Expression of cyclooxygenase-1 and cyclooxygenase-2 in normal and pathological human oral mucosa.
Cyclooxigenase (COX) is the rate-limiting enzyme for the conversion of arachidonic acid (AA) to prostaglandins (PGs). Two isoforms of COX have been identified: COX-1 is constitutively expressed in many cells and is involved in cell homeostasis, angiogenesis and cell-cell signalling; COX-2 is not exp...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Via Medica
2011-04-01
|
| Series: | Folia Histochemica et Cytobiologica |
| Online Access: | http://czasopisma.viamedica.pl/fhc/article/view/4169 |
| _version_ | 1819041618556092416 |
|---|---|
| author | Francesco Burruano Silvia Tortorici Angelo Leone Luana Lipari Annamaria Mauro Salvatore Provenzano Aldo Gerbino Maria Buscemi |
| author_facet | Francesco Burruano Silvia Tortorici Angelo Leone Luana Lipari Annamaria Mauro Salvatore Provenzano Aldo Gerbino Maria Buscemi |
| author_sort | Francesco Burruano |
| collection | DOAJ |
| description | Cyclooxigenase (COX) is the rate-limiting enzyme for the conversion of arachidonic acid (AA) to prostaglandins (PGs). Two isoforms of COX have been identified: COX-1 is constitutively expressed in many cells and is involved in cell homeostasis, angiogenesis and cell-cell signalling; COX-2 is not expressed in normal condition however it is strongly expressed in inflammation. The oral cavity is constantly exposed to physical and chemical trauma that could lead to mucosal reactions such as hyperplasia, dysplasia and cancer. Early diagnosis is the most important issue to address for a positive outcome of oral cancer; therefore it would be useful to identify molecular markers whose expression is associated with the various stages of oral cancer progression. Since COX enzyme has been involved, with different mechanisms, in the development and progression of malignancies we decided to investigate the expression and localization of COX-1 and COX-2 in normal human oral mucosa and three different pathologies (hyperplasia, dysplasia and carcinoma) by immunohistochemistry and RT-PCR. COX-1 mRNA and protein have been detected already in normal oral mucosa and their expression progressively increases from normal samples towards hyperplasia, dysplasia and finally carcinoma. On the contrary, COX-2 is not expressed in the normal tissue, starts to be expressed in hyperplasia, reaches the maximum activation in dysplasia and then starts to be downregulated in carcinoma. |
| first_indexed | 2024-12-21T09:27:52Z |
| format | Article |
| id | doaj.art-cb6c51498c0248a6ba0c0a139135ce79 |
| institution | Directory Open Access Journal |
| issn | 0239-8508 1897-5631 |
| language | English |
| last_indexed | 2024-12-21T09:27:52Z |
| publishDate | 2011-04-01 |
| publisher | Via Medica |
| record_format | Article |
| series | Folia Histochemica et Cytobiologica |
| spelling | doaj.art-cb6c51498c0248a6ba0c0a139135ce792022-12-21T19:08:51ZengVia MedicaFolia Histochemica et Cytobiologica0239-85081897-56312011-04-0148455556310.5603/4169Expression of cyclooxygenase-1 and cyclooxygenase-2 in normal and pathological human oral mucosa.Francesco BurruanoSilvia TortoriciAngelo LeoneLuana LipariAnnamaria MauroSalvatore ProvenzanoAldo GerbinoMaria BuscemiCyclooxigenase (COX) is the rate-limiting enzyme for the conversion of arachidonic acid (AA) to prostaglandins (PGs). Two isoforms of COX have been identified: COX-1 is constitutively expressed in many cells and is involved in cell homeostasis, angiogenesis and cell-cell signalling; COX-2 is not expressed in normal condition however it is strongly expressed in inflammation. The oral cavity is constantly exposed to physical and chemical trauma that could lead to mucosal reactions such as hyperplasia, dysplasia and cancer. Early diagnosis is the most important issue to address for a positive outcome of oral cancer; therefore it would be useful to identify molecular markers whose expression is associated with the various stages of oral cancer progression. Since COX enzyme has been involved, with different mechanisms, in the development and progression of malignancies we decided to investigate the expression and localization of COX-1 and COX-2 in normal human oral mucosa and three different pathologies (hyperplasia, dysplasia and carcinoma) by immunohistochemistry and RT-PCR. COX-1 mRNA and protein have been detected already in normal oral mucosa and their expression progressively increases from normal samples towards hyperplasia, dysplasia and finally carcinoma. On the contrary, COX-2 is not expressed in the normal tissue, starts to be expressed in hyperplasia, reaches the maximum activation in dysplasia and then starts to be downregulated in carcinoma.http://czasopisma.viamedica.pl/fhc/article/view/4169 |
| spellingShingle | Francesco Burruano Silvia Tortorici Angelo Leone Luana Lipari Annamaria Mauro Salvatore Provenzano Aldo Gerbino Maria Buscemi Expression of cyclooxygenase-1 and cyclooxygenase-2 in normal and pathological human oral mucosa. Folia Histochemica et Cytobiologica |
| title | Expression of cyclooxygenase-1 and cyclooxygenase-2 in normal and pathological human oral mucosa. |
| title_full | Expression of cyclooxygenase-1 and cyclooxygenase-2 in normal and pathological human oral mucosa. |
| title_fullStr | Expression of cyclooxygenase-1 and cyclooxygenase-2 in normal and pathological human oral mucosa. |
| title_full_unstemmed | Expression of cyclooxygenase-1 and cyclooxygenase-2 in normal and pathological human oral mucosa. |
| title_short | Expression of cyclooxygenase-1 and cyclooxygenase-2 in normal and pathological human oral mucosa. |
| title_sort | expression of cyclooxygenase 1 and cyclooxygenase 2 in normal and pathological human oral mucosa |
| url | http://czasopisma.viamedica.pl/fhc/article/view/4169 |
| work_keys_str_mv | AT francescoburruano expressionofcyclooxygenase1andcyclooxygenase2innormalandpathologicalhumanoralmucosa AT silviatortorici expressionofcyclooxygenase1andcyclooxygenase2innormalandpathologicalhumanoralmucosa AT angeloleone expressionofcyclooxygenase1andcyclooxygenase2innormalandpathologicalhumanoralmucosa AT luanalipari expressionofcyclooxygenase1andcyclooxygenase2innormalandpathologicalhumanoralmucosa AT annamariamauro expressionofcyclooxygenase1andcyclooxygenase2innormalandpathologicalhumanoralmucosa AT salvatoreprovenzano expressionofcyclooxygenase1andcyclooxygenase2innormalandpathologicalhumanoralmucosa AT aldogerbino expressionofcyclooxygenase1andcyclooxygenase2innormalandpathologicalhumanoralmucosa AT mariabuscemi expressionofcyclooxygenase1andcyclooxygenase2innormalandpathologicalhumanoralmucosa |