Benthic archaea as potential sources of tetraether membrane lipids in sediments across an oxygen minimum zone
<p>Benthic archaea comprise a significant part of the total prokaryotic biomass in marine sediments. Recent genomic surveys suggest they are largely involved in anaerobic processing of organic matter, but the distribution and abundance of these archaeal groups are still largely unknown. Arc...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Copernicus Publications
2018-07-01
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| Series: | Biogeosciences |
| Online Access: | https://www.biogeosciences.net/15/4047/2018/bg-15-4047-2018.pdf |
| _version_ | 1818138758307905536 |
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| author | M. A. Besseling E. C. Hopmans R. C. Boschman J. S. Sinninghe Damsté J. S. Sinninghe Damsté L. Villanueva |
| author_facet | M. A. Besseling E. C. Hopmans R. C. Boschman J. S. Sinninghe Damsté J. S. Sinninghe Damsté L. Villanueva |
| author_sort | M. A. Besseling |
| collection | DOAJ |
| description | <p>Benthic archaea comprise a significant part of the total prokaryotic biomass
in marine sediments. Recent genomic surveys suggest they are largely involved
in anaerobic processing of organic matter, but the distribution and abundance
of these archaeal groups are still largely unknown. Archaeal membrane lipids
composed of isoprenoid diethers or tetraethers (glycerol dibiphytanyl
glycerol tetraether, GDGT) are often used as archaeal biomarkers. Here, we
compare the archaeal diversity and intact polar lipid (IPL) composition in
both surface (0–0.5 cm) and subsurface (10–12 cm) sediments recovered
within, just below, and well below the oxygen minimum zone (OMZ) of the
Arabian Sea. Archaeal 16S rRNA gene amplicon sequencing revealed a
predominance of Thaumarchaeota (Marine Group I, MG-I) in oxygenated
sediments. Quantification of archaeal 16S rRNA and ammonia monoxygenase
(<i>amo</i>A) of Thaumarchaeota genes and their transcripts indicated the
presence of an active in situ benthic population, which coincided with a
high relative abundance of hexose phosphohexose crenarchaeol, a specific
biomarker for living Thaumarchaeota. On the other hand, anoxic surface
sediments within the OMZ and all subsurface sediments were dominated by
archaea belonging to the Miscellaneous Crenarchaeota Group (MCG), the
Thermoplasmatales and archaea of the DPANN (superphylum grouping Micrarchaeota, Diapherotrites, Aenigmarchaeota, Nanohaloarchaeota, Parvarchaeota, Nanoarchaeota, Pacearchaeota and Woesearchaeota). Members of the MCG were diverse, with a dominance of subgroup
MCG-12 in anoxic surface sediments. This coincided with a high relative
abundance of IPL GDGT-0 with an unknown polar head group. Subsurface anoxic
sediments were characterized by higher relative abundance of GDGT-0, -2 and
-3 with dihexose IPL types, GDGT-0 with a cyclopentanetetraol molecule and
hexose, as well as the presence of specific MCG subgroups, suggesting that
these groups could be the biological sources of these archaeal lipids.</p> |
| first_indexed | 2024-12-11T10:17:17Z |
| format | Article |
| id | doaj.art-cb6594f04a7444dbb395080cc854bfe2 |
| institution | Directory Open Access Journal |
| issn | 1726-4170 1726-4189 |
| language | English |
| last_indexed | 2024-12-11T10:17:17Z |
| publishDate | 2018-07-01 |
| publisher | Copernicus Publications |
| record_format | Article |
| series | Biogeosciences |
