Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm
Optical microscopy has vastly expanded the frontiers of structural and functional biology, due to the non-invasive probing of dynamic volumes in vivo. However, traditional widefield microscopy illuminating the entire field of view (FOV) is adversely affected by out-of-focus light scatter. Consequent...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/3/1643 |
| _version_ | 1797487264314425344 |
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| author | Tanveer Teranikar Jessica Lim Toluwani Ijaseun Juhyun Lee |
| author_facet | Tanveer Teranikar Jessica Lim Toluwani Ijaseun Juhyun Lee |
| author_sort | Tanveer Teranikar |
| collection | DOAJ |
| description | Optical microscopy has vastly expanded the frontiers of structural and functional biology, due to the non-invasive probing of dynamic volumes in vivo. However, traditional widefield microscopy illuminating the entire field of view (FOV) is adversely affected by out-of-focus light scatter. Consequently, standard upright or inverted microscopes are inept in sampling diffraction-limited volumes smaller than the optical system’s point spread function (PSF). Over the last few decades, several planar and structured (sinusoidal) illumination modalities have offered unprecedented access to sub-cellular organelles and 4D (3D + time) image acquisition. Furthermore, these optical sectioning systems remain unaffected by the size of biological samples, providing high signal-to-noise (SNR) ratios for objective lenses (OLs) with long working distances (WDs). This review aims to guide biologists regarding planar illumination strategies, capable of harnessing sub-micron spatial resolution with a millimeter depth of penetration. |
| first_indexed | 2024-03-09T23:46:08Z |
| format | Article |
| id | doaj.art-cb63104d71504e9a9ed2049dcb8afeea |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:46:08Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-cb63104d71504e9a9ed2049dcb8afeea2023-11-23T16:43:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-01233164310.3390/ijms23031643Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular RealmTanveer Teranikar0Jessica Lim1Toluwani Ijaseun2Juhyun Lee3Department of Bioengineering, University of Texas at Arlington, Arlington, TX 75022, USADepartment of Bioengineering, University of Texas at Arlington, Arlington, TX 75022, USADepartment of Bioengineering, University of Texas at Arlington, Arlington, TX 75022, USADepartment of Bioengineering, University of Texas at Arlington, Arlington, TX 75022, USAOptical microscopy has vastly expanded the frontiers of structural and functional biology, due to the non-invasive probing of dynamic volumes in vivo. However, traditional widefield microscopy illuminating the entire field of view (FOV) is adversely affected by out-of-focus light scatter. Consequently, standard upright or inverted microscopes are inept in sampling diffraction-limited volumes smaller than the optical system’s point spread function (PSF). Over the last few decades, several planar and structured (sinusoidal) illumination modalities have offered unprecedented access to sub-cellular organelles and 4D (3D + time) image acquisition. Furthermore, these optical sectioning systems remain unaffected by the size of biological samples, providing high signal-to-noise (SNR) ratios for objective lenses (OLs) with long working distances (WDs). This review aims to guide biologists regarding planar illumination strategies, capable of harnessing sub-micron spatial resolution with a millimeter depth of penetration.https://www.mdpi.com/1422-0067/23/3/1643light sheet microscopesuper-resolutionaxially swept light sheetoblique plane illuminationlattice light sheetsub-voxel resolving technique |
| spellingShingle | Tanveer Teranikar Jessica Lim Toluwani Ijaseun Juhyun Lee Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm International Journal of Molecular Sciences light sheet microscope super-resolution axially swept light sheet oblique plane illumination lattice light sheet sub-voxel resolving technique |
| title | Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm |
| title_full | Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm |
| title_fullStr | Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm |
| title_full_unstemmed | Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm |
| title_short | Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm |
| title_sort | development of planar illumination strategies for solving mysteries in the sub cellular realm |
| topic | light sheet microscope super-resolution axially swept light sheet oblique plane illumination lattice light sheet sub-voxel resolving technique |
| url | https://www.mdpi.com/1422-0067/23/3/1643 |
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