Novel Myco-Coagulant Produced by <i>Lentinus squarrosulus</i> for Removal of Water Turbidity: Fungal Identification and Flocculant Characterization
Several river water fungal strains (RWF-1 to RWF-6) were isolated to investigate the potential of having coagulant properties from the metabolites produced by the fungus. The myco-coagulant produced from the liquid-state process was characterized and tested for flocculation of kaolin water. Molecula...
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MDPI AG
2022-02-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/2/192 |
| _version_ | 1797478928413097984 |
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| author | Nessa Jebun Md Zahangir Alam Abdullah Al Mamun Raha Ahmad Raus |
| author_facet | Nessa Jebun Md Zahangir Alam Abdullah Al Mamun Raha Ahmad Raus |
| author_sort | Nessa Jebun |
| collection | DOAJ |
| description | Several river water fungal strains (RWF-1 to RWF-6) were isolated to investigate the potential of having coagulant properties from the metabolites produced by the fungus. The myco-coagulant produced from the liquid-state process was characterized and tested for flocculation of kaolin water. Molecular identification of the fungal strain isolated from river water and characterization of the myco-coagulant produced by the strain are presented in this paper. The genomic DNA of the fungal 18S ribosomal ribonucleic-acid (rRNA) and 28S rRNA genes were used and the species was identified as <i>Lentinus squarrosulus</i> strain 7-4-2 RWF-5. The characterization of myco-coagulant by Fourier-transform infrared spectroscopy (FTIR) showed that hydroxyl, carbonyl, amide and amine groups as principal functional groups were present in the new myco-coagulant. The mean zeta potential value of the myco-coagulant was −7.0 mV while the kaolin solution was −25.2 mV. Chemical analyses of the extracellular myco-coagulant revealed that it contained total sugar (5.17 g/L), total carbohydrate (237 mg/L), protein (295.4 mg/L), glucosamine (1.152 mg/L); and exhibited cellulase activity (20 units/L) and laccase activity (6.22 units/L). Elemental analyses of C, H, O, N and S showed that the weight fractions of each element in the myco-coagulant was 40.9, 6.0, 49.8, 1.7 and 1.4%, respectively. The myco-coagulant showed 97% flocculation activity at a dose of 1.8 mg/L, indicating good flocculation performance compared to that of polyaluminum chloride (PAC). The present work revealed that the fungal strain, <i>L. squarrosulus</i> 7-4-2 RWF-5 is able to produce cationic bio-coagulant. The flocculation mechanism of the novel myco-coagulant was a combination of polymer bridging and charge neutralization. |
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| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T21:38:36Z |
| publishDate | 2022-02-01 |
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| spelling | doaj.art-cb6b8fcceae542a78fc474ddd3284c892023-11-23T20:38:15ZengMDPI AGJournal of Fungi2309-608X2022-02-018219210.3390/jof8020192Novel Myco-Coagulant Produced by <i>Lentinus squarrosulus</i> for Removal of Water Turbidity: Fungal Identification and Flocculant CharacterizationNessa Jebun0Md Zahangir Alam1Abdullah Al Mamun2Raha Ahmad Raus3Department of Biology, Presidency International School, Chattogram 4217, BangladeshBioenvironmental Engineering Research Centre (BERC), Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia (IIUM), Gombak, Kuala Lumpur 50728, MalaysiaBioenvironmental Engineering Research Centre (BERC), Department of Civil Engineering, Faculty of Engineering, International Islamic University Malaysia (IIUM), Gombak, Kuala Lumpur 50728, MalaysiaBioprocess and Molecular Engineering Research Unit (BPMERU), Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia (IIUM), Gombak, Kuala Lumpur 50728, MalaysiaSeveral river water fungal strains (RWF-1 to RWF-6) were isolated to investigate the potential of having coagulant properties from the metabolites produced by the fungus. The myco-coagulant produced from the liquid-state process was characterized and tested for flocculation of kaolin water. Molecular identification of the fungal strain isolated from river water and characterization of the myco-coagulant produced by the strain are presented in this paper. The genomic DNA of the fungal 18S ribosomal ribonucleic-acid (rRNA) and 28S rRNA genes were used and the species was identified as <i>Lentinus squarrosulus</i> strain 7-4-2 RWF-5. The characterization of myco-coagulant by Fourier-transform infrared spectroscopy (FTIR) showed that hydroxyl, carbonyl, amide and amine groups as principal functional groups were present in the new myco-coagulant. The mean zeta potential value of the myco-coagulant was −7.0 mV while the kaolin solution was −25.2 mV. Chemical analyses of the extracellular myco-coagulant revealed that it contained total sugar (5.17 g/L), total carbohydrate (237 mg/L), protein (295.4 mg/L), glucosamine (1.152 mg/L); and exhibited cellulase activity (20 units/L) and laccase activity (6.22 units/L). Elemental analyses of C, H, O, N and S showed that the weight fractions of each element in the myco-coagulant was 40.9, 6.0, 49.8, 1.7 and 1.4%, respectively. The myco-coagulant showed 97% flocculation activity at a dose of 1.8 mg/L, indicating good flocculation performance compared to that of polyaluminum chloride (PAC). The present work revealed that the fungal strain, <i>L. squarrosulus</i> 7-4-2 RWF-5 is able to produce cationic bio-coagulant. The flocculation mechanism of the novel myco-coagulant was a combination of polymer bridging and charge neutralization.https://www.mdpi.com/2309-608X/8/2/192<i>Lentinus squarrosulus</i>myco-coagulantflocculation mechanismturbiditywater treatment |
| spellingShingle | Nessa Jebun Md Zahangir Alam Abdullah Al Mamun Raha Ahmad Raus Novel Myco-Coagulant Produced by <i>Lentinus squarrosulus</i> for Removal of Water Turbidity: Fungal Identification and Flocculant Characterization Journal of Fungi <i>Lentinus squarrosulus</i> myco-coagulant flocculation mechanism turbidity water treatment |
| title | Novel Myco-Coagulant Produced by <i>Lentinus squarrosulus</i> for Removal of Water Turbidity: Fungal Identification and Flocculant Characterization |
| title_full | Novel Myco-Coagulant Produced by <i>Lentinus squarrosulus</i> for Removal of Water Turbidity: Fungal Identification and Flocculant Characterization |
| title_fullStr | Novel Myco-Coagulant Produced by <i>Lentinus squarrosulus</i> for Removal of Water Turbidity: Fungal Identification and Flocculant Characterization |
| title_full_unstemmed | Novel Myco-Coagulant Produced by <i>Lentinus squarrosulus</i> for Removal of Water Turbidity: Fungal Identification and Flocculant Characterization |
| title_short | Novel Myco-Coagulant Produced by <i>Lentinus squarrosulus</i> for Removal of Water Turbidity: Fungal Identification and Flocculant Characterization |
| title_sort | novel myco coagulant produced by i lentinus squarrosulus i for removal of water turbidity fungal identification and flocculant characterization |
| topic | <i>Lentinus squarrosulus</i> myco-coagulant flocculation mechanism turbidity water treatment |
| url | https://www.mdpi.com/2309-608X/8/2/192 |
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