Identification of human cytomegalovirus phosphoprotein 65 in C57BL/6 and BXSB mice as a potential trigger of systemic lupus erythematosus related serum markers
Objective:: To investigate the potential role of human cytomegalovirus lower matrix phosphoprotein 65 (HCMV-pp65) in murine systemic lupus erythematosus (SLE). Methods:: The prokaryotic plasmid pET-28b-pp65 was constructed to express the HCMV-pp65 protein. BXSB mice and C57BL/6 mice were inoculated...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-02-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169115301581 |
| _version_ | 1818190583478353920 |
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| author | Yuan Zhang Ting-Ting Jia Yang Pan Wen-Li Li Yu Sun Jin-Ming Li Lu-Nan Wang |
| author_facet | Yuan Zhang Ting-Ting Jia Yang Pan Wen-Li Li Yu Sun Jin-Ming Li Lu-Nan Wang |
| author_sort | Yuan Zhang |
| collection | DOAJ |
| description | Objective:: To investigate the potential role of human cytomegalovirus lower matrix phosphoprotein 65 (HCMV-pp65) in murine systemic lupus erythematosus (SLE).
Methods:: The prokaryotic plasmid pET-28b-pp65 was constructed to express the HCMV-pp65 protein. BXSB mice and C57BL/6 mice were inoculated with pp65 eukaryotic plasmid pcDNA3.0-pp65 intramuscularly 5 times at 2-week intervals, and then the blood of the mice was subsequently collected via the retro-orbital vein. Indirect ELISAs were used to evaluate the concentration of anti-pp65 immunoglobulin G, anti-double-stranded DNA and antinuclear antibodies. Interleukin-1β and tumor necrosis factor-α were also determined by competitive ELISA. At the same time, 3 major SLE-related circulating microRNAs were examined by quantitative RT-PCR.
Results:: The early production of autoantibodies was observed in pp65-immunized male BXSB as well as C57BL/6 mice. Overexpression of interleukin-1β and tumor necrosis factor-α were detected in pp65-immunized male BXSB mice. Quantitative RT-PCR analyses showed that three SLE related microRNAs (microRNA-126, microRNA-125a, and microRNA-146a) were down-regulated in peripheral blood mononuclear cells of pp65-immunized mice.
Conclusions:: Our findings indicate that HCMV-pp65 immunization strongly triggers the development and progression of SLE-like disease in both BXSB and C57BL/6 mice, which indicates that the immune responses induced by HCMV-pp65 may be involved in the development of SLE. |
| first_indexed | 2024-12-12T00:01:01Z |
| format | Article |
| id | doaj.art-cb6b180bd92f4d81bb391db3492375a2 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-12T00:01:01Z |
| publishDate | 2015-02-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-cb6b180bd92f4d81bb391db3492375a22022-12-22T00:45:14ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912015-02-015213814510.1016/S2221-1691(15)30158-1Identification of human cytomegalovirus phosphoprotein 65 in C57BL/6 and BXSB mice as a potential trigger of systemic lupus erythematosus related serum markersYuan Zhang0Ting-Ting Jia1Yang Pan2Wen-Li Li3Yu Sun4Jin-Ming Li5Lu-Nan Wang6Laboratory Medicine Department, Peking University Third Hospital, Beijing, People's Republic of ChinaNational Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of ChinaInstitute for Infectious Disease and Endemic Disease Control, Beijing Centers for Disease Control and Prevention, Beijing, People's Republic of ChinaNational Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of ChinaNational Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of ChinaNational Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of ChinaNational Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of ChinaObjective:: To investigate the potential role of human cytomegalovirus lower matrix phosphoprotein 65 (HCMV-pp65) in murine systemic lupus erythematosus (SLE). Methods:: The prokaryotic plasmid pET-28b-pp65 was constructed to express the HCMV-pp65 protein. BXSB mice and C57BL/6 mice were inoculated with pp65 eukaryotic plasmid pcDNA3.0-pp65 intramuscularly 5 times at 2-week intervals, and then the blood of the mice was subsequently collected via the retro-orbital vein. Indirect ELISAs were used to evaluate the concentration of anti-pp65 immunoglobulin G, anti-double-stranded DNA and antinuclear antibodies. Interleukin-1β and tumor necrosis factor-α were also determined by competitive ELISA. At the same time, 3 major SLE-related circulating microRNAs were examined by quantitative RT-PCR. Results:: The early production of autoantibodies was observed in pp65-immunized male BXSB as well as C57BL/6 mice. Overexpression of interleukin-1β and tumor necrosis factor-α were detected in pp65-immunized male BXSB mice. Quantitative RT-PCR analyses showed that three SLE related microRNAs (microRNA-126, microRNA-125a, and microRNA-146a) were down-regulated in peripheral blood mononuclear cells of pp65-immunized mice. Conclusions:: Our findings indicate that HCMV-pp65 immunization strongly triggers the development and progression of SLE-like disease in both BXSB and C57BL/6 mice, which indicates that the immune responses induced by HCMV-pp65 may be involved in the development of SLE.http://www.sciencedirect.com/science/article/pii/S2221169115301581Systemic lupus erythematosusAutoantibodyHuman cytomegalovirusLower matrix phosphoprotein 65CytokineMicroRNA |
| spellingShingle | Yuan Zhang Ting-Ting Jia Yang Pan Wen-Li Li Yu Sun Jin-Ming Li Lu-Nan Wang Identification of human cytomegalovirus phosphoprotein 65 in C57BL/6 and BXSB mice as a potential trigger of systemic lupus erythematosus related serum markers Asian Pacific Journal of Tropical Biomedicine Systemic lupus erythematosus Autoantibody Human cytomegalovirus Lower matrix phosphoprotein 65 Cytokine MicroRNA |
| title | Identification of human cytomegalovirus phosphoprotein 65 in C57BL/6 and BXSB mice as a potential trigger of systemic lupus erythematosus related serum markers |
| title_full | Identification of human cytomegalovirus phosphoprotein 65 in C57BL/6 and BXSB mice as a potential trigger of systemic lupus erythematosus related serum markers |
| title_fullStr | Identification of human cytomegalovirus phosphoprotein 65 in C57BL/6 and BXSB mice as a potential trigger of systemic lupus erythematosus related serum markers |
| title_full_unstemmed | Identification of human cytomegalovirus phosphoprotein 65 in C57BL/6 and BXSB mice as a potential trigger of systemic lupus erythematosus related serum markers |
| title_short | Identification of human cytomegalovirus phosphoprotein 65 in C57BL/6 and BXSB mice as a potential trigger of systemic lupus erythematosus related serum markers |
| title_sort | identification of human cytomegalovirus phosphoprotein 65 in c57bl 6 and bxsb mice as a potential trigger of systemic lupus erythematosus related serum markers |
| topic | Systemic lupus erythematosus Autoantibody Human cytomegalovirus Lower matrix phosphoprotein 65 Cytokine MicroRNA |
| url | http://www.sciencedirect.com/science/article/pii/S2221169115301581 |
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