Effects of High-Fat Diet During Childhood on Precocious Puberty and Gut Microbiota in Mice
Precocious puberty mostly stems from endocrine disorders. However, more and more studies show that a high-fat diet (HFD) is closely related to precocious puberty, but its mechanism is unknown. Since gut microbiota is associated with hormone secretion and obesity, it inspires us to detect the mechani...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.930747/full |
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| author | Tingbei Bo Tingbei Bo Min Liu Min Liu Liqiu Tang Liqiu Tang Jinzhen Lv Jinzhen Lv Jing Wen Dehua Wang Dehua Wang Dehua Wang |
| author_facet | Tingbei Bo Tingbei Bo Min Liu Min Liu Liqiu Tang Liqiu Tang Jinzhen Lv Jinzhen Lv Jing Wen Dehua Wang Dehua Wang Dehua Wang |
| author_sort | Tingbei Bo |
| collection | DOAJ |
| description | Precocious puberty mostly stems from endocrine disorders. However, more and more studies show that a high-fat diet (HFD) is closely related to precocious puberty, but its mechanism is unknown. Since gut microbiota is associated with hormone secretion and obesity, it inspires us to detect the mechanism of gut microbiota in triggering precocious puberty. The model of precocious puberty was established by feeding female mice with an HFD from 21 days old. After puberty, the serum hormone levels, gut microbiome sequencing, and metabolomics were collected. DNA was extracted from feces, and the V3–V4 region of the bacterial 16S rRNA gene was amplified, followed by microbial composition analysis. Subsequently, associations between precocious puberty and the microbiota were determined. We found that (1) HFD after weaning caused precocious puberty, increased serum estradiol, leptin, deoxycholic acid (DCA), and gonadotropin-releasing hormone (GnRH) in the hypothalamus; (2) Through correlation analysis, we found that GnRH was positively correlated with Desulfovibrio, Lachnoclostridium, GCA-900066575, Streptococcus, Anaerotruncus, and Bifidobacterium, suggesting that these bacteria may have a role in promoting sexual development. (3) “HFD-microbiota” transplantation promoted the precocious puberty of mice. (4) Estrogen changes the composition and proportion of gut microbiota and promotes precocious puberty. Therefore, the effect of HFD on precocious puberty is regulated by the interaction of gut microbiota and hormones. |
| first_indexed | 2024-04-13T04:45:25Z |
| format | Article |
| id | doaj.art-cb6f573455ab4a6b9fc417158e2b7c77 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T04:45:25Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-cb6f573455ab4a6b9fc417158e2b7c772022-12-22T03:01:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-07-011310.3389/fmicb.2022.930747930747Effects of High-Fat Diet During Childhood on Precocious Puberty and Gut Microbiota in MiceTingbei Bo0Tingbei Bo1Min Liu2Min Liu3Liqiu Tang4Liqiu Tang5Jinzhen Lv6Jinzhen Lv7Jing Wen8Dehua Wang9Dehua Wang10Dehua Wang11State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaCAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaCAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaCAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaCAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, ChinaCollege of Life and Environmental Science, Wenzhou University, Wenzhou, ChinaState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaSchool of Life Sciences, Shandong University, Qingdao, ChinaCAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, ChinaPrecocious puberty mostly stems from endocrine disorders. However, more and more studies show that a high-fat diet (HFD) is closely related to precocious puberty, but its mechanism is unknown. Since gut microbiota is associated with hormone secretion and obesity, it inspires us to detect the mechanism of gut microbiota in triggering precocious puberty. The model of precocious puberty was established by feeding female mice with an HFD from 21 days old. After puberty, the serum hormone levels, gut microbiome sequencing, and metabolomics were collected. DNA was extracted from feces, and the V3–V4 region of the bacterial 16S rRNA gene was amplified, followed by microbial composition analysis. Subsequently, associations between precocious puberty and the microbiota were determined. We found that (1) HFD after weaning caused precocious puberty, increased serum estradiol, leptin, deoxycholic acid (DCA), and gonadotropin-releasing hormone (GnRH) in the hypothalamus; (2) Through correlation analysis, we found that GnRH was positively correlated with Desulfovibrio, Lachnoclostridium, GCA-900066575, Streptococcus, Anaerotruncus, and Bifidobacterium, suggesting that these bacteria may have a role in promoting sexual development. (3) “HFD-microbiota” transplantation promoted the precocious puberty of mice. (4) Estrogen changes the composition and proportion of gut microbiota and promotes precocious puberty. Therefore, the effect of HFD on precocious puberty is regulated by the interaction of gut microbiota and hormones.https://www.frontiersin.org/articles/10.3389/fmicb.2022.930747/fullprecocious pubertygut microbiotamicehigh-fat dietmating behavior tendency |
| spellingShingle | Tingbei Bo Tingbei Bo Min Liu Min Liu Liqiu Tang Liqiu Tang Jinzhen Lv Jinzhen Lv Jing Wen Dehua Wang Dehua Wang Dehua Wang Effects of High-Fat Diet During Childhood on Precocious Puberty and Gut Microbiota in Mice Frontiers in Microbiology precocious puberty gut microbiota mice high-fat diet mating behavior tendency |
| title | Effects of High-Fat Diet During Childhood on Precocious Puberty and Gut Microbiota in Mice |
| title_full | Effects of High-Fat Diet During Childhood on Precocious Puberty and Gut Microbiota in Mice |
| title_fullStr | Effects of High-Fat Diet During Childhood on Precocious Puberty and Gut Microbiota in Mice |
| title_full_unstemmed | Effects of High-Fat Diet During Childhood on Precocious Puberty and Gut Microbiota in Mice |
| title_short | Effects of High-Fat Diet During Childhood on Precocious Puberty and Gut Microbiota in Mice |
| title_sort | effects of high fat diet during childhood on precocious puberty and gut microbiota in mice |
| topic | precocious puberty gut microbiota mice high-fat diet mating behavior tendency |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.930747/full |
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