Helicobacter pylori bab characterization in clinical isolates from Bhutan, Myanmar, Nepal and Bangladesh.
Helicobacter pylori BabA is an important outer membrane protein that involves in the attachment to the gastric mucosa and enhances the virulence property of the bacterium. This study was aimed to characterize the bab genotypes, to evaluate its association with cagA, vacA and clinical diseases as wel...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5673166?pdf=render |
| _version_ | 1828526848682754048 |
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| author | Shamshul Ansari Evariste Tshibangu Kabamba Pradeep Krishna Shrestha Hafeza Aftab Thein Myint Lotay Tshering Rabi Prakash Sharma Nwe Ni Than Than Aye Phawinee Subsomwong Tomohisa Uchida Thawee Ratanachu-Ek Ratha-Korn Vilaichone Varocha Mahachai Takashi Matsumoto Junko Akada Yoshio Yamaoka |
| author_facet | Shamshul Ansari Evariste Tshibangu Kabamba Pradeep Krishna Shrestha Hafeza Aftab Thein Myint Lotay Tshering Rabi Prakash Sharma Nwe Ni Than Than Aye Phawinee Subsomwong Tomohisa Uchida Thawee Ratanachu-Ek Ratha-Korn Vilaichone Varocha Mahachai Takashi Matsumoto Junko Akada Yoshio Yamaoka |
| author_sort | Shamshul Ansari |
| collection | DOAJ |
| description | Helicobacter pylori BabA is an important outer membrane protein that involves in the attachment to the gastric mucosa and enhances the virulence property of the bacterium. This study was aimed to characterize the bab genotypes, to evaluate its association with cagA, vacA and clinical diseases as well as degree of gastric inflammation.H. pylori isolates from four countries were subjected for the characterization of bab. The locus specific forward and bab specific reverse primers were used to get the specific products by PCR, which could distinguish the three locus (A, B and C). The histological activities were evaluated according to the Updated Sydney system.In patients from high risk countries (Bhutan and Myanmar) relatively higher frequencies of strains with babA-positivity (91.8% and 90.7%, respectively), babA at locus A (98% and 91.2%, respectively) and with single babA (96.8% and 91.2%, respectively) were found. Strains with two loci occupied were the most prevalent in Bhutan (84.6%), Myanmar (74.7%), Nepal (58.3%) and Bangladesh (56.9%). The genotype babA at locus A/babB at locus B/bab-negative at locus C (babA/babB/-) was the most common genotype isolated from Bhutan (82.7%), Myanmar (58.7%), Nepal (32%) and Bangladesh (31.4%) among all genotypes assessed. This genotype was also associated with the peptic ulcer disease (P = 0.013) when compared to gastritis. babA-positive characteristics and the genotype babA/babB/- exhibited the enhanced histological activities.The higher prevalence of virulence associated babA-positive characteristics and enhanced histological activities in Bhutan than in Myanmar, Nepal and Bangladesh might partly explain why the peoples in Bhutan are at higher risk for developing severe gastric complications. |
| first_indexed | 2024-12-11T21:30:47Z |
| format | Article |
| id | doaj.art-cb6a720843db4eebad774163e538cfbe |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T21:30:47Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cb6a720843db4eebad774163e538cfbe2022-12-22T00:50:11ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011211e018722510.1371/journal.pone.0187225Helicobacter pylori bab characterization in clinical isolates from Bhutan, Myanmar, Nepal and Bangladesh.Shamshul AnsariEvariste Tshibangu KabambaPradeep Krishna ShresthaHafeza AftabThein MyintLotay TsheringRabi Prakash SharmaNwe NiThan Than AyePhawinee SubsomwongTomohisa UchidaThawee Ratanachu-EkRatha-Korn VilaichoneVarocha MahachaiTakashi MatsumotoJunko AkadaYoshio YamaokaHelicobacter pylori BabA is an important outer membrane protein that involves in the attachment to the gastric mucosa and enhances the virulence property of the bacterium. This study was aimed to characterize the bab genotypes, to evaluate its association with cagA, vacA and clinical diseases as well as degree of gastric inflammation.H. pylori isolates from four countries were subjected for the characterization of bab. The locus specific forward and bab specific reverse primers were used to get the specific products by PCR, which could distinguish the three locus (A, B and C). The histological activities were evaluated according to the Updated Sydney system.In patients from high risk countries (Bhutan and Myanmar) relatively higher frequencies of strains with babA-positivity (91.8% and 90.7%, respectively), babA at locus A (98% and 91.2%, respectively) and with single babA (96.8% and 91.2%, respectively) were found. Strains with two loci occupied were the most prevalent in Bhutan (84.6%), Myanmar (74.7%), Nepal (58.3%) and Bangladesh (56.9%). The genotype babA at locus A/babB at locus B/bab-negative at locus C (babA/babB/-) was the most common genotype isolated from Bhutan (82.7%), Myanmar (58.7%), Nepal (32%) and Bangladesh (31.4%) among all genotypes assessed. This genotype was also associated with the peptic ulcer disease (P = 0.013) when compared to gastritis. babA-positive characteristics and the genotype babA/babB/- exhibited the enhanced histological activities.The higher prevalence of virulence associated babA-positive characteristics and enhanced histological activities in Bhutan than in Myanmar, Nepal and Bangladesh might partly explain why the peoples in Bhutan are at higher risk for developing severe gastric complications.http://europepmc.org/articles/PMC5673166?pdf=render |
| spellingShingle | Shamshul Ansari Evariste Tshibangu Kabamba Pradeep Krishna Shrestha Hafeza Aftab Thein Myint Lotay Tshering Rabi Prakash Sharma Nwe Ni Than Than Aye Phawinee Subsomwong Tomohisa Uchida Thawee Ratanachu-Ek Ratha-Korn Vilaichone Varocha Mahachai Takashi Matsumoto Junko Akada Yoshio Yamaoka Helicobacter pylori bab characterization in clinical isolates from Bhutan, Myanmar, Nepal and Bangladesh. PLoS ONE |
| title | Helicobacter pylori bab characterization in clinical isolates from Bhutan, Myanmar, Nepal and Bangladesh. |
| title_full | Helicobacter pylori bab characterization in clinical isolates from Bhutan, Myanmar, Nepal and Bangladesh. |
| title_fullStr | Helicobacter pylori bab characterization in clinical isolates from Bhutan, Myanmar, Nepal and Bangladesh. |
| title_full_unstemmed | Helicobacter pylori bab characterization in clinical isolates from Bhutan, Myanmar, Nepal and Bangladesh. |
| title_short | Helicobacter pylori bab characterization in clinical isolates from Bhutan, Myanmar, Nepal and Bangladesh. |
| title_sort | helicobacter pylori bab characterization in clinical isolates from bhutan myanmar nepal and bangladesh |
| url | http://europepmc.org/articles/PMC5673166?pdf=render |
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