Suppressing postcollection lysophosphatidic acid metabolism improves the precision of plasma LPA quantification
Abstract: Lysophosphatidic acid (LPA) is a potent signaling lipid, and state-dependent alterations in plasma LPA make it a promising diagnostic marker for various diseases. However, plasma LPA concentrations vary widely among reports, even under normal conditions. These variations can be attributed,...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227521000092 |
| _version_ | 1819100191196708864 |
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| author | Kuniyuki Kano Hirotaka Matsumoto Nozomu Kono Makoto Kurano Yutaka Yatomi Junken Aoki |
| author_facet | Kuniyuki Kano Hirotaka Matsumoto Nozomu Kono Makoto Kurano Yutaka Yatomi Junken Aoki |
| author_sort | Kuniyuki Kano |
| collection | DOAJ |
| description | Abstract: Lysophosphatidic acid (LPA) is a potent signaling lipid, and state-dependent alterations in plasma LPA make it a promising diagnostic marker for various diseases. However, plasma LPA concentrations vary widely among reports, even under normal conditions. These variations can be attributed, at least in part, to the artificial metabolism of LPA after blood collection. Here, we aimed to develop an optimized plasma preparation method that reflects the concentration of LPA in the circulating blood. The main features of the devised method were suppression of both LPA production and degradation after blood collection by keeping whole blood samples at low temperature followed by the addition of an autotaxin inhibitor to plasma samples. Using this devised method, the LPA level did not change for 30 min after blood collection. Also, human and mouse LPA levels were found to be much lower than those previously reported, ranging from 40 to 50 nM with minimal variation across the individual. Finally, the increased accuracy made it possible to detect circadian rhythms in the levels of certain LPA species in mouse plasma. These results demonstrate the usefulness of the devised plasma preparation method to determine accurate plasma LPA concentrations. |
| first_indexed | 2024-12-22T00:58:51Z |
| format | Article |
| id | doaj.art-cb71ac2ab8934d2da7c4365a9589d3f0 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-22T00:58:51Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-cb71ac2ab8934d2da7c4365a9589d3f02022-12-21T18:44:15ZengElsevierJournal of Lipid Research0022-22752021-01-0162100029Suppressing postcollection lysophosphatidic acid metabolism improves the precision of plasma LPA quantificationKuniyuki Kano0Hirotaka Matsumoto1Nozomu Kono2Makoto Kurano3Yutaka Yatomi4Junken Aoki5Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan; Laboratory of Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-Ku, Sendai, Japan; AMED-LEAP, Japan Science and Technology Corporation, Kawaguchi, Saitama, JapanLaboratory of Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-Ku, Sendai, JapanDepartment of Health Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo, JapanAMED-LEAP, Japan Science and Technology Corporation, Kawaguchi, Saitama, Japan; Department of Clinical Laboratory, University of Tokyo Hospital, Bunkyo-ku, Tokyo, JapanAMED-LEAP, Japan Science and Technology Corporation, Kawaguchi, Saitama, Japan; Department of Clinical Laboratory, University of Tokyo Hospital, Bunkyo-ku, Tokyo, JapanDepartment of Health Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan; Laboratory of Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-Ku, Sendai, Japan; AMED-LEAP, Japan Science and Technology Corporation, Kawaguchi, Saitama, Japan; For correspondence: Junken Aoki.Abstract: Lysophosphatidic acid (LPA) is a potent signaling lipid, and state-dependent alterations in plasma LPA make it a promising diagnostic marker for various diseases. However, plasma LPA concentrations vary widely among reports, even under normal conditions. These variations can be attributed, at least in part, to the artificial metabolism of LPA after blood collection. Here, we aimed to develop an optimized plasma preparation method that reflects the concentration of LPA in the circulating blood. The main features of the devised method were suppression of both LPA production and degradation after blood collection by keeping whole blood samples at low temperature followed by the addition of an autotaxin inhibitor to plasma samples. Using this devised method, the LPA level did not change for 30 min after blood collection. Also, human and mouse LPA levels were found to be much lower than those previously reported, ranging from 40 to 50 nM with minimal variation across the individual. Finally, the increased accuracy made it possible to detect circadian rhythms in the levels of certain LPA species in mouse plasma. These results demonstrate the usefulness of the devised plasma preparation method to determine accurate plasma LPA concentrations.http://www.sciencedirect.com/science/article/pii/S0022227521000092lysophosphatidic acidplasmaclinical specimenmetabolismautotaxinLC-MS |
| spellingShingle | Kuniyuki Kano Hirotaka Matsumoto Nozomu Kono Makoto Kurano Yutaka Yatomi Junken Aoki Suppressing postcollection lysophosphatidic acid metabolism improves the precision of plasma LPA quantification Journal of Lipid Research lysophosphatidic acid plasma clinical specimen metabolism autotaxin LC-MS |
| title | Suppressing postcollection lysophosphatidic acid metabolism improves the precision of plasma LPA quantification |
| title_full | Suppressing postcollection lysophosphatidic acid metabolism improves the precision of plasma LPA quantification |
| title_fullStr | Suppressing postcollection lysophosphatidic acid metabolism improves the precision of plasma LPA quantification |
| title_full_unstemmed | Suppressing postcollection lysophosphatidic acid metabolism improves the precision of plasma LPA quantification |
| title_short | Suppressing postcollection lysophosphatidic acid metabolism improves the precision of plasma LPA quantification |
| title_sort | suppressing postcollection lysophosphatidic acid metabolism improves the precision of plasma lpa quantification |
| topic | lysophosphatidic acid plasma clinical specimen metabolism autotaxin LC-MS |
| url | http://www.sciencedirect.com/science/article/pii/S0022227521000092 |
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