In vivo dynamics of hard tissue-forming cell origins: Insights from Cre/loxP-based cell lineage tracing studies
Bone tissue provides structural support for our bodies, with the inner bone marrow (BM) acting as a hematopoietic organ. Within the BM tissue, two types of stem cells play crucial roles: mesenchymal stem cells (MSCs) (or skeletal stem cells) and hematopoietic stem cells (HSCs). These stem cells are...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Japanese Dental Science Review |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1882761624000024 |
| _version_ | 1797301617466277888 |
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| author | Toshihide Mizoguchi |
| author_facet | Toshihide Mizoguchi |
| author_sort | Toshihide Mizoguchi |
| collection | DOAJ |
| description | Bone tissue provides structural support for our bodies, with the inner bone marrow (BM) acting as a hematopoietic organ. Within the BM tissue, two types of stem cells play crucial roles: mesenchymal stem cells (MSCs) (or skeletal stem cells) and hematopoietic stem cells (HSCs). These stem cells are intricately connected, where BM-MSCs give rise to bone-forming osteoblasts and serve as essential components in the BM microenvironment for sustaining HSCs. Despite the mid-20th century proposal of BM-MSCs, their in vivo identification remained elusive owing to a lack of tools for analyzing stemness, specifically self-renewal and multipotency. To address this challenge, Cre/loxP-based cell lineage tracing analyses are being employed. This technology facilitated the in vivo labeling of specific cells, enabling the tracking of their lineage, determining their stemness, and providing a deeper understanding of the in vivo dynamics governing stem cell populations responsible for maintaining hard tissues. This review delves into cell lineage tracing studies conducted using commonly employed genetically modified mice expressing Cre under the influence of LepR, Gli1, and Axin2 genes. These studies focus on research fields spanning long bones and oral/maxillofacial hard tissues, offering insights into the in vivo dynamics of stem cell populations crucial for hard tissue homeostasis. |
| first_indexed | 2024-03-07T23:25:09Z |
| format | Article |
| id | doaj.art-cb6b5dfa805441a6ba35a2adcc717353 |
| institution | Directory Open Access Journal |
| issn | 1882-7616 |
| language | English |
| last_indexed | 2024-03-07T23:25:09Z |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Japanese Dental Science Review |
| spelling | doaj.art-cb6b5dfa805441a6ba35a2adcc7173532024-02-21T05:24:51ZengElsevierJapanese Dental Science Review1882-76162024-12-0160109119In vivo dynamics of hard tissue-forming cell origins: Insights from Cre/loxP-based cell lineage tracing studiesToshihide Mizoguchi0Oral Health Science Center, Tokyo Dental College, 2–9-18 Kanda-Misakicyo, Chiyoda-ku, Tokyo 101-0061, JapanBone tissue provides structural support for our bodies, with the inner bone marrow (BM) acting as a hematopoietic organ. Within the BM tissue, two types of stem cells play crucial roles: mesenchymal stem cells (MSCs) (or skeletal stem cells) and hematopoietic stem cells (HSCs). These stem cells are intricately connected, where BM-MSCs give rise to bone-forming osteoblasts and serve as essential components in the BM microenvironment for sustaining HSCs. Despite the mid-20th century proposal of BM-MSCs, their in vivo identification remained elusive owing to a lack of tools for analyzing stemness, specifically self-renewal and multipotency. To address this challenge, Cre/loxP-based cell lineage tracing analyses are being employed. This technology facilitated the in vivo labeling of specific cells, enabling the tracking of their lineage, determining their stemness, and providing a deeper understanding of the in vivo dynamics governing stem cell populations responsible for maintaining hard tissues. This review delves into cell lineage tracing studies conducted using commonly employed genetically modified mice expressing Cre under the influence of LepR, Gli1, and Axin2 genes. These studies focus on research fields spanning long bones and oral/maxillofacial hard tissues, offering insights into the in vivo dynamics of stem cell populations crucial for hard tissue homeostasis.http://www.sciencedirect.com/science/article/pii/S1882761624000024Cell lineage tracing analysisCre/loxPSkeletal stem cellsLepRGli1Axin2 |
| spellingShingle | Toshihide Mizoguchi In vivo dynamics of hard tissue-forming cell origins: Insights from Cre/loxP-based cell lineage tracing studies Japanese Dental Science Review Cell lineage tracing analysis Cre/loxP Skeletal stem cells LepR Gli1 Axin2 |
| title | In vivo dynamics of hard tissue-forming cell origins: Insights from Cre/loxP-based cell lineage tracing studies |
| title_full | In vivo dynamics of hard tissue-forming cell origins: Insights from Cre/loxP-based cell lineage tracing studies |
| title_fullStr | In vivo dynamics of hard tissue-forming cell origins: Insights from Cre/loxP-based cell lineage tracing studies |
| title_full_unstemmed | In vivo dynamics of hard tissue-forming cell origins: Insights from Cre/loxP-based cell lineage tracing studies |
| title_short | In vivo dynamics of hard tissue-forming cell origins: Insights from Cre/loxP-based cell lineage tracing studies |
| title_sort | in vivo dynamics of hard tissue forming cell origins insights from cre loxp based cell lineage tracing studies |
| topic | Cell lineage tracing analysis Cre/loxP Skeletal stem cells LepR Gli1 Axin2 |
| url | http://www.sciencedirect.com/science/article/pii/S1882761624000024 |
| work_keys_str_mv | AT toshihidemizoguchi invivodynamicsofhardtissueformingcelloriginsinsightsfromcreloxpbasedcelllineagetracingstudies |