| spelling | doaj.art-cb6594f04a7444dbb395080cc854bfe22022-12-22T01:11:35ZengCopernicus PublicationsBiogeosciences1726-41701726-41892018-07-01154047406410.5194/bg-15-4047-2018Benthic archaea as potential sources of tetraether membrane lipids in sediments across an oxygen minimum zoneM. A. Besseling0E. C. Hopmans1R. C. Boschman2J. S. Sinninghe Damsté3J. S. Sinninghe Damsté4L. Villanueva5NIOZ Royal Netherlands Institute for Sea Research, Department of Marine Microbiology and Biogeochemistry, and Utrecht University. P.O. Box 59, 1790 AB Den Burg, the NetherlandsNIOZ Royal Netherlands Institute for Sea Research, Department of Marine Microbiology and Biogeochemistry, and Utrecht University. P.O. Box 59, 1790 AB Den Burg, the NetherlandsNIOZ Royal Netherlands Institute for Sea Research, Department of Marine Microbiology and Biogeochemistry, and Utrecht University. P.O. Box 59, 1790 AB Den Burg, the NetherlandsNIOZ Royal Netherlands Institute for Sea Research, Department of Marine Microbiology and Biogeochemistry, and Utrecht University. P.O. Box 59, 1790 AB Den Burg, the NetherlandsUtrecht University, Faculty of Geosciences, Department of Earth sciences, P.O. Box 80.021, 3508 TA Utrecht, the NetherlandsNIOZ Royal Netherlands Institute for Sea Research, Department of Marine Microbiology and Biogeochemistry, and Utrecht University. P.O. Box 59, 1790 AB Den Burg, the Netherlands<p>Benthic archaea comprise a significant part of the total prokaryotic biomass in marine sediments. Recent genomic surveys suggest they are largely involved in anaerobic processing of organic matter, but the distribution and abundance of these archaeal groups are still largely unknown. Archaeal membrane lipids composed of isoprenoid diethers or tetraethers (glycerol dibiphytanyl glycerol tetraether, GDGT) are often used as archaeal biomarkers. Here, we compare the archaeal diversity and intact polar lipid (IPL) composition in both surface (0–0.5 cm) and subsurface (10–12 cm) sediments recovered within, just below, and well below the oxygen minimum zone (OMZ) of the Arabian Sea. Archaeal 16S rRNA gene amplicon sequencing revealed a predominance of Thaumarchaeota (Marine Group I, MG-I) in oxygenated sediments. Quantification of archaeal 16S rRNA and ammonia monoxygenase (<i>amo</i>A) of Thaumarchaeota genes and their transcripts indicated the presence of an active in situ benthic population, which coincided with a high relative abundance of hexose phosphohexose crenarchaeol, a specific biomarker for living Thaumarchaeota. On the other hand, anoxic surface sediments within the OMZ and all subsurface sediments were dominated by archaea belonging to the Miscellaneous Crenarchaeota Group (MCG), the Thermoplasmatales and archaea of the DPANN (superphylum grouping Micrarchaeota, Diapherotrites, Aenigmarchaeota, Nanohaloarchaeota, Parvarchaeota, Nanoarchaeota, Pacearchaeota and Woesearchaeota). Members of the MCG were diverse, with a dominance of subgroup MCG-12 in anoxic surface sediments. This coincided with a high relative abundance of IPL GDGT-0 with an unknown polar head group. Subsurface anoxic sediments were characterized by higher relative abundance of GDGT-0, -2 and -3 with dihexose IPL types, GDGT-0 with a cyclopentanetetraol molecule and hexose, as well as the presence of specific MCG subgroups, suggesting that these groups could be the biological sources of these archaeal lipids.</p>https://www.biogeosciences.net/15/4047/2018/bg-15-4047-2018.pdf |
| spellingShingle | M. A. Besseling E. C. Hopmans R. C. Boschman J. S. Sinninghe Damsté J. S. Sinninghe Damsté L. Villanueva Benthic archaea as potential sources of tetraether membrane lipids in sediments across an oxygen minimum zone Biogeosciences |
| title | Benthic archaea as potential sources of tetraether membrane lipids in sediments across an oxygen minimum zone |
| title_full | Benthic archaea as potential sources of tetraether membrane lipids in sediments across an oxygen minimum zone |
| title_fullStr | Benthic archaea as potential sources of tetraether membrane lipids in sediments across an oxygen minimum zone |
| title_full_unstemmed | Benthic archaea as potential sources of tetraether membrane lipids in sediments across an oxygen minimum zone |
| title_short | Benthic archaea as potential sources of tetraether membrane lipids in sediments across an oxygen minimum zone |
| title_sort | benthic archaea as potential sources of tetraether membrane lipids in sediments across an oxygen minimum zone |
| url | https://www.biogeosciences.net/15/4047/2018/bg-15-4047-2018.pdf |
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