Sciences, Tomsk National Research Medical Center
2016-02-01
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| Series: | Сибирский онкологический журнал |
| Subjects: | |
| Online Access: | https://www.siboncoj.ru/jour/article/view/94 |
| _version_ | 1797875960383537152 |
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| author | S. A. Vasilyev A. A. Belenko O. P. Kutenkov M. A. Bolshakov I. N. Lebedev V. V. Rostov |
| author_facet | S. A. Vasilyev A. A. Belenko O. P. Kutenkov M. A. Bolshakov I. N. Lebedev V. V. Rostov |
| author_sort | S. A. Vasilyev |
| collection | DOAJ |
| description | Effects of ionizing radiation registered in cells after low dose irradiation are still poorly understood. Thus, the aim of this study was to analyze effects of pulsed X-rays on level of radiation-induced DNA double-strand breaks and their repair kinetics in cancer and normalhuman cells in vitro. Analysis of radiation-induced γH2AX and 53BP1 repair foci in MOLT-4 cells with lymphoblastic origin was used for assessment of DNA double-strand breaks (DSB) in these cells. Number of residual radiation-induced γH2AX and 53BP1 foci at 18 hafter irradiation depended on frequency of X-ray pulses: at 8 pulses per second effect was highest in MOLT-4 cells and lowest in peripheral blood lymphocytes. It suggests that pulsed X-rays with various frequencies could be used for target influence on cancer cells being lessdeleterious for normal human cells. |
| first_indexed | 2024-04-10T01:54:44Z |
| format | Article |
| id | doaj.art-cb620b8f98c749d4a1c04353288f945e |
| institution | Directory Open Access Journal |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| last_indexed | 2024-04-10T01:54:44Z |
| publishDate | 2016-02-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj.art-cb620b8f98c749d4a1c04353288f945e2023-03-13T09:05:47ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682016-02-0102454994Diferentefects of pulsed X-rays in MOLT-4 cel line and human peripheral blod lymphocytesS. A. Vasilyev0A. A. Belenko1O. P. Kutenkov2M. A. Bolshakov3I. N. Lebedev4V. V. Rostov5ФГБУ «НИИ медицинской генетики» СО РАМН, г. ТомскТомский государственный университетИнститут сильноточной электроники СО РАН, г. ТомскТомский государственный университет, Институт сильноточной электроники СО РАН, г. ТомскФГБУ «НИИ медицинской генетики» СО РАМН, г. ТомскИнститут сильноточной электроники СО РАН, г. ТомскEffects of ionizing radiation registered in cells after low dose irradiation are still poorly understood. Thus, the aim of this study was to analyze effects of pulsed X-rays on level of radiation-induced DNA double-strand breaks and their repair kinetics in cancer and normalhuman cells in vitro. Analysis of radiation-induced γH2AX and 53BP1 repair foci in MOLT-4 cells with lymphoblastic origin was used for assessment of DNA double-strand breaks (DSB) in these cells. Number of residual radiation-induced γH2AX and 53BP1 foci at 18 hafter irradiation depended on frequency of X-ray pulses: at 8 pulses per second effect was highest in MOLT-4 cells and lowest in peripheral blood lymphocytes. It suggests that pulsed X-rays with various frequencies could be used for target influence on cancer cells being lessdeleterious for normal human cells.https://www.siboncoj.ru/jour/article/view/94импульсно-периодическое рентгеновское излучениемалые дозыгиперрадиочувствительностьфокусы γh2ax и 53bp1двунитевые разрывы днк |
| spellingShingle | S. A. Vasilyev A. A. Belenko O. P. Kutenkov M. A. Bolshakov I. N. Lebedev V. V. Rostov Diferentefects of pulsed X-rays in MOLT-4 cel line and human peripheral blod lymphocytes Сибирский онкологический журнал импульсно-периодическое рентгеновское излучение малые дозы гиперрадиочувствительность фокусы γh2ax и 53bp1 двунитевые разрывы днк |
| title | Diferentefects of pulsed X-rays in MOLT-4 cel line and human peripheral blod lymphocytes |
| title_full | Diferentefects of pulsed X-rays in MOLT-4 cel line and human peripheral blod lymphocytes |
| title_fullStr | Diferentefects of pulsed X-rays in MOLT-4 cel line and human peripheral blod lymphocytes |
| title_full_unstemmed | Diferentefects of pulsed X-rays in MOLT-4 cel line and human peripheral blod lymphocytes |
| title_short | Diferentefects of pulsed X-rays in MOLT-4 cel line and human peripheral blod lymphocytes |
| title_sort | diferentefects of pulsed x rays in molt 4 cel line and human peripheral blod lymphocytes |
| topic | импульсно-периодическое рентгеновское излучение малые дозы гиперрадиочувствительность фокусы γh2ax и 53bp1 двунитевые разрывы днк |
| url | https://www.siboncoj.ru/jour/article/view/94 |
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